Characterisation of downy mildew resistant and susceptible pearl millet (<i>Pennisetum glaucum</i>(L.) R.Br.) genotypes using isozyme, protein, randomly amplified polymorphic DNA and inter-simple sequence repeat markers

Mahatma, M. K.; Bhatnagar, Rakesh; Solanki, R. K.; Mittal, Gaurav; Shah, R. R.

doi:10.1080/03235408.2011.559038

Cited by 3 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study indicated that the wilt resistant genotypes (ICP 8863, Vaishali and ICPL 87119) clustered in same linkage group whereas wilt susceptible genotypes (ICP 2376 and T 15-15 ) clustered into another linkage group. Similar results were observed in wilt resistant and susceptible genotypes of chickpea by Ratnaparkhe et al (1998) and in downy mildew resistant and susceptible genotypes of pearl millet by Mahatma et al (2011).…”

Section: Resultssupporting

confidence: 84%

Molecular characterization of wilt resistant and susceptible genotypes of <italic>Cajanus cajan</italic> (L.)

Swami

Mahatma

Parekh

et al. 2015

View full text Add to dashboard Cite

Characterization of germplasm is important for breeding programme. Therefore, in present investigation 9 genotypes of pigeonpea [<italic>Cajanuscajan</italic> (L) Millsp] comprising three wilt resistant genotypes (Vaishali, ICPL 87119 and national check ICP 8863) and two susceptible genotypes (T<sub>15-15</sub> and national check ICP 2376) along with four other popular genotypes grown in Gujarat, India (BDN-2, GT-100, GT-102 and C-11) were characterized using RAPD, ISSR and SSR markers. Genetic diversity of 9 pigeonpea genotypes revealed an average polymorphism of 39.18% by RAPD, 47.67 % by ISSR and 57.35 % by SSR. On the basis of polymorphism (%), PIC(polymorphic information content) value, MI (Marker index) value primer OPI-12 was found most informative among 12 RAPD primers. While ISSR primer UBC-850 was found most informative among 8 ISSR primers showing 80% polymorphism, 0.51 PIC value and 40.80 MI value. SSR primers gave 2 to 5 amplicon on a 6 % polyacrylamide gel. The average PIC value was 0.36, ranging from 0 to 0.73. PIC also provides the estimate of discriminatory power of a locus in the case of SSR marker system by taking into account not only the number of alleles expressed, but also the relative frequency of those alleles. Our prime target of discrimination between wilt resistant and susceptible genotypes of pigeon pea was achieved by ISSR and SSR markers. However, SSR was more efficient in generation of polymorphism among the genotypes based on the information on the level of polymorphism and the PIC value.

show abstract

Section: Resultssupporting

confidence: 84%