Color removal ability of Phanerochaete chrysosporium in relation to lignin peroxidase and manganese peroxidase produced in molasses wastewater

Abstract

Decolorization of molasses wastewater (MWW) from an ethanolic fermentation plant by Phanerochaete chrysosporium was studied. By diluting MWW properly (10%v/v) and incubating it with an appropriate concentration of the spores (2.5 · 10 6 /ml), extensive decolorization occurred (75%) on day 5 of the incubation. The colour removal ability was found to be correlated to the activity of ligninolytic enzyme system: lignin peroxidase (LiP) activity was 185 U/l while manganese peroxidase (MnP) activity equaled 25 U/l. Effects of some selected operating variables were studied: manganese(II), veratryl alcohol (VA), glucose as a carbon source and urea and ammonium nitrate, each as a source of nitrogen. Results showed that the colour reduction and LiP activity were highest (76% and 186 U/l, respectively) either when no Mn(II) was added or added at the lowest level tested (0.16 mg/l to provide 0.3 mg/l). Activity of MnP was highest (25 U/l) when Mn(II) added to the diluted MWW at the highest level (100 ppm) while activity of LiP was lowest (7.1 U/l) at this level of added Mn(II). The colour reduction in the presence of the added VA was shown to be little less than in its absence (70 vs. 75%). When urea as an organic source of nitrogen for the fungus, was added to the MWW, the decolorizing activity of P. chrysosporium decreased significantly (15 vs. 75%) and no activities were detected for LiP and MnP. Use of ammonium nitrate as an inorganic source of nitrogen did not show such a decelerating effects, although no improvements in the metabolic behavior of the fungus (i.e., LiP and MnP activities) deaccelerating was observed. Effects of addition of glucose was also discussed.

Recent developments in biodegradation of industrial pollutants by white rot fungi and their enzyme system

“…Although most of the organic matter of MWW is removed by means of conventional biodegradation treatments, the removal of dark color due to the presence of melanoidin-type high molecular weight compounds is only marginal (Vahabzadeh et al 2004). WRF are however, capable of catalyzing degradation of numerous recalcitrant organic compounds often present in MWW (Fu and Viraraghavan 2001;Lacina et al 2003).…”

“…WRF are however, capable of catalyzing degradation of numerous recalcitrant organic compounds often present in MWW (Fu and Viraraghavan 2001;Lacina et al 2003). The color removal ability of Phanerochaete chrysosporium is correlated to the activity of ligninolytic enzymes LiP and MnP (Vahabzadeh et al 2004). The color reduction in the presence of VA is lower than in its absence, thus confirming the major role of MnP.…”

Phenolic removal in olive oil mill wastewater using loofah-immobilized Phanerochaete chrysosporium

“…Basal mineral salts (BS) solution contained the following compounds per liter of distilled water (Vahabzadeh et al 2004): basal medium: KH 2 PO 4 , 0.2 g; CaCl 2 , 0.01 g; MgSO 4 .7H 2 O, 0.05 g; thiamine solution 1 ml (the thiamine solution with concentration of 1 mg/ml was used) and mineral solution 1 ml. Mineral solution contained following (per liter of distilled water) MgSO 4 .7H 2 O, 3 g; MnSO 4 .…”

Effect of transport phenomena of Cladosporium cladosporioides on decolorization and chemical oxygen demand of distillery spent wash

“…Although most of the organic compounds of this type of effluent are removed by means of conventional biodegradation treatments, the dark color due to the presence of melanoidin-type compound remains as the problem (Vahabzadeh et al 2004). During the fermentation process, fungal morphology is an important parameter which influences the physical properties of fermentation broth.…”