Characterisation of<i>Medicago truncatula</i>CLE34 and CLE35 in nodulation control

Mens, Céline; Hastwell, April H.; Su, Huanan; Gresshoff, Peter M.; Mathesius, Ulrike; Ferguson, Brett J.

doi:10.1101/2020.07.31.231605

Cited by 4 publications

(1 citation statement)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CLE peptides whose gene expression is induced (+) or repressed (−) during mycorrhization (AM), nodulation (Nod), high phosphate treatment (P) or nitrate treatments (Karlo et al 2020; Lebedeva et al 2020; Mens et al 2020; Mortier et al 2010; Müller et al 2019) are indicated between brackets. Those CLE peptides with a reported function in the autoregulation of nodulation (AON) or autoregulation of mycorrhization (AOM) (Imin et al 2018; Lebedeva et al 2020; Mens et al 2020; Mortier et al 2010; Müller et al 2019) are also indicated, and all of them belong to the same clade. Sl CLE10 and Sl CLE11 peptides, that are functionally characterized in this study, are labelled with a red arrow.…”

Section: Resultsmentioning

confidence: 99%

CLE11 and CLE10 Suppress Mycorrhizal Colonisation in Tomato

Wulf

Wang

Plagaro

et al. 2023

Preprint

View full text Add to dashboard Cite

Symbioses with beneficial microbes are widespread in plants, but these relationships must balance the energy invested by the plants with the nutrients acquired. Symbiosis with arbuscular mycorrhizal (AM) fungi occurs throughout land plants but our understanding of the genes and signals that regulate colonisation levels is limited. Here, we demonstrate that in tomato two CLV3/EMBRYO-SURROUNDING REGION (CLE) peptides, SlCLE10 and SlCLE11, act to suppress AM colonisation of roots. Mutant studies and overexpression via hairy transformation indicate SlCLE11 acts locally in the root to limit AM colonisation. Indeed, SlCLE11 expression is strongly induced in AM colonised roots but SlCLE11 is not required for phosphate suppression of AM colonisation. SlCLE11 may act through as yet uncharacterised signalling pathways, as SlCLE11 does not suppress AM colonisation by acting through two previously characterised receptors with roles in regulating AM colonisation, SlFAB (CLAVATA1 orthologue) or SlCLV2. SlCLE10 appears to play a more minor or redundant role, as cle10 mutants did not influence AM, although the fact that ectopic overexpression of SlCLE10 did suppress colonisation suggests SlCLE10 may play a role in regulating AM colonisation. Our findings show that CLE peptides regulate AM colonisation in the non-legume species tomato.

show abstract

Section: Resultsmentioning

confidence: 99%

CLE11 and CLE10 Suppress Mycorrhizal Colonisation in Tomato

Wulf

Wang

Plagaro

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Autoregulation dependent and independent mechanisms are responsible for the systemic control of nodule formation by the plant N demand

Pervent

Lambert

Tauzin

et al. 2021

Preprint

View full text Add to dashboard Cite

In legumes interacting with rhizobia the formation of symbiotic organs responsible for the acquisition of atmospheric nitrogen is depending of the plant nitrogen (N) demand. We discriminated between local and systemic impact of nitrogen on nodule formation using Medicago truncatula plants cultivated in split-root systems. We obtained evidence of the control of nodule formation by whole plant systemic N-satisfaction signaling but obtained little evidence of a local control by mineral nitrogen. We characterized the impact of systemic N signaling on the root transcriptome reprogramming associated to nodule formation. We identified, large genes clusters displaying common expression profiles in response to systemic N signaling enriched in particular fonctions required during these biological processes. We found evidence of a strong effect of SUNN in the control by systemic N signaling of many genes involved in the early interaction with rhizobium as well as organogenesis supporting a role of autoregulation pathway in systemic N signaling. However, we also found evidence that major SUNN independent systemic N signaling controls were maintained in the mutant. This study shed light on the unexpected high complexity of the control of nodule formation by systemic N signaling, that probably involves multiple pathways.

show abstract

The Regulation of Nodule Number in Legumes Is a Balance of Three Signal Transduction Pathways

Chaulagain

Frugoli

2021

IJMS

View full text Add to dashboard Cite

Nitrogen is a major determinant of plant growth and productivity and the ability of legumes to form a symbiotic relationship with nitrogen-fixing rhizobia bacteria allows legumes to exploit nitrogen-poor niches in the biosphere. But hosting nitrogen-fixing bacteria comes with a metabolic cost, and the process requires regulation. The symbiosis is regulated through three signal transduction pathways: in response to available nitrogen, at the initiation of contact between the organisms, and during the development of the nodules that will host the rhizobia. Here we provide an overview of our knowledge of how the three signaling pathways operate in space and time, and what we know about the cross-talk between symbiotic signaling for nodule initiation and organogenesis, nitrate dependent signaling, and autoregulation of nodulation. Identification of common components and points of intersection suggest directions for research on the fine-tuning of the plant’s response to rhizobia.

show abstract

Characterisation ofMedicago truncatulaCLE34 and CLE35 in nodulation control

Cited by 4 publications

References 64 publications

CLE11 and CLE10 Suppress Mycorrhizal Colonisation in Tomato

CLE11 and CLE10 Suppress Mycorrhizal Colonisation in Tomato

Autoregulation dependent and independent mechanisms are responsible for the systemic control of nodule formation by the plant N demand

The Regulation of Nodule Number in Legumes Is a Balance of Three Signal Transduction Pathways

Contact Info

Product

Resources

About