Characterisation of the Roundup Ready soybean insert

Abstract

In this article we describe the isolation and characterisation of the junction between insert DNA and plant DNA in the transgenic Roundup Ready soybean line event 40-3-2. Our results establish that during integration of the insert DNA several rearrangements occurred at the 3' NOS junction and that the genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the characterisation of junction regions is, in our opinion, the method of choice to support method development for detection and identification of plant biotechnology-derived products

Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method

“…A range of genome walking strategies such as anchored PCR, ligation-mediated PCR and inverse PCR have been used to identify junction sequences and develop event specific tests for a number of transgenic cultivars including Roundup-Ready soybean (Windels et al 2001), Mon810 MaisGard maize (Holck et al 2002), Bt11 maize , Starlink maize (Windels et al 2003), Mon863 maize (Yang et al 2005b), NewLeaf potato (Cote et al 2005), T25 maize (Collonnier et al 2005), Bt11, Bt176, GA21 maize and GT73 canola (Taverniers et al 2005). An event specific detection method for Mon1445 has also been described (Yang et al 2005a).…”

Detection of Genetically Modified Organisms in Foods by DNA Amplification Techniques

“…However, due to its broad specificity these methods do not allow for the unequivocal identification of discrete transformation events. The more common strategy pursues the amplification of 10 sequences found in as many different GMOs as possible, e.g. some promoters and terminators used in most of the first GMOs approved for commercialisation.…”

“…These are unique for each transformation event and result from the combination of sequences in the transgenic construct and those at the insertion point at the genome of the host. 10,[32][33][34] It is interesting to remind that, under the EU Regulations, the subject of the authorisations for commercialisation is a specific transformation event, any other transgenic variety obtained with the same construct should go throughout all the authorisation process.…”

“…antibiotic resistance or herbicide resistance when this is not the primary character, signalling peptides for fusion proteins, or introns. [7][8][9][10] In the case of transgenic plants, the GMO resulting from the stable insertion of a specific construction in the genome is known as a rmation event. It is characterised by the insertion, in a precise chromosomal location, of one or several copies, either complete or truncated, of the construct.…”

“…In some instances, the insertion of the construct is associated with rearrangements of the plant genome. 10 Genetic engineering is used in agriculture and food industries in order to improve the performance of plant varieties (resistance to plagues, herbicides, and hydric or saline stresses), improve technological properties during storage and processing (firmness of 6 fruits), or improve the sensorial and nutritional properties of food products (starch quality, content on vitamins or essential amino acids). [11][12][13][14] …”