Characterisation of the role of an alkaline protease from Vibrio midae SY9 in enhancing the growth rate of cultured abalone fed a probiotic-supplemented feed

Huddy, Robert J.; Coyne, Vernon E.

doi:10.1016/j.aquaculture.2015.05.048

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…Probiotics effectively participate in the digestive process by producing extracellular enzymes such as proteases, carbohydrolases and lipases, and by providing growth factors (Arllano and Olmos ; Ochoa and Olmos ). Vibrio midae SY9 enhanced digestive protease activity, protein digestion and absorption levels, and growth rate of Haliotis midae (Huddy and Coyne ). Photosynthetic bacteria and Bacillus spp.…”

Section: Modes Of Actionsmentioning

confidence: 99%

The use of probiotics in aquaculture

2015

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Summary This study aims to present comprehensive notes for the use of probiotics in aquaculture. Probiotics have been proven to be positive promoters of aquatic animal growth, survival and health. In aquaculture, intestines, gills, the skin mucus of aquatic animals, and habitats or even culture collections and commercial products, can be sources for acquiring appropriate probiotics, which have been identified as bacteria (Gram‐positive and Gram‐negative) and nonbacteria (bacteriophages, microalgae and yeasts). While a bacterium is a pathogen to one aquatic animal, it can bring benefits to another fish species; a screening process plays a significant role in making a probiotic species specific. The administration of probiotics varies from oral/water routine to feed additives, of which the latter is commonly used in aquaculture. Probiotic applications can be either mono or multiple strains, or even in combination with prebiotic, immunostimulants such as synbiotics and synbiotism, and in live or dead forms. Encapsulating probiotics with live feed is a suitable approach to convey probiotics to aquatic animals. Dosage and duration of time are significant factors in providing desired results. Several modes of actions of probiotics are presented, while some others are not fully understood. Suggestions for further studies on the effects of probiotics in aquaculture are proposed.

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Section: Modes Of Actionsmentioning

confidence: 99%

The use of probiotics in aquaculture

2015

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“…For instance, Aeromonas media was found to increase the tolerance of Pacific oysters to disease caused by V. tubiashii [33]. Moreover, the growth of South African abalone increased when fed with a diet supplemented with V. midae SY9, a beneficial species of Vibrio [34].…”

Section: Discussionmentioning

confidence: 99%

Effects of Salinity on Acid Production and Growth of Three Probiotic Microbes with Potential for Application in Intensive Shrimp Aquaculture

Pinoargote¹,

Ravishankar²

2018

J Prob Health

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Shrimp has been among the top value-added products targeted for production by the aquaculture industry. The increasing demand for shrimp has led to a massive increase in production in several countries across the world. Intensive and super-intensive production systems are facing great challenges handling newly emerging shrimp diseases. The use of antibiotics was one of the first approaches when dealing with such diseases, but the effects of misusing antibiotics and the appearance of antibiotic resistant bacteria are of public concern. As an alternative, probiotics have been applied in aquaculture systems to increase disease resistance, improve feed efficiency, maintain water quality and enhance the growth of aquatic organisms. In this study, the ability of three probiotic microbes to tolerate salinity levels commonly found in intensive shrimp production systems were evaluated. Lactobacillus casei, Saccharomyces cerevisiae and Rhodopseudomonas palustris were cultured in MRS broth, yeast and mold broth, and Van Neil's broth, respectively, enriched with 1 and 2% NaCl. Microbial survival between treatments were compared as well as the metabolic activity in terms of acidity levels. Additionally, cell morphology was compared using scanning electron microscopy. L. casei and S. cerevisiae showed no significant differences (P>0.05) in media with 1% and 2% NaCl in terms of microbial survival and media acidity levels at 24 h. R. palustris showed a prolonged lag phase extending up to 12 h in 1% and 48 h in 2% NaCl media, and acidity of the media did not vary significantly. Cell morphology of all microbes did not change significantly across all treatments. From these results, it was concluded that L. casei, S. cerevisiae and R. palustris are suitable for application in aquaculture ponds with up to 2% salinity.

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“…It casts some light on the feeding ecology, trophic relationships and diet preferences of organisms in their natural environment (Gisbert et al, 2018). Huddy and Coyne (2015) clarified that gut bacteria can improve digestion efficiency and growth parameters of the host by synthesizing extracellular enzymes that subsidize the repertoire of digestive enzymes necessary for the digestibility of complex substrates. Moreover, Hai (2015) delved into the utilization of probiotics that confer benefits to the host through advancing growth, survival and general health.…”

Section: Introductionmentioning

confidence: 99%

Effects of putative dietary probiotics from the gut of milkfish ( Chanos chanos ) on the growth performance and intestinal enzymatic activities of juvenile Nile tilapia ( Oreochromis niloticus )

Hortillosa

Amar

Nuñal

et al. 2021

Aquaculture Research

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Enzymes synthesized by the gut microbiome are recognized as vital for assisting host metabolism. This study was conducted to screen and identify bacterial isolates with enzyme-producing abilities from the intestine of milkfish Chanos chanos.Overall, 114 isolates were collected, of which 58% showed clear zones indicating various enzyme activities via plate-based assays. Specifically, 65 isolates were amylolytic, 1 isolate was cellulolytic, and 18 isolates exhibited both activities. The two most promising isolates-CC8 and CC27-were further subjected to spectrophotometric enzyme assays to determine their amylase, cellulase and protease activities. Results showed that amylase, cellulase and protease activities (6.00 ± 0.01, 0.52 ± 0.02 and 18.62 ± 0.16 U mg −1 protein, respectively) were higher in CC8 than in CC27. Using 16S rRNA gene sequencing, the isolates were putatively identified as Vibrio sp. and Bacillus cereus respectively. At a suspension (mL) to feed (g) ratio of 3:25, dietary supplementation of putative probiotics was carried out to evaluate the growth performance, survival and enzymatic activities of juvenile Nile tilapia Oreochromis niloticus. Results revealed that probiotic-supplemented fish exhibited better growth performance-final average body weight, weight gain, specific growth rate and feed conversion ratio-along with increased intestinal enzymatic activities compared with the control (p < 0.05). Survival rates ranged from 80% to 90% and were statistically similar in all treatments (p > 0.05). This study concludes that the application of Vibrio sp. CC8 and Bacillus cereus CC27, supplemented either in monostrain or multistrain preparation, can promote growth and enzymatic activities in juvenile Nile tilapia.

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Characterisation of the role of an alkaline protease from Vibrio midae SY9 in enhancing the growth rate of cultured abalone fed a probiotic-supplemented feed

Cited by 8 publications

References 51 publications

The use of probiotics in aquaculture

The use of probiotics in aquaculture

Effects of Salinity on Acid Production and Growth of Three Probiotic Microbes with Potential for Application in Intensive Shrimp Aquaculture

Effects of putative dietary probiotics from the gut of milkfish ( Chanos chanos ) on the growth performance and intestinal enzymatic activities of juvenile Nile tilapia ( Oreochromis niloticus )

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