Characterisation of Cyclosporine-Induced Suppressor Cells in Murine Delayed-Type Hypersensitivity Responses

Mirisklavos, A; Mottram, Patricia L.; Dumble, Lynette J.; Clunie, G. J. A.

doi:10.1159/000233974

Cited by 1 publication

(5 citation statements)

References 19 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This UV dose was selected in order to minimize the inflammatory effects of UV irradiation. The finding of prolongation of heart but not of skin allograft survival by UV irradiation ofthe graft recipient is in agreement with our previous studies (11,12) and those of others (13) which have found survivai of primarily vascularized grafts to be more readily prolonged than that of skin grafts in immunosuppressed recipients. This phenomenon is incompletely understood, but factors such as host vascularization of skin grafts in contrast to primarily vascularized cardiac grafts, and site of grafting may trigger different mechanisms of graft rejection.…”

Section: Discussionsupporting

confidence: 93%

“…In other experiments, using footpad swelling measurements, DTH to C3H/He cells was not suppressed by UV-induced C57/B16-specific suppressor cells (data not shown). These data were comparable with previously published findings of antigen-specific suppressor T cells in cyclosporine-treated animals (11). Suppressive activity was removed by cytotoxic treatment of the cells before transfer with anti-Thyl-2 or anti-Lyt2 monoclonal antibodies (group 8 vs group 6, groups 17 or 18 vs group 15), but was not removed by anti-L3T4 or anti-Lyt 1 monoclonal treatment (group 9 vs group 6, group 19 vs group 15).…”

Section: Characterization Of Uv Radiation-induced Suppressors Of Dth supporting

confidence: 93%

“…Of particular interest is the finding that the antigen-specific T cells suppressing the DTH response to alloantigens are of the same phenotype in UV-irradiated animals as in cyclosporine-treated animals (11), suggesting that initiation of suppressor cell formation by different agents may result in activation of a similar common pathway(s). UV irradiation in vivo has been shown to be associated with a spleen antigen presenting cell defect, depressing the ability to initiate a positive immune response (1,3,19), whereas suppression by cyclosporine is associated with decreased production of interleukin-2 from sensitized cells (20).…”

Section: Discussionmentioning

confidence: 99%

“…In assessing DTH to alloantigens, measurement of ear swelling gave a more accurate and repeatable reading of DTH and CHS response than footpad swelling, so the data reported here are based on ear swelling measurements. We have previously used footpad swelling (10,11), ear swelling (16) and radioisotopic methods (17) to measure DTH responses. The radioisotopic method was not used here because we wished to use animals with known DTH responses as donors for the cell transfer experiments and cells cannot be transferred from radiolabelled animals.…”

Section: Effect Of Uv Irradiation On Dth Responsesmentioning

confidence: 99%

“…Reeently we found that the delayed type hypersensitivity response to alloantigens in mice given the immunosuppressant cyclosporinc was reduced to background levels and Lyt 2+ antigen-specific transferable suppressor cells were formed in these animals (10,11). Cyclosporine has been extensively used as an immunosuppressant in recipients of both experimental and clinical ailografts.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A single dose of UV radiation suppresses delayed type hypersensitivity responses to alloantigens and prolongs heart allograft survival in mice

et al. 1988

View full text Add to dashboard Cite

Summary The systemic effect of ultraviolet (UV) irradiation on delayed type hypersensitivity (DTH), contact hypersensitivity (CHS) and allograft rejection was investigated in BALB/c mice which had been exposed to a single 1 h treatment with UV radiation (27 kJ/m^) from FS40 sunlamps (60% UVB). After UV irradiation (3-5 days), mice were treated on an unirradiated site with either a subcutaneous injection of allogeneic spleen cells or a topical application of the contact scnsitizer oxazolone (OX). The DTH response to allogeneic cells and the CHS response to OX elicited 6 days afterimmunization were significantly lower in UV-treated mice than in normal mice. Spleen cells from these animals were transferred intravenously into X-irradiated (600R) recipients which were immediately challenged with antigen and the DTH or CHS response elicited was determined 24 h later. Recipients of equal numbers ofcells from sensitized and normal animals (6X10^ from each donor) exhibited positive DTH or CHS responses to the antigen used to sensitize the donor. In contrast, recipients of equal numbers ofcells from animals sensitized and UV suppressed to the same antigen showed a suppressed DTH or CHS response. This suppression was antigen-specific. Treatment ofcells from UV suppressed animals, prior to transf^er, with complement and cytotoxic anti-Lyt 2 or anti-Thy 12 monoclonal antibodies abrogated the suppressive ability of these cells, in contrast to cytotoxic treatment with anti-L3T4 or anti-Lyt 1 monoclonal antibodies which had no significant effeet. The suppressor cells therefore had the phenotype Thy 12 + , Lyt 2 + , L3T4"'. Lyt l^. In a separate series of experiments BALB/c miee, either untreated or given I h of U V irradiation 3-5 days previously, were given a heart or skin allograft from a C3H/He donor. The primarily vascularized cardiac ailografts survived significantly longer in UV-irradiated than in normal recipients (median survival time 13 daysvs 10 days, /'=0 035), but the survival of C3H/He skin ailografts was unaltered in UV mice. No identification of suppressor populations was carried out in the heart allograft recipients. These experiments provide the basis for investigations ofthe possible role of UV-induced antigen-specific suppressor cells in modulating responses to alloantigens.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Characterization Of Uv Radiation-induced Suppressors Of Dth supporting

confidence: 93%