Characterisation of isolates and strains of citrus tristeza closterovirus using restriction analysis of the coat protein gene amplified by the polymerase chain reaction

Gillings, Michael R.; Broadbent, P.; Indsto, James O.; Lee, Richard

doi:10.1016/0166-0934(93)90065-y

Cited by 87 publications

(88 citation statements)

References 10 publications

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“…RNA viruses have a great potential for genetic variation due to their error-prone RNA replication, large populations, and short replication times (Rubio et al, 2001). These enzymes were also effective in detecting polymorphism in CTV isolates in other studies (Gillings et al, 1993;Valle et al, 2000;Roy et al, 2003). According to these authors the Hinf I enzyme provides better discrimination among the isolates.…”

Section: Discussionmentioning

confidence: 79%

“…However, the use of the primers RFL33/RFL34 restricts haplotype diversity since haplotypes with mutations in the target sequence could not be amplified. This is not observed when degenerate primers are used (Gillings et al, 1993 The UPGMA analysis (Figure 1), generated by the Jaccard genetic similarity coefficient calculated from the data obtained with the digestion with Hinf I and Rsa I, showed that the Pera Vacinada and Barão B controls differed approximately 56% and 60% from the other isolates. The remaining CTV isolates were divided into two co-related large groups with a genetic coefficient of similarity of approximately 80%.…”

Section: Rflp Analysis Of Cp Nucleotide Sequencesmentioning

confidence: 99%

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Comparison of Citrus tristeza virus (CTV) isolates by RFLP analysis of the coat protein nucleotide sequences and by the severity of the symptoms

Corazza¹,

Zanutto²,

Zanineli-Ré³

et al. 2012

Trop. plant pathol.

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Plants of Pera sweet orange on Rangpur lime rootstocks, from orchards of the northwest and north of Paraná state, Brazil, were evaluated for severity of symptoms and genetic diversity of Citrus tristeza virus. The severity of symptoms was evaluated by the development of tree, fruit size and stem pitting symptoms. Isolates that infect these plants were compared with known mild and severe isolates by analysis of restriction fragments length polymorphism (RFLP) of the coat protein nucleotide sequences (CPNS), amplified by the polymerase chain reaction (PCR) and undergone digestion with the restriction enzymes Hinf I and Rsa I. The severity of symptoms showed that the analyzed plants from the northwest orchards presented mild to moderate tristeza symptoms, while the plants from the north orchards exhibited moderate to severe symptoms. The RFLP analysis revealed that the CTV isolates are constituted by haplotype mixtures. Rsa I was the enzyme that best discriminated the genetic diversity among the analyzed isolates of CTV. Two main groups were generated by the UPGMA analysis. The isolates from the northwest orchards grouped with most of the mild isolates used as control, and a great part of the isolates from the north orchards, was correlated with the severe isolate Capão Bonito. Correlation between the stem pitting intensity and RFLP patterns, was demonstrated with some exceptions. The failure of protection of some isolates and the contamination of the rootstocks by the severe isolates, in field nursery conditions, before grafting with scions with mild isolates, were the hypotheses considered to explain the occurrence of severe CTV isolates in the North area of Paraná State.

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Section: Discussionmentioning

confidence: 79%

Section: Rflp Analysis Of Cp Nucleotide Sequencesmentioning

confidence: 99%

Comparison of Citrus tristeza virus (CTV) isolates by RFLP analysis of the coat protein nucleotide sequences and by the severity of the symptoms

Corazza¹,

Zanutto²,

Zanineli-Ré³

et al. 2012

Trop. plant pathol.

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“…The HinfI restriction fragment patterns revealed that the Malaysian CTV population structure is complex. By using the HinfI restriction fragment patterns for separating mild strains from sever strains, the Group IV was identified as mild strain (Gilling et al, 1993), so the 15 isolates recognized as Group IV (AMT8, AMT15, AMT28, AMK1, AMK8, AMK17, AMK19, AMK22, AMK27, AMK42, AMC18, AMM11, AMM20, AMM28 AND AMJ12), were either mild strain or complexes of mild and severe strains. By comparing these two methods for separating mild strains from severe strains, it is perceived that BD-PCR cannot recognize all mild strains.…”

Section: Discussionmentioning

confidence: 99%

“…CTV isolates were first classified into seven p25/HinfI groups. Groups IV and V produced mild symptoms while others produced sever symptoms in the indicator plants (Gilling et al, 1993). Later another group was found in china and defined as Group VIII.…”

Section: Introductionmentioning

confidence: 99%

“…Digestion of the PCR products of CTV isolates revealed a high sequence divergence between CTV isolates (Figure 2). According to the previous defined RFLP groups (Gilling et al, 1993;Jiang et al, 2008), the restriction enzyme HinfI created digestion patterns of Groups I to IV, VI to VII and two other new groups. Isolates AMK1, AMJ12, AMT38, AMT39 and AMT43 produced a unique restriction pattern with two fragments of about 220 and 300 bp.…”

Section: Rflp Profiles Of the Cp Gene From Different Ctv Isolatesmentioning

confidence: 99%

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Molecular characterization of Citrus tristeza virus strains in Peninsular Malaysia

Ayazpour¹,

Sijam²,

Vadamalai³

et al. 2011

Afr. J. Microbiol. Res.

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Sixty Malaysian Citrus tristeza virus (CTV) isolates were characterized by bi-directional polymerase chain reaction (BD-PCR) and restriction fragment length polymorphism (RFLP) analysis of their coat protein (CP) gene. In BD-PCR analysis, 392-bp fragments were amplified from seven isolates. The other 53 isolates produced only 320-bp fragments. RFLP patterns of RT-PCR products of CP gene digested with HinfI restriction enzyme were similar to I-IV, VI-VII and two new groups. Isolates AMK1, AMJ12, AMT38, AMT39 and AMT43 could not be classified when they were compared to any standard CTV digest pattern. These isolates produced a unique restriction pattern with two fragments of 210 and 300 bp and isolate AMI61 produced different restriction pattern with three fragments of about 100, 270 and 300 bp. Therefore these isolates were designated as Groups IX and X. These results suggest that CTV populations in Malaysia contain new genetic variants.

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Diagnosis and Management of Virus and Virus like Diseases of Citrus

Roistacher

Diseases of Fruits and Vegetables Volume I

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Characterisation of isolates and strains of citrus tristeza closterovirus using restriction analysis of the coat protein gene amplified by the polymerase chain reaction

Cited by 87 publications

References 10 publications

Comparison of Citrus tristeza virus (CTV) isolates by RFLP analysis of the coat protein nucleotide sequences and by the severity of the symptoms

Comparison of Citrus tristeza virus (CTV) isolates by RFLP analysis of the coat protein nucleotide sequences and by the severity of the symptoms

Molecular characterization of Citrus tristeza virus strains in Peninsular Malaysia

Diagnosis and Management of Virus and Virus like Diseases of Citrus

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