Characterisation of the subtelomeric regions of Giardia lamblia genome isolate WBC6

Prabhu, Anjali; Morrison, Hilary G.; Martínez, Charles R.; Adam, Rodney D.

doi:10.1016/j.ijpara.2006.12.011

Cited by 25 publications

(27 citation statements)

References 36 publications

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“…An exception is the hypervariable subtelomeric regions (as shown in Fig. 5) where sequences can vary within the same isolate and chromosome (Upcroft and Upcroft 1999;Prabhu et al 2007). A significant characteristic difference between WB and 106 is the 1.5-Mb chromosome 3/4 doublet in 106, whereas no such doublet is apparent in WB ).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the PFOR1 gene (clone G6/1 in the work of ) and C3/19 also belong to the same linkage group on chromosome 3/4 ( Fig. 2 Adam et al 1988;Yu et al 2002;Prabhu et al 2007) was mapped using NotI-cleaved DNA with NotI segments identified by chromosome 6-specific probes and the GiardiaDB. The positions and identity (letters F, A, etc.)…”

Section: Deletions From Ch991763mentioning

confidence: 99%

“…However, confirmation that specific contigs are indeed associated with others on certain scaffolds ultimately depends on outcomes of laboratory bench work. Meanwhile, there is no consensus regarding which scaffolds are assigned to the five major G. duodenalis chromosomes of parasites of assemblage A, one of two so-called assemblages which infect man (Monis et al 1996), other than a handful of markers previously published including the PFOR1 gene sequence Upcroft and Upcroft 1999;Adam et al 1988;Yu et al 2002;Prabhu et al 2007). Indeed, the number, sizes, and naming of assemblage A G. duodenalis chromosomes as shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

“…The estimated sizes of chromosome 3/4 (C3/4), chromosome 5 (C5), and chromosome C6 are in comparison with yeast chromosome markers (BioRad) as shown in the marker (M) lane. Chromosome designation I-V as reported originally by Adam et al (1988) and later 1-5 (Yu et al 2002;Prabhu et al 2007) is indicated far right (Chen et al 1994). The map was constructed using complete NotI cleavages of G. duodenalis DNA.…”

Section: Introductionmentioning

confidence: 99%

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Sequence map of the 3-Mb Giardia duodenalis assemblage A chromosome

et al. 2009

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The genome of the gut protozoan parasite Giardia duodenalis (assemblage A) has been sequenced and compiled as contigs and scaffolds (GiardiaDB- http://GiardiaDB.org ), but specific chromosome location of all scaffolds is unknown. To determine which scaffolds belong to the 3-Mb chromosome, a library of probes specific for this chromosome was constructed. The probes were hybridised to NotI-cleaved whole chromosomes, and the combined size of different NotI segments identified by the probes was 2,225 kb indicating the probes were well distributed along the 3-Mb chromosome. Six scaffolds (CH991814, CH991779, CH991793, CH991763, CH991764, and CH991761) were identified as belonging to the 3-Mb chromosome, and these scaffolds were ordered and oriented according to scaffold features including I-PpoI sites and hybridisation pattern. However, the combined size of scaffolds was more than 4 Mb. Approximately, 1 Mb of scaffold CH991763 carrying previously identified sequences specific for the 1.5-Mb chromosome(s) including subtelomeric sequence was reassigned, and several other anomalies were addressed such that the final size of the apparently 3-Mb chromosome is estimated to be 2,885 kb. This work addresses erroneous computer-based assignment of a number of contigs and emphasises the need for alternative and confirmatory methods of scaffold construction.

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Section: Discussionmentioning

confidence: 99%

Section: Deletions From Ch991763mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sequence map of the 3-Mb Giardia duodenalis assemblage A chromosome

et al. 2009

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“…1995; Prabhu et al. 2007) and extremely small but with typical chromatin condensation levels (Tumova et al. 2015).…”

Section: Introductionmentioning

confidence: 99%

Constitutive aneuploidy and genomic instability in the single‐celled eukaryote Giardia intestinalis

et al. 2016

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Giardia intestinalis is an important single‐celled human pathogen. Interestingly, this organism has two equal‐sized transcriptionally active nuclei, each considered diploid. By evaluating condensed chromosome numbers and visualizing homologous chromosomes by fluorescent in situ hybridization, we determined that the Giardia cells are constitutively aneuploid. We observed karyotype inter‐and intra‐population heterogeneity in eight cell lines from two clinical isolates, suggesting constant karyotype evolution during in vitro cultivation. High levels of chromosomal instability and frequent mitotic missegregations observed in four cell lines correlated with a proliferative disadvantage and growth retardation. Other cell lines, although derived from the same clinical isolate, revealed a stable yet aneuploid karyotype. We suggest that both chromatid missegregations and structural rearrangements contribute to shaping the Giardia genome, leading to whole‐chromosome aneuploidy, unequal gene distribution, and a genomic divergence of the two nuclei within one cell. Aneuploidy in Giardia is further propagated without p53‐mediated cell cycle arrest and might have been a key mechanism in generating the genetic diversity of this human pathogen.

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Subtelomeres

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Characterisation of the subtelomeric regions of Giardia lamblia genome isolate WBC6

Cited by 25 publications

References 36 publications

Sequence map of the 3-Mb Giardia duodenalis assemblage A chromosome

Sequence map of the 3-Mb Giardia duodenalis assemblage A chromosome

Constitutive aneuploidy and genomic instability in the single‐celled eukaryote Giardia intestinalis

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