Characterisation of plasmids harbouring extended-spectrum cephalosporin resistance genes in Escherichia coli from French rivers

Baron, Sandrine; Devendec, Laëtitia Le; Lucas, Pierrick; Larvor, Emeline; Jové, Thomas; Kempf, Isabelle

doi:10.1016/j.vetmic.2020.108619

Cited by 14 publications

(13 citation statements)

References 35 publications

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“…These resistances (MDR, PDR and XDR) in isolates circulating in the cities of Burkina Faso could be due by the expression of resistance genes of the bla and mecA types carried by mobile and transferable genetic elements between cells of the same or different species described previously by several authors [11,13,14,37,38]. The transfer of resistance genes between isolates depends on the type of ecosystem and the mode of transfer [39][40][41][42][43].…”

Section: Discussionmentioning

confidence: 99%

Spread and antibiotic resistance profile of pathogens isolated from human and hospital wastewater in Ouagadougou

Abasse¹,

Boukaré²,

Sampo³

et al. 2021

Microbes and Infectious Diseases

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Background:The discharge of improperly treated hospital landfill presents an enormous public health risk. In Burkina Faso, the management of hospital's wastewaters and the current antibiotic susceptibility of clinically relevant isolates need to be determined, because the multi-drug resistant isolates have been previously described in hospital settings. The aim of this study was to determine antibiotic resistance profile of isolates circulating in Ouagadougou. Methods: The biochemical characterization of the isolates was carried out by tests from the API 20E test and completed with molecular characterization by simple polyemerase chain reaction (PCR). Antibiotic susceptibility of the isolates was determined using the recommendations of CA-SFM 2019. Results: The hospital wastewaters do not undergo any treatment before been discharged into the environment. A total of 171 presumed isolates of Salmonella spp, Pseudomonas spp, and Escherichia coli were identified in this study. These isolates derived from environment (n=19) and clinical (n=152). These isolates were resistant to amoxicillin + clavulanic acid (95.32%), cefoxitin (72.51%), ceftazidime (78.94%), cefepime (80.71%), tobramycin (59.64%), gentamicin (42.10%), nalidixic acid (68.42%), norfloxacin (59.06%), ciprofloxacin (56.14%), imipenem (0.00%), chloramphenicol (26.31%), and colistin (77.77%). Somewhere else, 46 presumptive Staphylococcus aureus were resistant to vancomycin (30.43%), oxacillin (13.04%), penicillin G (89.13%), ceftriaxone (15.21%), cefoxitin (2.73%),t (36.95%), kanamycin (30.43%), ciprofloxacin (15.21%), norfloxacin (43.47%), Tetracycline (56.52%), chloramphenicol (13.04%), and Fosfomycin (2.73%). Conclusion: The hospital wastewaters harbour a variety of pathogens, most of which are resistant to several families of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Spread and antibiotic resistance profile of pathogens isolated from human and hospital wastewater in Ouagadougou

Abasse¹,

Boukaré²,

Sampo³

et al. 2021

Microbes and Infectious Diseases

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“…coli ‐producing CTX‐M‐15, CTX‐M‐55, CTX‐M‐14, CTX‐M‐3, CTX‐M‐65, CTX‐M‐9, CTX‐M‐27, CTX‐M‐125, and CTX‐M‐2. The predominant vehicles of fosA3 are IncFII plasmids including F2:A‐:B‐, F33:A1:B1, and F33:A‐:B‐ (Baron et al, 2020; Benzerara et al, 2017a; Lupo et al, 2018; Mendes et al, 2016). IncF plasmids are limited by host range to the family of Enterobacteriaceae and are usually low copy number plasmids >100 kb in size (Carattoli, 2013).…”

Section: Plasmid Types Involved In the Dissemination Of Fosfomycin‐momentioning

confidence: 99%

“…Of the 21 randomly selected isolates, one plasmid (IncF33:A‐:B‐) contained the resistance determinants bla CTX‐M‐55 , bla TEM‐1b‐like, and fosA and was highly similar to a plasmid from an E . coli of chicken origin in China (Baron et al, 2020).…”

Section: Mobile Fosfomycin Resistance Genes In Enterobacteriaceae Isomentioning

confidence: 99%

“…The efficacy of fosfomycin against extended‐spectrum beta‐lactamase (ESBL)‐producing Enterobacteriaceae is favorable although increasing fosfomycin resistance rates due to fosfomycin‐modifying enzymes have been observed among ESBL‐producing and carbapenemase‐producing Enterobacteriaceae in the last decade, primarily in Asia but more and more globally (Baron et al, 2020; Benzerara et al, 2017; Chan et al, 2014; Fernandes et al, 2018; Ho, Chan, Lo, Lai, et al, 2013; Hou et al, 2012; Wachino et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Mobile fosfomycin resistance genes in Enterobacteriaceae—An increasing threat

et al. 2020

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Fosfomycin, originally known as phosphonomycin, was discovered five decades ago and has been approved for the treatment of uncomplicated urinary tract infections (UTI) since early 1970 (Silver, 2017). The phosphonic acid-derived antibiotic containing an epoxide and a propyl group possesses a unique chemical structure and the mechanism of action comprising broad-spectrum activity against Gram-negative and Gram-positive aerobic bacteria is unrelated to any other antibiotic family (Castañeda-García et al., 2013; Silver,

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“…The bla CTX – M –1 /IncI1-Iγ/ST3 plasmid is epidemiologically relevant, as it has been recovered in E. coli isolates from numerous animal and human sources in several countries in Europe, Asia and North America ( Zurfluh et al, 2015 ; Baron et al, 2020 ; Moffat et al, 2020 ), indicating its significant role in the worldwide spread of bla CTX – M –1 genes ( Carattoli et al, 2021 ). Furthermore, bla CTX – M –1 /IncI1-Iγ/ST3 plasmids quasi similar to those found in E. coli from Guadeloupe (reference pEC405) were described in E. coli isolates from France recovered from four river samples and two chicken fecal samples, demonstrating its fitness, which contributes to its persistence in E. coli communities and its geographical spread.…”

Section: Discussionmentioning

confidence: 99%

High Prevalence of blaCTXM–1/IncI1-Iγ/ST3 Plasmids in Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates Collected From Domestic Animals in Guadeloupe (French West Indies)

et al. 2022

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Extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) have been classified in the group of resistant bacteria of highest priority. We determined the prevalence of ESBL-E collected in feces from household and shelter pets in Guadeloupe (French West Indies). A single rectal swab was taken from 125 dogs and 60 cats between June and September 2019. The prevalence of fecal carriage of ESBL-E was 7.6% (14/185, 95% CI: 4.2-12.4), within the range observed worldwide. The only risk factor associated with a higher prevalence of ESBL-E rectal carriage was a stay in a shelter, suggesting that refuges could be hotspots for their acquisition. All but one (Klebsiella pneumoniae from a cat) were Escherichia coli. We noted the presence of a blaCTX–M–1/IncI1-Iγ/sequence type (ST3) plasmid in 11 ESBL-producing E. coli isolates belonging to ST328 (n = 6), ST155 (n = 4) and ST953 (n = 1). A blaCTX–M–15 gene was identified in the three remaining ESBL-E isolates. The blaCTX–M–1 and most of the antimicrobial resistance genes were present in a well-conserved large conjugative IncI1-Iγ/ST3 plasmid characterized by two accessory regions containing antibiotic resistance genes. The plasmid has been detected worldwide in E. coli isolates from humans and several animal species, such as food-producing animals, wild birds and pets, and from the environment. This study shows the potential role of pets as a reservoir of antimicrobial-resistant bacteria or genes for humans and underlines the importance of basic hygiene measures by owners of companion animals.

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Characterisation of plasmids harbouring extended-spectrum cephalosporin resistance genes in Escherichia coli from French rivers

Cited by 14 publications

References 35 publications

Spread and antibiotic resistance profile of pathogens isolated from human and hospital wastewater in Ouagadougou

Spread and antibiotic resistance profile of pathogens isolated from human and hospital wastewater in Ouagadougou

Mobile fosfomycin resistance genes in Enterobacteriaceae—An increasing threat

High Prevalence of blaCTXM–1/IncI1-Iγ/ST3 Plasmids in Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates Collected From Domestic Animals in Guadeloupe (French West Indies)

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