Characterisation of <i>Brucella</i> species and biovars in South Africa between 2008 and 2018 using laboratory diagnostic data

Matle, Itumeleng; Ledwaba, Betty; Madiba, None Karabelo; Makhado, Lavhelesani; Jambwa, Kudakwashe; Ntushelo, Nombasa

doi:10.1002/vms3.483

Cited by 13 publications

(6 citation statements)

References 43 publications

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“…A number of bacterial agents of abortion are contagious and/or become endemic in herds. Brucella abortus ( B. abortus ) is common and Brucella melitensis ( B. melitensis ) as well as Brucella ovis ( B. ovis ) are isolated sporadically in South Africa (Kolo et al 2019 ; Matle et al 2021 ; Mbizeni 2015 ). Campylobacter fetus ssp.…”

Section: Introductionmentioning

confidence: 99%

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Jonker

Thompson

Michel

2023

Onderstepoort Journal of Veterinary Research

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Abortions in domestic ruminants cause significant economic losses to farmers. Determining the cause of an abortion is important for control efforts, but it can be challenging. All available diagnostic methods in the bacteriology laboratory should be employed in every case due to the many limiting factors (autolysis, lack of history, range of samples) that complicate the investigation process. The purpose of this study was to determine whether the recovery of diagnostically significant isolates from domestic ruminant abortion cases could be increased through the use of a combination of the existing aerobic culture and Brucella selective method with methods that are commonly recommended in the literature reporting abortion investigations. These methods are examination of wet preparations and impression smears stained by the modified Ziehl–Neelsen method, anaerobic, microaerophilic, Leptospira, Mycoplasma and fungal culture. Samples of placenta and aborted foetuses from 135 routine clinical abortion cases of cattle (n = 88), sheep (n = 25) and goats (n = 22) were analysed by the new combination of methods. In 46 cases, bacteria were identified as aetiological agents and in one case a fungus. Isolation of Brucella species increased to 7.4% over two years compared with the previous 10 years (7.3%), as well as Campylobacter jejuni (n = 2) and Rhizopus species (n = 1). Salmonella species (5.9%) and Trueperella pyogenes (4.4%) were also isolated more often. In conclusion, the approach was effective in removing test selection bias in the bacteriology laboratory. The importance of performing an in-depth study on the products of abortion by means of an extensive, combination of conventional culture methods was emphasised by increased isolation of Brucella abortus and isolation of C. jejuni. The combination of methods that yielded the most clinically relevant isolates was aerobic, microaerophilic, Brucella and fungal cultures.

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Section: Introductionmentioning

confidence: 99%

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Jonker

Thompson

Michel

2023

Onderstepoort Journal of Veterinary Research

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“…MLVA11 identified B. abortus bv 3 isolated from cattle as new major clusters from WA. In contrast to WA where B. abortus biovar 3 strains occur in cattle, B. abortus biovar 1 is the dominate biovar in South Africa ( 46 ). These data suggest that the predominated B. abortus biovars are unique in WA compared to southern Africa, but further investigation is needed to better understand the epidemiological trait of B. abortus in Africa.…”

Section: Discussionmentioning

confidence: 99%

Original and introduced lineages co-driving the persistence of Brucella abortus circulating in West Africa

Liu

Wang

Shi

et al. 2023

Front. Public Health

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IntroductionBrucellosis, a serious public health issue affecting animals and humans, is neglected in West Africa (WA).MethodsIn the present study, bio-typing, multi-locus sequence typing (MLST), multiple-locus variable-number tandem repeat analysis (MLVA), and whole genome sequencing single-nucleotide polymorphism (WGS-SNP) analysis were used to characterize the Brucella abortus (B. abortus) strains from WA.ResultsAll of the 309 strains analyzed in this study were extracted and downloaded from the international MLVA bank and were from 10 hosts (cattle, humans, ovine, buffalo, dromedaries, horse, sheep, zebu, dog, and cat) distributed in 17 countries in WA. Based on the bio-typing, three biovars, dominated by B. abortus bv.3, were observed and reported across seven decades (1958–2019). With MLST, 129 B. abortus strains from the present study were sorted into 14 STs, with ST34 as the predicted founder. These 14 STs clustered into the global MLST data into three clone complexes (C I–C III) with the majority of strains clustering in C I, while C II forms an independent branch, and C III harbors three STs shared by different continents. These data revealed that most cases were caused by strains from native lineages. According to the MLVA-11 comparison, 309 strains were divided into 22 MLVA-11 genotypes, 15 of which were unique to WA and the remaining seven had a global distribution. MLVA-16 analysis showed that there were no epidemiological links among these strains. Based on the MLVA data, B. abortus strains from WA have high genetic diversity, and predominated genotypes were descended from a native lineage. While the MLVA-16 globally highlights that the dominant native and few introduced lineages (from Brazil, the USA, South Korea, Argentina, India, Italy, Portugal, the UK, Costa Rica, and China) co-driving the B. abortus ongoing prevalence in WA. The high-resolution SNP analysis implied the existence of introduced B. abortus lineages, which may be reasonably explained by the movement and trade of dominant hosts (cattle) and/or their products.DiscussionOur results indicated that B. abortus strains in WA consist of native and introduced strains that necessitate control such as vaccination, testing, slaughtering, and movement control by the relevant country authorities to reduce brucellosis in livestock.

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“…Although, despite of its endemicity in developing countries, many of the developed nations have strategically control the spread of brucellosis but in past 3 years it has been reported as a most re-current, re-emerging, intermittent infection in Japan, China, India, Australia and other European Countries [6]. Progressive risk of contamination occurs with close contact to infected animals or through generated aerosols, continuous bacterial shedding by underlying tissues, raw under-cooked meat, food articles of animal origin and spontaneously through incomplete processing of milk and its by-products [7]. Disease symptoms of typical flu-like allergy begins with acute illness and later on progresses with neurological involvement of central and peripheral system in chronic derivatives [8].…”

Section: Introductionmentioning

confidence: 99%

Comparative Surface Interaction Study to Detect Brucella melitensis 16M Using Biosensor Transducer Modifications with 4-MBA, ZnONPs/AuNPs, ZnONPs/AuNPs/4-MBA

Hans¹,

Thavaselvam²

2023

austinjbiosensbioelectron

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Brucellosis is a zoonotic disease endemic in developing countries and caused by gram-negative bacteria of genus Brucella infecting both livestock and humans. Brucella melitensis and Brucella abortus are important species representing largest biotypes world-wide. Therefore, modified detection strategies and advancement in potential analytical tools are required to monitor its rapid prevalence. In this study, we aim to modify gold-transducer of Surface Plasmon Resonance biosensor with combination of metal oxide nanomaterials and chemical probe to detect recombinant outer membrane ‘rOmp28’ protein antigen of Brucella melitensis 16M in concentration-dependent surface interactions. We synthesized Zinc (ZnONPs) and Gold (AuNPs) nanoparticles using standard ‘Hydrothermal and Turkevich Methods’ and their crystalline structure, chemical property and morphology were analysed using UV-Visible Spectrophotometry, FT-IR, Powder-XRD, SEM-EDX and TEM-SAED. For immobilizing specific rOmp28 derived IgG-pAbs on modified Au-transducer, rOmp28 protein was expressed and purified using Ni-NTA gel affinity chromatography for producing pAbs in BALB/c mice. Three modifications of Au-transducer with 4-MBA, ZnONPs/AuNPs and ZnONPs/AuNPs/4-MBA were subjected for immobilization and SPR biosensing was performed with rOmp28 Ag at detection range of 0.1 μg mL-1 to 0.01 fg mL-1. Limit of detection observed with ZnONPs/AuNPs/4-MBA combination was 0.1 fg mL-1 by relative increase in SPR response angle at 0.1 μg mL-1 in the order 83.7° < 98.9° < 179.2° for 4-MBA < ZnONPs/AuNPs < ZnONPs/AuNPs/4-MBA respectively. In conclusion, metal oxide nanomaterials in combination with biosensor are suitable in sensitive and specific interaction of antigen displaying lowest LODs and enhanced biosensor response for on-field real-time Brucella detection in both clinical and non-clinical disease scenario.

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Characterisation of Brucella species and biovars in South Africa between 2008 and 2018 using laboratory diagnostic data

Abstract: The genus Brucella consists of pathogenic members that have zoonotic potential and are of veterinary and economic importance (Moreno, 2014). The pathogenic members of the genus Brucella cause a disease known as brucellosis in a wide variety of domes-

Cited by 13 publications

References 43 publications

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Approaches to increase recovery of bacterial and fungal abortion agents in domestic ruminants

Original and introduced lineages co-driving the persistence of Brucella abortus circulating in West Africa

Comparative Surface Interaction Study to Detect Brucella melitensis 16M Using Biosensor Transducer Modifications with 4-MBA, ZnONPs/AuNPs, ZnONPs/AuNPs/4-MBA

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