Characterisation of <i>Phaseolus vulgaris</i> Cultivars by their electrophoretic patterns

Adriaanse, A.; Klop, W.; Robbers, J. E.

doi:10.1002/jsfa.2740201104

Cited by 34 publications

(11 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Isozymes and TP banding patterns have been used earlier to identify species (Bingham and Yeh 1971;Ladizinsky 1975) and cultivars (Adriaanse et al 1969). In Napier grass, acid phosphatase (ACP) and two esterases (ESTs) produced 13 and 15 bands, respectively in 58 germplasm accessions and grouped them into 2-3 clusters (Augustin and Tcacenco 1993).…”

Section: Discussionmentioning

confidence: 99%

Application of Isozyme Data in Fingerprinting Napier Grass (Pennisetum purpureum Schum.) for Germplasm Management

Bhandari

Sukanya

Ramesh

2006

Genet Resour Crop Evol

View full text Add to dashboard Cite

Sixty-four accessions of Napier grass (Pennisetum purpureum Schum.), a forage crop of tropics and subtropics, were assessed for polymorphism in isozymes and total proteins (TP). The national germplasm collection exhibited polymorphism in all the three isozymes viz., malate dehydrogenase, glutamate oxaloacetate transaminase and peroxidase (POX) besides TP profile. Though POX and TP showed comparatively higher polymorphism, the study indicated the efficiency of three-enzyme system in identifying more than 90% of the accessions. The combination of TP and three-enzyme systems provided unique isozyme/TP banding patterns for all the 64 accessions leading to development of accession specific profile/fingerprints. In Napier, which is perennial with long vegetative phase, it is difficult to discriminate germplasm accessions based only on morphology. Thus biochemical markers could be efficiently utilized to complement morphological evaluations, in maintaining identity and purity of germplasm for proper conservation and management, for better use in breeding and for proprietary reasons.

show abstract

Section: Discussionmentioning

confidence: 99%

Application of Isozyme Data in Fingerprinting Napier Grass (Pennisetum purpureum Schum.) for Germplasm Management

Bhandari

Sukanya

Ramesh

2006

Genet Resour Crop Evol

View full text Add to dashboard Cite

show abstract

“…Seed protein markers are quite stable, uniform, quick, reliable, reproducible and seldom affected by environmental fluctuations and management practices (Dunhill and Fowden 1965;Adriaanse et al 1969;Gray et al 1973;Basha 1979;Naik 1998). The stability, uniformity and additive nature of seed protein profile derived through sodium-dodecyl-sulphate polyacrylamide gel electrophoresis (SDS-PAGE) has effectively been employed in studies on evolution, domestication, genotype characterization and genetic diversity assessment in wild and domesticated plant populations of several crop plants including grain legumes (Ladizinsky and Hymowitz 1979;Osborn 1988;Gepts 1990;Ahmed and Slinkard 1992;Samour 1989).…”

Section: Introductionmentioning

confidence: 99%

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Panigrahi¹,

Kumar²,

Mishra³

et al. 2007

Plant Biotechnol Rep

View full text Add to dashboard Cite

SDS-PAGE analysis of seed albumins and globulins from two pigeonpea, Cajanus cajan, cultivars (DSLR-17 and BDN-2) and ten wild species, including C. cajanifolius, C. lineatus, C. sericeus, C. acutifolius, C. lanceolatus, C. reticulates, C. albicans, C. scarabaeoides, C. volubilis and C. platycarpus, resulted in 34 albumin and 27 globulin polypeptides. Proximity matrix analysis based on electrophoretic banding patterns of albumins and globulins jointly revealed C. cajanifolius to be closest to C. cajan having similarity coefficients of 0.595 and 0.676, respectively. Cluster analysis also exhibited the grouping of C. cajanifolius with C. cajan in one cluster. C. platycarpus has been out grouped. The present analysis more or less agreed with the sectional classification of the genus Cajanus and it has been hypothesized that cultivated pigeonpea has evolved through multi-genomic interaction involving C. cajanifolius and that it has experienced minor genomic reorganization during its divergence.

show abstract

“…Electrophoresis of seed proteins has previously been used to identify beans of different colour classes (Adriaanse et al, 1969;Hussain et al, 1986). The present study has shown that it is possible to achieve good differentiation within a colour class and among samples that are indistinguishable based on seed morphology, by using selected electrophoretic methods.…”

Section: Discussionmentioning

confidence: 99%

Isoenzyme and cotyledon protein variation for identification of black beans (Phaseolus vulgaris L.) with similar seed morphology

et al. 1993

View full text Add to dashboard Cite

Methods developed to identify genetically diverse varieties of beans (Phaseolus vulgaris L.) were applied to closely related lines that were difficult to distinguish on the basis of seed morphology. Seedling tissues and seeds of black beans, were examined electrophoretically for isoenzyme and cotyledon protein patterns. Seven enzymes, extracted from seeds or from seedling stem, root or leaf tissues, were compared for polymorphism. Peptidase, polyphenol oxidase, phosphoglucoisomerase and glutamate oxaloacetate transaminase patterns were the same for all lines. Some differences were observed for acid phosphatase, peroxidase and esterase patterns, but complete discrimination of the six selected lines was not possible on the basis of isoenzyme patterns alone. Using polyacrylamide gel electrophoresis (PAGE) of dissociated 0.

show abstract

Characterisation of Phaseolus vulgaris Cultivars by their electrophoretic patterns

Cited by 34 publications

References 9 publications

Application of Isozyme Data in Fingerprinting Napier Grass (Pennisetum purpureum Schum.) for Germplasm Management

Application of Isozyme Data in Fingerprinting Napier Grass (Pennisetum purpureum Schum.) for Germplasm Management

Genomic relationships among 11 species in the genus Cajanus as revealed by seed protein (albumin and globulin) polymorphisms

Isoenzyme and cotyledon protein variation for identification of black beans (Phaseolus vulgaris L.) with similar seed morphology

Contact Info

Product

Resources

About