Characterisation of two Ukrainian populations of Globodera pallida in terms of their virulence and mtDNA, and the biological assessment of a new resistant cultivar Vales Everest

Abstract

Following the discovery of Globodera pallida in the Uzhhorod region (Zakarpats'ka oblast') of Ukraine, two populations from this region were further examined. Firstly, their virulence was assessed in relation to two sources of resistance to G. pallida and to other G. pallida populations from Europe and South America. The results showed that the Ukrainian populations were very similar in their virulence to the other European populations. One of the test host genotypes was a new cultivar derived from Solanum tuberosum spp. andigena CPC2802, which proved to be more highly resistant than previously available partially resistant cultivars. The second experiment was a comparison of the mitochondrial Cytochrome B gene from the Ukrainian populations with other European and South American populations. The data indicated that the Ukrainian populations were similar to other European populations.

Potato cyst nematodes Globodera rostochiensis and G. pallida

“…Europe has subsequently acted as a secondary distribution centre for PCN, most likely due to distribution of contaminated seed potato material (Hockland et al., 2012). Consistent with this, genotyping of populations occurring outside Europe shows “typical” European population types rather than exotic types from the centre of origin (e.g., Pylypenko et al., 2008).…”

An evaluation of the implications of virulence in non-European populations of Globodera pallida and G. rostochiensis for potato cultivation in Europe

“…The accession of several countries into the EU in recent years has not been accompanied by a detailed assessment of the consequences of an extended gene or virulence pool now possible with new trading patterns. However, Pylypenko et al (2008) investigated the virulence of two Ukrainian populations of G. pallida and concluded they were more similar to other European rather than South American populations, but generally such research, with either species of PCN, is lacking.…”

Identification, phylogeny and phylogeography of circumfenestrate cyst nematodes (Nematoda: Heteroderidae) as inferred from analysis of ITS-rDNA

“…Distinct genetic differences among different G. pallida populations spanning Europe and those found in South America were revealed by sequence and phylogenetic analyses of the ITS-rRNA (Blok et al, 1998;Subbotin et al, 2000;Madani et al, 2010), cytb (Picard et al, 2007;Plantard et al, 2008;Pylypenko et al, 2008;Madani et al, 2010), PCR-ITS-RFLP (Ayub & Rumpenhorst, 2000;Grenier et al, 2001), simple sequence repeat primer analysis (Blok & Phillips, 1995), PCR-RAPD (Bendezu et al, 1988;Blok et al, 1997;Grenier et al, 2001;Rumpenhorst & Ayub, 2001), IEF of proteins (Rumpenhorst & Ayub, 2001), 2-DGE of proteins , and satellite DNA analysis Plantard et al, 2008). The results of phylogenetic analysis of the ITS-rRNA gene sequences of Globodera species parasitising solanaceous plants suggested the presence of a separate group of populations closely related to G. pallida, which might represent an undescribed Globodera species (Subbotin et al, 2000).…”