Characterisation of CTX-M-117, a Pro174Gln variant of CTX-M-15 extended-spectrum β-lactamase, from a bovine Escherichia coli isolate

Hächler, Herbert; Kotsakis, Stathis D.; Tzouvelekis, L. S.; Geser, Nadine; Lehner, Angelika; Miriagou, Vivì; Stephan, Roger

doi:10.1016/j.ijantimicag.2012.09.011

Cited by 4 publications

(2 citation statements)

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“…Hydrolysis of imipenem (NDM-1, NMC-A, and OXA-48), cephalothin (CMY-2), or cefotaxime (CTX-M-15) was measured by UV spectrophotometry. β-Lactamase concentration in the extracts was estimated using the initial velocities and the published steady state hydrolysis constants ( 22 – 26 ) by the Michaelis-Menten equation.…”

Section: Methodsmentioning

confidence: 99%

Direct Colorimetry of Imipenem Decomposition as a Novel Cost-Effective Method for Detecting Carbapenemase-Producing Enterobacteria

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Direct Colorimetry of Imipenem Decomposition as a Novel Cost-Effective Method for Detecting Carbapenemase-Producing Enterobacteria

et al. 2022

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“…Hydrolysis of imipenem (NDM-1, NMC-A and OXA-48), cephalothin (CMY-2) or cefotaxime (CTX-M-15) was measured by UV spectrophotometry. β-Lactamase concentration in the extracts was estimated using the initial velocities and the published steady state hydrolysis constants (12)(13)(14)(15)(16) by the Michaelis-Menten equation.…”

Section: β-Lactamase Preparationsmentioning

confidence: 99%

Direct colorimetry of imipenem decomposition as a novel cost effective method for detecting carabapenamase producing bacteria

Kotsakis¹,

Lambropoulou²,

Miliotis³

et al. 2022

Preprint

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In the absence of a molecule that would collectively inhibit both metallo-β-lactamases and serine reactive carbapenemases, containment of their genes' spreading is the main weapon currently available for confronting carbepenem resistance in hospitals. Cost effective methodologies rapidly detecting carbapenemase producing enterobacteria (CPE) would facilitate such measures. Herein a low cost CPE detection method was developed that was based on the direct colorimetry of the yellow shift caused by the accumulation of diketopiperazines – products of the acid catalyzed imipenem oligomerization – induced by carbapenemase action on dense solutions of imipenem/cilastatin. The reactions were studied by spectrophotometry in the visible spectrum using preparations of β-lactamases from the four molecular classes. The effects of various buffers on reactions containing the potent carbapenemases NDM-1 and NMC-A were monitored at 405 nm. Optimal conditions were used for the analysis of cell suspensions and the assay was evaluated using 38 selected enterobacteria including 29 CPE as well as nine carbapenemase-negative strains overexpressing other β-lactamases. The development of the yellow color was specific for carbapenemase containing enzyme preparations and the maximum intensity was achieved in acidic or un-buffered conditions in the presence of zinc. When applied on bacterial cell suspensions the assay could detect CPE with 96.7 % sensitivity and 100 % specificity with results being comparable to those obtained with the CARBA NP technique. Direct colorimetry of carbapenemase-induced imipenem decomposition required minimum reagents while exhibited high accuracy in detecting CPE. Therefore it should be considered for screening purposes after further clinical evaluation.

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Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

Palmeira

Ferreira

2020

Heliyon

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Food producing animal is a global challenge in terms of antimicrobial resistance spread. Extended-spectrum betalactamase (ESBL)-producing Enterobacteriaceae are relevant opportunistic pathogens that may spread in many ecological niches of the One Health approach as human, animal and environment due to intestinal selection of antimicrobial resistant commensals in food production animals. Cattle production is a relevant ecological niche for selection of commensal bacteria with antimicrobial resistance from microbiota. Enterobacteriaceae show importance in terms of circulation of resistant-bacteria and antimicrobial resistance genes via food chain creating a resistance reservoir, setting up a threat for colonization of humans and consequent health risk. ESBL-producing Enterobacteriaceae are a threat in terms of human health responsible for life threatening outbreaks and silent enteric colonization of community populations namely the elder population. Food associated colonization is a risk difficult to handle and control. In a time of globalization of food trading, population intestinal colonization is a mirror of food production and in that sense this work aims to make a picture of ESBL-producing Enterobacteriaceae in animal production for food over the world in order to make some light in this reality of selection of resistant threats in food producing animal.

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Characterisation of CTX-M-117, a Pro174Gln variant of CTX-M-15 extended-spectrum β-lactamase, from a bovine Escherichia coli isolate

Cited by 4 publications

References 4 publications

Direct Colorimetry of Imipenem Decomposition as a Novel Cost-Effective Method for Detecting Carbapenemase-Producing Enterobacteria

Direct Colorimetry of Imipenem Decomposition as a Novel Cost-Effective Method for Detecting Carbapenemase-Producing Enterobacteria

Direct colorimetry of imipenem decomposition as a novel cost effective method for detecting carabapenamase producing bacteria

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production – a threat around the world

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