Characterisation of Cellulases and Xylanase from Trichoderma virens UKM1 and its Potential in Oil Palm Empty Fruit Bunch (OPEFB) Saccharification

Abstract

To cite this article: Ngikoh, B. et al. (2017)

Inhibitory Mechanism of Trichoderma virens ZT05 on Rhizoctonia solani

“…In addition, the secondary metabolites secreted by Trichoderma have a synergistic effect during the antagonistic process. Ngikoh et al [ 84 ] showed that during culturing of T. virens UKM1 with OPEFB substrate, Trichoderma degraded OPEFB by secreting cellulase and glucanase. The contents of cellulase and glucanase were the highest on the seventh day of culture.…”

Screening and efficacy evaluation of antagonistic fungi against Phytophthora infestans and combination with arbuscular mycorrhizal fungi for biocontrol of late blight in potato

“…virens made potato plants more susceptible to infection by P. infestans. T. virens is known for its production of hydrolytic enzymes such as xylanase, cellulase, endoglucanase, b-glucosidase, and exoglucanase (Amira et al, 2011;Ngikoh et al, 2017;Sood et al, 2020) that mediate several processes such as plant signaling and interaction, plant growth and resistance to pests and diseases, and cell wall dissolution of microbes (Druzhinina et al, 2011;Ahluwalia et al, 2015;Meng et al, 2019;Sood et al, 2020). In the present study, the culture filtrate of T. virens was obtained by growing the fungus on PDB medium, a rich source of cellulose, hemicellulose, lignin, and pectin of potato origin (Lesiecki et al, 2012).…”

Xylanase production by Trichoderma virens MLT2J2 under solid-state fermentation using corn cob as a substrate

“…being among the prolific producers of xylanases, the high enzyme production cost and poor stability are major deterrents in widening their applications in the manufacturing industry. To date, xylanases producing Trichoderma species which include Trichoderma harzianum (Pathak et al 2014;Lopez-Ramirez et al 2018), T. viride (Soliman et al 2021, Ja'afaru 2013Ishida et al 2020), T. reesei (Michelin et al 2019;Hirasawa et al 2018), T. asperellum UC1 (Ezeilo et al 2019;Dortavásquez et al 2019), T. virens (Tarayre et al 2015;El-Shishtawy et al 2015;Ngikoh et al 2017), and T. pleuroticola (Korkmaz et al 2017) have been studied. Inoculation of T. virens can degrade cellulose and hemicellulose in biomass (empty fruit bunches and palm oil mill effluent) hence speed up the composting process.…”

“…Inoculation of T. virens can degrade cellulose and hemicellulose in biomass (empty fruit bunches and palm oil mill effluent) hence speed up the composting process. Trichoderma virens has been known as a potential cellulase producer particularly endoglucanase, exoglucanase and βglucosidase extracellularly for bioconversion processes and saccharification of agriculture wastes and also has good xylanase (β-xylosidase and endo-1,4-β-D-xylanase) activity (Tarayre et al 2015;Ngikoh et al 2017).…”

“…The fungal enzymes are greatly influenced by the nature, physical factors, and chemical composition of the carbon source (Peciulyte et al 2014). The different kinds of 6531 sources and chemical composition of biomass have an apparent influence on the secreted enzymes activities in cultures of fungal (Alvira et al 2013) and previous studies reported several works on using corn cob (Soliman et al 2012;Michelin et al 2019), wheat bran and rice bran (Pathak et al 2014;El-Shishtawy et al 2015), sugarcane bagasse (Mahamud and Gomes 2012), oil palm empty fruit bunch and leaves (Ajijolakewu et al 2016;Ngikoh et al 2017;Ezeilo et al 2019) as substrates for fermentation processes to produce hemicellulases. In this study, we used corn cob due to its high hemicellulose content and rich carbon, and also nitrogen sources for the growth and stimulation natural habitat of microorganisms (Elegbede and Lateef 2018).…”