Characterisation of polymeric UF membranes by liquid–liquid displacement porosimetry

Peinador, René Israel; Calvo, José Ignacio Madalena; Prádanos, Pedro; Palacio, Laura; Hernández, Antonio

doi:10.1016/j.memsci.2009.11.008

Cited by 44 publications

(22 citation statements)

References 28 publications

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“…However, in an earlier paper [25], it has been shown that EP can be readily adapted to characterize hollow fiber membranes, which are widely employed in MF applications. It should also be noted that wetting liquids can cause some degree of membrane swelling when the volatile wetting liquid is allowed to remain in contact with the membrane for a prolonged time [26,[33][34][35], which can skew the results of the PSD due to the increase in the pore volume and hence surface area of the membrane pores. However, because the Anopore membranes studied here are composed of aluminum oxide, membrane swelling does not occur when they are immersed in IPA or 1-butanol.…”

Section: Methodsmentioning

confidence: 99%

Extending the uppermost pore diameter measureable via Evapoporometry

Zamani

Jayaraman

Akhondi

et al. 2017

Journal of Membrane Science

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Section: Methodsmentioning

confidence: 99%

Extending the uppermost pore diameter measureable via Evapoporometry

Zamani

Jayaraman

Akhondi

et al. 2017

Journal of Membrane Science

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“…Relevant hydrodynamic properties of the two detergents are summarized in Table I. Precut composite regenerated cellulose membranes (Ultracel PLGC0251 membranes, Millipore Corp., Billerica, MA) with nominal molecular weight cut-off (MWCO) of 10 kDa and 30 kDa, corresponding to pore sizes of 2.2 AE 0.1 nm and 4.3 AE 0.8 nm (Peinador et al, 2010), respectively, were used for the experiments. The Ultracel membranes are highly hydrophilic and have very low fouling during protein ultrafiltration (van Reis and Zydney, 2007).…”

Section: Stirred-cell Ultrafiltration Experimentsmentioning

confidence: 99%

Concentrating membrane proteins using ultrafiltration without concentrating detergents

Feroz

Vandervelden

Ikwuagwu

et al. 2016

Biotech & Bioengineering

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Membrane proteins (MPs) are of rapidly growing interest in the design of pharmaceutical products, novel sensors, and synthetic membranes. Ultrafiltration (UF) using commercially available centrifugal concentrators is typically employed for laboratory-scale concentration of low-yield MPs, but its use is accompanied by a concomitant increase in concentration of detergent micelles. We present a detailed analysis of the hydrodynamic processes that control detergent passage during ultrafiltration of MPs and propose methods to optimize detergent passage during protein concentration in larger-scale membrane processes. Experiments were conducted using nonionic detergents, octyl-β-D glucoside (OG), and decyl-β-D maltoside (DM) with the bacterial water channel protein, Aquaporin Z (AqpZ) and the light driven chloride pump, halorhodopsin (HR), respectively. The observed sieving coefficient (So ), a measure of detergent passage, was evaluated in both stirred cell and centrifugal systems. So for DM and OG increased with increasing filtrate flux and decreasing shear rates in the stirred cell, that is, with increasing concentration polarization (CP). Similar effects were observed during filtration of MP-detergent (MPD) micelles. However, lower transmission was observed in the centrifugal system for both detergent and MPD systems. This is attributed to free convection-induced shear and hence reduced CP along the membrane surface during centrifugal UF. Thus to concentrate MPs without retention of detergent, design of UF systems that promote CP is required. Biotechnol. Bioeng. 2016;113: 2122-2130. © 2016 Wiley Periodicals, Inc.

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“…The porosimeter used in the analysis consists in an automated device developed in the SMAP laboratories in the University of Valladolid [15,19]. A detailed description of the equipment and the experimental procedure can be seen elsewhere [15].…”

Section: Liquid-liquid Displacement Porosimetrymentioning

confidence: 99%

“…This equipment can be used to obtain important information on the structure of UF membranes, including pore size distribution and porosity [14][15][16][17][18][19]. This equipment has been used in this work to characterize several virus retentive membranes, at a research stage, and the information obtained is correlated and compared with results from other virus membranes characterization methods, to give an idea on how LLDP can be of help to membrane manufacturers and end-users for an appropriate selection of membrane characteristics.…”

Section: Introductionmentioning

confidence: 99%