Characterisation of <i>staphylococcus aureus</i> bacteraemia at Tygerberg hospital

Orth, Heidi; Dreyer, Zubeida Salaam; Makgotlho, Edward; Oosthuysen, Wilhelm F.; Sinha, Bhanu; Wasserman, E

doi:10.1080/10158782.2013.11441515

Cited by 7 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These were previously reported as dominant in these provinces (6). The spa-type CC 012 in these two provinces was similar to a study from Tygerberg Academic Hospital (16). When the six most common spa-types (t037, t1257, t012, t045, t064 and t032) and SCCmec types were correlated, SCCmec types II, III, IV and V and all six spa-types were linked to hospital-associated infections.…”

Section: Discussionsupporting

confidence: 80%

A small proportion of community-associated methicillin-resistant Staphylococcus aureus bacteraemia, compared to healthcare-associated cases, in two South African provinces

Perovic

Singh-Moodley

Govender

et al. 2017

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

We compared the proportion of cases of community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus (CA-MRSA and HA-MRSA, respectively) bacteraemia among patients at five hospitals in the Gauteng and Western Cape provinces in South Africa and described the molecular characteristics and antimicrobial susceptibility trends. This was a cross-sectional study using data collected by enhanced surveillance for S. aureus bacteraemia. A total of 2511 cases of S. aureus bacteraemia were identified from January 2013 to January 2016. Among 1914 cases of S. aureus, 557 (29.1%) cases were identified as MRSA infection. Forty-four cases (44/1914 [2.3%] of all S. aureus cases) were considered CA-MRSA infection and 513/1914 (26.8% of all cases) had HA-MRSA infection; the majority were neonates. CA-MRSA constituted 7.9% (44/557) of all cases of MRSA infection. Staphylococcus aureus isolates demonstrated significantly reduced susceptibility to the following classes of antimicrobial agents: macrolides, tetracyclines, aminoglycosides and cotrimoxazole, in 2015 compared to 2013 (p < 0.05). Of the 557 MRSA isolates, 484 (87%) were typed for SCCmec elements and spa types: the most common SCCmec type was type III (n = 236, 48.76%), followed by type IV (n = 144, 29.76%). The most common spa types were t037 (n = 229, 47.31%) and t1257 (n = 90, 18.60%). Of 28 isolates selected for multilocus sequence typing (MLST), the most common sequence types (STs) were ST239 and ST612 of clonal complex 8 (CC8) (n = 8 each) and a novel ST (ST4121) was obtained for one isolate. This study demonstrates that S. aureus bacteraemia is common in South African academic centres and characterised by HA-MRSA SCCmec types III and IV. A small proportion of CA-MRSA cases were caused by a few different sequence types.

show abstract

Section: Discussionsupporting

confidence: 80%

A small proportion of community-associated methicillin-resistant Staphylococcus aureus bacteraemia, compared to healthcare-associated cases, in two South African provinces

Perovic

Singh-Moodley

Govender

et al. 2017

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…Additionally, spa-typing revealed 47 different spa-types with the five most common spatypes (t037, t1257, t045, t064 and t012) accounting for 87% and the most common ST was ST612 clonal complex (CC8) (n = 7) followed by ST5 (CC5) (n = 4), ST36 (CC30) (n = 4) and ST239 (CC8) (n = 3). Findings from this study correlated with previous studies in South Africa [25][26][27]. This study demonstrated the importance of monitoring molecular typing trends to detect changing epidemiological trends in antimicrobial resistance patterns [24].…”

Section: Molecular Diagnostics and Typing In South African Surveillansupporting

confidence: 87%

Molecular diagnostics in South Africa and challenges in the establishment of a molecular laboratory in developing countries

Singh-Moodley¹,

Ismail²,

Perovic³

2020

J Infect Dev Ctries

View full text Add to dashboard Cite

The laboratory plays a significant role in public health surveillance, outbreak investigation and infection prevention and control strategies. Microbiology laboratories are moving towards incorporating molecular biology techniques for the surveillance and identification of pathogens causing infectious diseases as well as the genotypic characterisation of these organisms. These methods are accurate, rapid, reliable, and provide a wealth of information that are not available using conventional phenotypic methods. However, establishing such a laboratory can be challenging in developing countries due to poor infrastructure, the lack of funding and the required expertise. This manuscript discusses the essential issues that need to be addressed when establishing a molecular microbiology laboratory and the usefulness of molecular techniques in public health surveillance and outbreaks in developing countries. Molecular data on South African findings obtained from surveillance and outbreak studies are also presented in this manuscript.

show abstract

“…Certain pandemic MRSA clones are spread worldwide. Recent publications describe the ST612-MRSA-IV clone (MLST CC8) as the most widespread MRSA clone in Cape Town, and South Africa (Jansen van Rensburg et al, 2011;Orth et al, 2013;Oosthuysen et al, 2013;Moodley et al, 2010). In this study ST 612 formed strong biofilms, which may contribute to its dominance in nosocomial infections in South Africa.…”

Section: Discussionmentioning

confidence: 69%

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Naicker

Karayem

Hoek

et al. 2016

Microbial Pathogenesis

View full text Add to dashboard Cite

2 Declaration I, Preneshni Rochelle Naicker, hereby declare that the work contained in this assignment is my original work and that I have not previously submitted it, in its entirety or in part, at any university for a degree. The ability to form biofilms contributes significantly to the virulence of Staphylococcus aureus. Virulence factors may be associated with certain S. aureus lineages. However, the contribution of the genetic background of S. aureus to biofilm formation is poorly understood. This study investigated the association between the genetic background and the biofilm forming ability of clinical invasive S. aureus isolates. Secondary objectives included investigating any correlation with biofilm formation and methicillin resistance or the source of bacteraemia. Methods:The study was conducted at a 1300-bed tertiary hospital in Cape Town, South Africa. S. aureus isolates obtained from blood cultures between January 2010 and January 2012 were included. Genotypic characterization was performed by PFGE, spa typing, SCCmec typing and MLST. Thirty genotypically unique strains were assessed for phenotypic biofilm formation with the microtitre plate assay. All isolates were tested in triplicate and an average optical density, measured at a wavelength of 490nm, was determined. The biofilm forming ability of isolates with A 490 > 0.17 was considered 'weak positive' and A 490 > 0.34 'strong positive'. Isolates with A 490 ≤ 0.17 were considered non-adherent. ANOVA with Bonferroni-adjusted post-hoc tests and t-tests were used for statistical analysis of the association between biofilm forming ability and strain characteristics.Results: Fifty seven percent of isolates were capable of forming biofilms. Weak biofilm formation occurred in 40% (n=12) and strong biofilm formation in 17% (n=5) of isolates. Thirteen (43%) of the isolates were classified as non-adherent. All 5 isolates capable of strong biofilm formation belong to one spa clonal complex (spa-CC 064). Strains from spa-CC 064 were capable of higher biofilm formation than other spa clonal complexes (p=0.00002). These 5 strains belonged to MLST CC5 and CC8. Biofilm formation did not correlate with methicillin resistance and was not related to the source of bacteraemia. Conclusion:Biofilm formation correlates with the spa clonal lineage in our population of invasive S. aureus strains. High biofilm formation was associated with spa-CC 064. MLST CC5 and CC8 strains are capable of strong biofilm formation.

show abstract

Characterisation of staphylococcus aureus bacteraemia at Tygerberg hospital

Cited by 7 publications

References 10 publications

A small proportion of community-associated methicillin-resistant Staphylococcus aureus bacteraemia, compared to healthcare-associated cases, in two South African provinces

A small proportion of community-associated methicillin-resistant Staphylococcus aureus bacteraemia, compared to healthcare-associated cases, in two South African provinces

Molecular diagnostics in South Africa and challenges in the establishment of a molecular laboratory in developing countries

Biofilm formation in invasive Staphylococcus aureus isolates is associated with the clonal lineage

Contact Info

Product

Resources

About