Characterisation of three mosquitocidal Bacillus strains isolated from mangrove forest

Geetha, I.; Prabakaran, G.; Paily, K. P.; Manonmani, A.M.; Balaraman, K

doi:10.1016/j.biocontrol.2007.04.003

Cited by 30 publications

(22 citation statements)

References 22 publications

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“…Gupta and Vyas (1989) reported a strain of B. subtilis capable of causing mortality of larvae of Anopheles culicifacies, the primary vector of malaria in India. Recently, Das and Mukherjee (2006), Geetha et al (2007) and Geetha and Manonmani (2008) have reported mosquito larvicidal and pupicidal activities of cyclic lipopeptides (CLPs) secreted by B. subtilis strains. B. cereus also a gram positive, spore forming rod shaped bacteria used for biological control agent widely available in soil environment.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India

2013

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Samples collected from the mangroves of Vellar estuary yielded a mosquitocidal bacterium, whose secondary metabolites exhibited mosquito larvicidal and pupicidal activity. The bacterium was isolated using standard microbiological methods and identified using classical biochemical tests. The mosquitocidal bacterium was identified as Bacillus subtilis, Bacillus thuringiensis, Bacillus sphaericus and Bacillus cereus. Mosquitocidal metabolite(s) was separated from the culture supernatant of the bacterium and its efficacy was against the larval and pupal stages of two different species of mosquitoes and determined in terms of LC 50 and LC 90 . Mosquito larvicidal activity in terms of LC 50 against Anopheleus stephensi and Aedes aegypti was 4.374 and 7.406 ll/ml and its pupicidal activity was 4.928 and 9.865 ll/ml, respectively. The present study proved that the mosquitocidal properties of the Bacillus species isolated from mangroves of Vellar estuary was evaluated as target species of mosquito vectors. This is an ideal eco-friendly approach for the vector control programs.

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Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India

2013

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“…Pupae/larvae (IV instar) of the adult mosquitoes of Culex quinquefasciatus were obtained from the rearing and colonization facility of VCRC and exposed to lethal concentration 50 (LC 50) of B-426 (511.5 µg/mL) [7] and B-471 (27 µL/mL). [8] This was done by adding the metabolite containing toxic proteins to disposable cups each containing 25 larvae or 25 pupae with 100 mL of chlorine-free tap water. Pupae were treated with the toxin of B-426 and larvae with the proteins of B-471.…”

Section: Treatment Of Pupae and Larvae With Lc 50 Dosementioning

confidence: 99%

“…[3,4] Although these agents have been used extensively in the field, report of development of resistance by mosquito larvae to B. sphaericus [5,6] has led to the search of new microbial agents or their metabolites. At the Vector Control Research Centre (VCRC), Pondicherry search for such new microbial agents or their metabolites for mosquitocidal activity has resulted in the identification of two potential bacterial agents viz., Pseudomonas fluorescens (B-426) [7] and Bacillus subtilis (B-471), [8] whose metabolites could kill the mosquito pupae and larvae.…”

Section: Introductionmentioning

confidence: 99%

Binding Sites of Mosquitocidal Toxins ofPseudomonas FluorescensandBacillus Subtilison Pupae and Larvae ofCulex quinquefasciatus

Mary

Paily

Hoti

et al. 2014

Journal of Immunoassay and Immunochemistry

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Two of the potential bacterial isolates, viz., Pseudomonas fluorescens (VCRC B-426) and Bacillus subtilis (VCRC B-471) whose toxins kill the mosquito pupae/larvae have been identified at our center. As the mode of action of these bacteria are not known, an attempt was made to find out the binding sites of the toxic proteins through immunological methods. Antibodies were raised in BALB/c mice and egg yolk system of chicken layers against the mosquitocidal proteins. The antibodies showed specific binding on to the cephalic and thoracic cuticle of the pupae as well as the paddles of the larvae, indicating the binding of the mosquitocidal proteins.

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“…Soil samples preparation was performed as described by Geetha et al (2007) with minor modifications. In brief, 1 g of soil was weighed, transferred to a vial containing 10 ml of sterile water, and kept on a rotary shaker (Edmund Buhler, Germany) at 150 rpm for 30 min to dislodge bacterial cells from the soil particles.…”

Section: Isolation Of Bacterial Strainsmentioning

confidence: 99%

“…Microbial pesticides are considered as new strategies that present advantages as they are eco-friendly and specific to the target organisms. Among others, Bacillus thuringiensis and Bacillus sphaericus toxins are being used widely as larvicidal bacteria for mosquito control (Balaraman 1995;Lee and Zairi 2005;Medeiros et al 2005;Armengol et al 2006;Geetha et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Biocontrol potential of a Bacillus subtilis strain against Bactrocera oleae

Mostakim¹,

Abed

Iraqui³

et al. 2011

Ann Microbiol

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Within the Mediterranean basin, pest infestation of the olive tree especially by Bactrocera oleae is a serious economic problem. In this study, we have isolated 115 bacterial strains from various ecological niches, and tested their ability to protect the olive fruits against Bactrocera oleae. Among these strains, culture supernatant (CS) of one bacterial strain displayed the highest rate of larval mortality, and was identified as Bacillus subtilis by 16S rRNA molecular analysis. Further characterization of the CS of the Bacillus sp. strain showed that the highest insecticidal activity against third instar larvae occurs at pH 7. Our results indicate that this bacteria strain may be a prospective alternative in pest control programs.

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Characterisation of three mosquitocidal Bacillus strains isolated from mangrove forest

Cited by 30 publications

References 22 publications

RETRACTED ARTICLE: Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India

RETRACTED ARTICLE: Mosquitocidal properties of Bacillus species isolated from mangroves of Vellar estuary, Southeast coast of India

Binding Sites of Mosquitocidal Toxins ofPseudomonas FluorescensandBacillus Subtilison Pupae and Larvae ofCulex quinquefasciatus

Biocontrol potential of a Bacillus subtilis strain against Bactrocera oleae

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