Characteristic reaction of the mouse adrenal cortical cell to osmium impregnation.

On the Internal Polarity of the Golgi Apparatus with Special Regard to Its Relationship to GERL

“…In all these cells, only the cisternae of 1-3 stacks and vesicles on the cis side of the Golgi apparatus stained heavily; the cisternae of 1-3 stacks of the trans side were almost negative (8,9).…”

“…When tissues were immersed in an 0s04 -water solution (pH 6.0-6.7) for 40-48 hr at 40°C after fixation with 1% Os04 buffered at pH 7.4 for 1-2 hr, the cisternae of the Golgi apparatus stained intensely in the various types of cells examined, including anterior pituitary cells, thyroid follicle cells, exocrine pancreatic cells, hepatocytes, duodenal epithelial cells, adrenal cortical cells, Leydig cells, epithelial cells of vas deferens, and theca cells of mouse ovary (1,8,9). In all these cells, only the cisternae of 1-3 stacks and vesicles on the cis side of the Golgi apparatus stained heavily; the cisternae of 1-3 stacks of the trans side were almost negative (8,9).…”