On the Internal Polarity of the Golgi Apparatus with Special Regard to Its Relationship to GERL

Fujita, Hisao; Sawano, Fumio

doi:10.1177/31.1a_suppl.6131092

Cited by 11 publications

(3 citation statements)

References 11 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acid phosphatase activity is also found in Golgi cisternae by using P-glycerophosphate as a substrate. Acid phosphatase activity in Golgi cisternae has been demonstrated in many other cell types (Goldfisher, 1982;Fujita and Swano, 1983). It is clear from these studies and previous observations by Enders and Wimsatt (1971) that the contents of most uncoated endocytic vesicles pass through the lysosomal compartment.…”

Section: Histochemistrymentioning

confidence: 62%

Synthesis and transport studies of the intrasyncytial lamina: An unusual placental basement membrane in the little brown bat, Myotis lucifugus

Cukierski

1987

Am. J. Anat.

View full text Add to dashboard Cite

The chorioallantoic placenta of Myotis lucifugus undergoes a transition from endotheliochorial to hemochorial. The original maternal endothelial basement membrane is incorporated into the apical portion of the syncytial trophoblast, where it persists until term. This intrasyncytial lamina is separated from the maternal blood by thin ectoplasmic projections of the syncytial trophoblast that project through the lamina and spread over the surface, completely engulfing it. While there appear to be direct channels, at junctions of the ectoplasmic processes, from the maternal blood to the intrasyncytial lamina, perfusion studies using the electron-dense tracers alcian blue, ruthenium red, and Thorotrast show that these channels are physiologically closed. In contrast, lanthanum nitrate was able to gain access to the lamina via the extracellular channels. The endocytic uptake of the tracers was similar. These studies suggest several pathways for substances to cross the ectoplasmic zone and the intrasyncytial lamina. Substances may gain direct access to the lamina via extracellular channels, reach the lamina by vesicular transport, or bypass the lamina completely through fenestrations within the lamina. Autoradiographic studies show that the syncytial trophoblast synthesizes portions of the intrasyncytial lamina, demonstrating its partial fetal origin. How long the original maternal components persist and the functional significance of the intrasyncytial lamina are unknown. Possible functions of the lamina include increased surface area of the apical plasmalemma, selective filtration, structural support, and maintenance of cell polarity.

show abstract

Section: Histochemistrymentioning

confidence: 62%

Synthesis and transport studies of the intrasyncytial lamina: An unusual placental basement membrane in the little brown bat, Myotis lucifugus

Cukierski

1987

Am. J. Anat.

View full text Add to dashboard Cite

show abstract

“…Dyctiosomes have shown enzymatic activity, especially in the cisternae of the trans side of Golgi apparatus, such as in other cell types (6).…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural localization of acid phosphatase activity in the kidney sac nephrocytes of helix aspersa.

Sánchez‐Aguayo¹,

Hidalgo²,

Cortés³

et al. 1984

Acta Histochem. Cytochem

View full text Add to dashboard Cite

“…Nonetheless, our results suggest that the apparatus is organized in "cis-trans" terms much like that of other neurons, in which acid phosphatase and TPPase show their strongest cytochemical activities in distinct, although adjacent, sacs near the trans Golgi face (e.g., Novikoff et al, 1971;Novikoff and Novikoff, 1977). However, as in several other cases (cf., Broadwell, 1980;Fujita and Sawano, 1983;Hand and Region near the apex of the cell body in a rod cell from a postembedding immunocytochemical preparation with the anti-p38 antibodies; 5 nm gold particles are clustered over a region of small vesicles and tubules (arrow). Note the absence of labeling over the outer segment (0s) and the mitochondria (M).…”

Section: Immunocytochernist Ymentioning

confidence: 99%

Involvement of the Golgi apparatus in sorting of materials to opposite ends of frog rod retinal photoreceptors

Schmied

Holtzman

1989

J. Neurobiol.

View full text Add to dashboard Cite

We have studied the rod cells of retinas of Rana pipiens by phosphatase cytochemistry and immunocytochemistry. We find that the Golgi apparatus of these cells, although different in its intracellular distribution from that of other neurons, has a cis-trans organization like that of other neurons as regards morphological features and the distribution of phosphatase activities. Antibodies against opsin bind to several sacs of the rod Golgi apparatus, especially those at the trans side of the Golgi stack. This suggests that Golgi involvement in the packaging of opsin for eventual delivery to the photoreceptive outer segments of the cell involves passage through trans Golgi systems. Proteins destined for the opposite end of the cell--the presynaptic terminal--also seem to pass through trans Golgi systems, as is indicated both by immunocytochemical localization of the synaptic vesicle protein p38 (synaptophysin) and by the presence of thiamine pyrophosphatase activity in some of the synaptic vesicles. Our findings suggest that sorting of membrane proteins destined for opposite ends of the photoreceptor takes place in systems at or near the trans Golgi face.

show abstract

On the Internal Polarity of the Golgi Apparatus with Special Regard to Its Relationship to GERL

Abstract: The authors emphasized that from the cytochemical standpoint the Golgi apparatus has an internal polarity and that GERL is a part of this organelle.

Cited by 11 publications

References 11 publications

Synthesis and transport studies of the intrasyncytial lamina: An unusual placental basement membrane in the little brown bat, Myotis lucifugus

Synthesis and transport studies of the intrasyncytial lamina: An unusual placental basement membrane in the little brown bat, Myotis lucifugus

Ultrastructural localization of acid phosphatase activity in the kidney sac nephrocytes of helix aspersa.

Involvement of the Golgi apparatus in sorting of materials to opposite ends of frog rod retinal photoreceptors

Contact Info

Product

Resources

About