Characterisation of deactivating agents and their influence on the stability of manganese‐dependent peroxidase from Phanerochaete chrysosporium

Abstract

Production of manganese-dependent peroxidase (MnP) by Phanerochaete chrysosporium in semi-solid-state cultures is described. The stability of MnP during the production stage and in the crude enzyme preparations was studied. Inactivation of MnP by various factors including proteases, hydrogen peroxide and pH value in semi-solid-state cultures on an inert support (nylon sponge) was studied. Strategies to avoid inactivation are described. Hydrogen peroxide was shown to be essential for enzyme deactivation. A method for the elimination of H 2 O 2 based on catalase addition has been designed which resulted in improvement in both enzyme production and stability.

Role of Laccase and Low Molecular Weight Metabolites fromTrametes versicolorin Dye Decolorization

“…However, considering that this effect was not clearly produced after catalase addition and the decolorization level attained after 1 day or more time was increased in comparison with experiment without catalase, the enzymatic stabilization hypothesis appears to be the most possible one. This result is in agreement with the stabilization effect of catalase observed with other ligninolytic enzymes [ 22 ]. Two completely different decolorization processes seems to coexist: decolorization by peroxides with possible generation of radicals, and enzymatic decolorization.…”

Color removal ability of Phanerochaete chrysosporium in relation to lignin peroxidase and manganese peroxidase produced in molasses wastewater

“…Phanerochaete chrysosporium is the one of the well-studied fungi in this respect and its enzymatic systems catalyse degradation of lignin and lignin-like materials during the secondary phase of the metabolic growth (Eriksson et al 1990). Synthesis and secretion of lignin peroxidase or ligninase (LiP) and manganese-dependent peroxidase (MnP) are triggered by nutrient limitations such as source of carbon and/or nitrogen (Keyser et al 1978;Kirk et al 1978;Jeffries et al 1985;Cabalerio et al 2001). This study was undertaken to test the decolorizing ability of P. chrysosporium in relation to lignin peroxidase and manganese peroxidase produced in MWW in the course of decolorization of this effluent.…”

“…Although most of the organic matter of this type of effluents is removed by means of conventional biodegradation treatments, the dark colour due to the presence of melanoidin-type compounds with high molecular weight, is little eliminated by these processes. White-rot fungi have a complex extracellular enzymatic system, capable of catalysing degradation of numerous recalcitrant organic compounds often present in industrial wastewater such as molasses wastewater (MWW), and which present pollution problems (Cabalerio et al 2001;Fu & Viraghavan 2001;Lacina et al 2003). Phanerochaete chrysosporium is the one of the well-studied fungi in this respect and its enzymatic systems catalyse degradation of lignin and lignin-like materials during the secondary phase of the metabolic growth (Eriksson et al 1990).…”

The effect of trace elements on wheat straw degradation by Trametes gibbosa

“…Furthermore, trace elements could modulate lignin degradation process not only through ligninolytic enzyme synthesis but also by the regulation of other factors affecting biodegradation, such as proteolytic enzymes and hydrogen peroxide concentration (Cabaleiro et al, 2001;Palmieri et al, 2001). The highest lignin degradation level noted in samples containing Mn 2þ in concentration of 1.0 mM where peak of laccase activity and high peroxidase activities were observed is indicating important role of both enzymes which act simultaneously.…”