Characterisation of five alcohol dehydrogenases from Lactobacillus reuteri DSM20016

Hu, Zhenghong; Jia, Ping; Bai, Yajun; Fan, Tai‐Ping; Zheng, Xiaohui; Cai, Yujie

doi:10.1016/j.procbio.2019.08.010

Cited by 14 publications

(7 citation statements)

References 30 publications

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“…The genes in the Lysine biosynthesis pathway mainly inhibit the synthesis of redox-related enzymes and hydrolases ( Table 4 ). Alcohol dehydrogenase are a group of oxidoreductases which encoded by lp_0862 gene during the stage of formation ( Hu, Pu & Bai, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells

Yan

Liu

et al. 2020

PeerJ

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Background Lactiplantibacillus plantarum (L. plantarum), a dominant strain in traditional fermented foods, is widely used in fermentation industry because of its fast acid production. However, L. plantarum is easily inactivated due to acidity, high temperature and other factors. The formation of biofilm by bacteria can effectively increase environmental tolerance. Therefore, it is important to improve the environmental tolerance of L. plantarum by studying its biofilm formation conditions and regulatory mechanisms. Methods After determining a suitable NaCl concentration for promoting biofilm formation, L. plantarum was grown with 48 g L−1 NaCl. Differential gene expressions in L. plantarum biofilm vs. planktonic cells were analyzed using RNA sequencing and validated using qPCR. Result L. plantarum RS66CD biofilm formation formed highest amount of when grown at 48 g L−1 NaCl. Altogether 447 genes were up-regulated and 426 genes were down-regulated in the biofilm. KEGG pathway analysis showed that genes coding for D-Alanine metabolism, peptidoglycan biosynthesis, two-component system, carbon metabolism, bacterial secretion system, lysine biosynthesis and fatty acid metabolism were crucial for biofilm formation. In addition, eight other genes related to biofilm formation were differentially expressed. Our results provide insights into the differential gene expression involved in biofilm formation, which can help to reveal gene regulation during L. plantarum biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells

Yan

Liu

et al. 2020

PeerJ

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“…Few studies characterised purified ADHs of LAB. In cheese, Hu et al (2019) found five ADHs of Limosilactobacillus reuteri DSM20016 which could reduce aldehydes into their corresponding alcohols, for instance, 3-methylbutanal to 3-methylbutanol, butyraldehyde to 2-butanol, hexanal to hexanol, phenylacetaldehyde to phenylethanol, etc.…”

Section: Introductionmentioning

confidence: 99%

Screening of lactic acid bacteria for their potential use as aromatic starters in fermented vegetables

Lorn

Nguyen²,

et al. 2021

International Journal of Food Microbiology

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“…Cells were lysed by sonication in an ice bath for 15 min with 30% power, and the supernatant was obtained by centrifugation at 10 000 × g for 10 min under 4 °C. The recombinant CASDHs were purified and desalinated using a 5 mL Ni‐HisTrap column and HisTrap desalting column (GE Healthcare, Shanghai, China) 28 . The crude enzymes and purified CASDHs were verified by 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‐PAGE).…”

Section: Methodsmentioning

confidence: 99%

“…The recombinant CASDHs were purified and desalinated using a 5 mL Ni-HisTrap column and HisTrap desalting column (GE Healthcare, Shanghai, China). 28 The crude enzymes and purified CASDHs were verified by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The protein concentrations were quantified by Bradford method; 29 a BCA protein assay kit (Beyotime Biotechnology, China) was used.…”

Section: Bacterial Strains Plasmids and Growth Conditionsmentioning

confidence: 99%

Overexpression and biochemical characterization of a carboxyspermidine dehydrogenase from Agrobacterium fabrum str. C58 and its application to carboxyspermidine production

et al. 2022

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BACKGROUND Carboxyspermidine (C‐Spd) is a potentially valuable polyamine carboxylate compound and an excellent building block for spermidine synthesis, which is a critical polyamine with significant implications for human health and longevity. C‐Spd can also be used to prepare multivalent cationic lipids and modify nucleoside probes. Because of these positive effects on human health, C‐Spd is of considerable interest as a food additive and pharmaceutical target. RESULTS A putative gene afcasdh from Agrobacterium fabrum str. C58, encoding carboxyspermidine dehydrogenase with C‐Spd biosynthesis activity, was synthesized and transformed into Escherichia coli BL21 (DE3) for overexpression. The recombinant AfCASDH was purified and fully characterized. The optimum temperature and pH for the recombinant enzyme were 30 °C and 7.5, respectively. The coupled catalytic strategy of AfCASDH and various NADPH regeneration systems were developed to enhance the efficient production of C‐Spd compound. Finally, the maximum titer of C‐Spd production successfully achieved 1.82 mmol L−1 with a yield of 91% by optimizing the catalytic conditions. CONCLUSION A novel AfCASDH from A. fabrum str. C58 was characterized that could catalyze the formation of C‐Spd from putrescine and l‐aspartate‐β‐semialdehyde (L‐Asa). A whole‐cell catalytic strategy coupled with NADPH regeneration was established successfully for C‐Spd biosynthesis for the first time. The coupled system indicated that AfCASDH might provide a feasible method for the industrial production of C‐Spd. © 2021 Society of Chemical Industry.

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Characterisation of five alcohol dehydrogenases from Lactobacillus reuteri DSM20016

Cited by 14 publications

References 30 publications

Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells

Comparative transcriptomic analysis of Lactiplantibacillus plantarum RS66CD biofilm in high-salt conditions and planktonic cells

Screening of lactic acid bacteria for their potential use as aromatic starters in fermented vegetables

Overexpression and biochemical characterization of a carboxyspermidine dehydrogenase from Agrobacterium fabrum str. C58 and its application to carboxyspermidine production

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