Characterisation of volatiles produced from Bacillus megaterium YFM3.25 and their nematicidal activity against Meloidogyne incognita

Huang, Ying; Xu, Changjie; Ma, Lan; Zhang, Ke‐Qin; Duan, Changqun; Mo, Ming-He

doi:10.1007/s10658-009-9550-z

Cited by 145 publications

(80 citation statements)

References 13 publications

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“…Reduction in tomato infectivity (numbers of galls) by M. incognita J2 after exposure to the VOCs of F. oxysporum was demonstrated by Freire et al (2012). Huang et al (2010) also found a reduction in the number of galls and eggs of M. incognita after exposure to volatiles of Bacillus megaterium YFM3.25. Metabolites produced by B. megaterium also caused a significant reduction in the number of root galls and eggs of nematodes of M. exigua in a study by Oliveira et al (2007).…”

Section: Discussionmentioning

confidence: 79%

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Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pimenta

Ferreira

Pedroso

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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Since volatiles do not leave residues in food and cause low toxicity to humans, they can act as a skeleton for new nematicidal molecules, once they have demonstrated activity against phytonematodes. The aim of this study was to evaluate the effect of volatile organic compounds (VOCs) released by wood-associated fungi on controling second-stage juveniles (J2) of Meloidogyne incognita. All 28 wood-associated fungi isolates produced VOCs which caused in excess of 76 % immobility in exposed J2 of M. incognita. The fungus isolate VOCs also caused significant mortality compared to control when J2 were exposed to them. After 6 h of exposure, the fungus species Epicoccum nigrum and Schizophyllum commune produced VOCs that immobilized exposed J2 compounds, in relation to the control. When J2 were inoculated into tomato under greenhouse conditions, the M. incognita infectivity and reproduction were reduced by exposure to the VOCs. Volatiles produced by most of the fungi isolates and analyzed by gas chromatograph coupled to a mass spectrometer (CG-MS), included alcohols, esters, terpenes, and ketones. Certain compounds appeared only in S. commune or in E. nigrum suggesting their involvement in the high level of damage caused to nematode reproduction on tomato. Volatiles emitted by wood fungi demonstrated another mode of action of these microorganisms in nature.

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Section: Discussionmentioning

confidence: 79%

“…The production of volatile emissions by E. nigrum, S. commune, P. chrysosporium, Nisgrospora sp., and Lasiodiplodia sp. toxic to nematodes have not yet been described; however, many other fungi species produce volatiles toxic to plant pathogenic nematode and to other plant pathogens (Campos et al, 2010;Huang et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Pimenta

Ferreira

Pedroso

et al. 2017

Sci. agric. (Piracicaba, Braz.)

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“…Another study by Mekete et al (2009) revealed that B. pumilus reduced root-galling and egg-mass numbers of M. incognita on Ethiopian coffee. Bacillus megaterium reduced egg hatching and the number of second-stage juvenile (J2) of Meloidogyne incognita (Huang et al, 2010), which was likely resulted from nematotoxic compounds or extracellular hydrolytic enzymes of bacteria destroying nematode eggshell and juvenile cuticle.…”

Section: Resultsmentioning

confidence: 99%

Effect of some plant growth-promoting rhizobacteria strains on root-knot nematode, Meloidogyne incognita, on tomatoes

Cetintas

Küsek

Fateh

2018

Egypt J Biol Pest Control

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Root-knot nematode, Meloidogyne incognita, is one of the main problems in vegetable-growing regions, decreasing yield quality and quantity worldwide. Root-knot nematode management tactics mostly involve chemical control which is a threat to the environment, consequences to human health. Biological control of nematodes is considered to be one of the best alternatives to the chemical control. In this study, the effect of some bacterial isolates against M. incognita was determined on tomato in the greenhouse. The trial was designed as a randomized complete block, consisting of 15 plant growth-promoting rhizobacteria (PGPR) strains and 2 control groups (−, +) with 10 replications, total of 170 pots. Two days after transplanting the bacteria-treated tomato (Solanum lycopersicum cv. 56-56 F1) seedlings in the sterile soil in pots, the plants were inoculated with 1000 eggs or J2s of M. incognita/pot. At the end of a 90-day plant-growing period, isolate ZHA90 of Bacillus pumilus decreased plant root galling which, in turn, increased plant height, shoot fresh and dry weight, and root fresh weight. Isolates ZHA296 and ZHA178 of Paenibacillus castaneae reduced the number of egg masses and root galling with no effects on plant growth compared to the control (+). While the isolate ZHA17 of Mycobacterium immunogenum increased plant height and shoot fresh weight, ZHA57 of the same bacterium enhanced significantly only plant height. Results indicated that among 15 bacterial strains studied, ZHA296 and ZHA178 of P. castaneae and ZHA17 and ZHA57 of M. immunogenum were identified as the promising biocontrol agents for the future nematode management tactics.

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“…DMDS was first described as produced by bacteria isolated from decomposing chicken (Freeman et al, 1976). Surprisingly, DMDS, along with other VOCs emitted by rhizobacteria Pseudomonas fluorescens B-4117 and Serratia plymuthica IC1270, was found to exert antimicrobial and nematicidal activity Wang et al, 2009;Huang et al, 2010;Dandurishvili et al, 2011) and to inhibit the cell-cell communication quorumsensing network of various bacteria . Furthermore, DMDS functions as a plant defense compound against nonspecialist herbivores feeding on Allium porrum (Dugravot et al, 2003(Dugravot et al, , 2004 and as both an oviposition repellent and an attractant to different natural enemies of the cabbage root fly Delia radicum (Ferry et al, 2007(Ferry et al, , 2009.…”

Section: Discussionmentioning

confidence: 99%

Dimethyl Disulfide Produced by the Naturally Associated Bacterium Bacillus sp B55 Promotes Nicotiana attenuata Growth by Enhancing Sulfur Nutrition

et al. 2013

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Bacillus sp B55, a bacterium naturally associated with Nicotiana attenuata roots, promotes growth and survival of wild-type and, particularly, ethylene (ET)-insensitive 35S-ethylene response1 (etr1) N. attenuata plants, which heterologously express the mutant Arabidopsis thaliana receptor ETR1-1. We found that the volatile organic compound (VOC) blend emitted by B55 promotes seedling growth, which is dominated by the S-containing compound dimethyl disulfide (DMDS). DMDS was depleted from the headspace during cocultivation with seedlings in bipartite Petri dishes, and 35 S was assimilated from the bacterial VOC bouquet and incorporated into plant proteins. In wild-type and 35S-etr1 seedlings grown under different sulfate (SO 4 22 ) supply conditions, exposure to synthetic DMDS led to genotype-dependent plant growth promotion effects. For the wild type, only S-starved seedlings benefited from DMDS exposure. By contrast, growth of 35S-etr1 seedlings, which we demonstrate to have an unregulated S metabolism, increased at all SO 4 22 supply rates. Exposure to B55 VOCs and DMDS rescued many of the growth phenotypes exhibited by ET-insensitive plants, including the lack of root hairs, poor lateral root growth, and low chlorophyll content. DMDS supplementation significantly reduced the expression of S assimilation genes, as well as Met biosynthesis and recycling. We conclude that DMDS by B55 production is a plant growth promotion mechanism that likely enhances the availability of reduced S, which is particularly beneficial for wild-type plants growing in S-deficient soils and for 35S-etr1 plants due to their impaired S uptake/assimilation/metabolism.

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Characterisation of volatiles produced from Bacillus megaterium YFM3.25 and their nematicidal activity against Meloidogyne incognita

Cited by 145 publications

References 13 publications

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Wood-associated fungi produce volatile organic compounds toxic to root-knot nematode

Effect of some plant growth-promoting rhizobacteria strains on root-knot nematode, Meloidogyne incognita, on tomatoes

Dimethyl Disulfide Produced by the Naturally Associated Bacterium Bacillus sp B55 Promotes Nicotiana attenuata Growth by Enhancing Sulfur Nutrition

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