Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

He, Ningning; Yang, Xingbin; Jiao, Yong; Tian, Lingmin; Zhao, Yan

doi:10.1016/j.foodchem.2012.02.018

Cited by 132 publications

(58 citation statements)

References 30 publications

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“…But at the same tested concentrations, the scavenging effects of WFPs from wolfberry fruits against these three radicals were slightly higher than RCP-II (He, Yang, Jiao, Tian, & Zhao, 2012). The activity differential between the polysaccharides may be due to their different structure, such as monosaccharide composition, molecular weight and the content of uronic acid.…”

Section: Nmr Spectroscopy Analysismentioning

confidence: 87%

Characterization and biological activities of a novel polysaccharide isolated from raspberry ( Rubus idaeus L.) fruits

Lü

et al. 2015

Carbohydrate Polymers

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Section: Nmr Spectroscopy Analysismentioning

confidence: 87%

Characterization and biological activities of a novel polysaccharide isolated from raspberry ( Rubus idaeus L.) fruits

Lü

et al. 2015

Carbohydrate Polymers

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“…Most of its functions were reported as immunity improvement, anti-oxidation, anti-radiation, anticancer, enhancing hemopoiesis, anti-aging, and enhancing sex. The presence of various functional components like polysaccharides, flavonoids, and carotenoids in L. barbarum L. fruits is believed to be responsible for these effects (He et al 2012;Yao et al 2011). Several physiological studies have focused on polysaccharides and carotenoids; however, flavonoids have been less investigated, especially for their antioxidant activity (Amagase and Farnsworth 2011;Potterat 2010).…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Magiera

Zareba

2015

Food Anal. Methods

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Polyphenols are the important active compounds present in the fruits of Lycium barbarum L. The objectives of this study were to develop an ultra-high performance liquid chromatography with UV detection (UHPLC-UV) method for qualitative and quantitative analyses of phenolic acids and flavonoids in fruits of L. barbarum L. The isolation and enrichment of polyphenols from fruits were carried out by ultrasound-assisted extraction (UAE) in conjunction with solid-phase extraction (SPE). The analytes were separated on a Poroshell 120 EC-C18 column using a binary mobile phase composed of aqueous 0.05 % trifluoroacetic acid and acetonitrile in the gradient elution mode. Under these conditions, phenolic acids and flavonoids were separated in 11 min. The selectivity of the developed UHPLC-UV method was confirmed by comparison with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis. The validation parameters such as linearity, sensitivity, precision, and accuracy were found to be highly satisfactory. The optimized method was successfully applied to the analysis of polyphenols in fruits of L. barbarum L. Additionally, their free radicalscavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl radical (DPPH • ).

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“…The hydroxyl radical scavenging effect of LMP was measured according to the procedure of He et al (2012) with slight modifications (He et al, 2012). 1.0 mL of sample solution at different concentrations (0.25, 0.5, 1.0, 1.5, and 2.0 mg mL -1 ) was mixed with 1.0 mL of 6 mM FeSO 4 .…”

Section: Hydroxyl Radical Scavenging Assaymentioning

confidence: 99%

Antioxidant activity of polysaccharides extracted from Lentinus edodes mycelia and their protective effects on INS-1 cells

Liu¹,

Wang²,

Yu³

et al. 2016

Biosci. J.

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ABSTRACT:The disruption of the delicate balance between the generation of reactive oxygen species (ROS) and antioxidant scavenging systems can lead to many health problems. Polysaccharides extracted from fungi fruit body and myceliums have several potential health benefits. In this study, the antioxidant capacity and cell protective effects of a polysaccharide isolated from Lentinus edodes mycelia (LMP) were investigated. The antioxidant properties of LMP were screened using radical scavenging (1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion), reducing power and inhibition of lipid peroxidation assays. Intracellular reactive oxygen species (ROS), in response to H 2 O 2 , was determined by the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. Rat insulinoma (INS-1) cells apoptosis was detected using flow cytometry analysis. The results showed that LMP exhibited significantly strong radical scavenging activity. At the concentration of 2.0 mg mL -1 , DPPH radical, hydroxyl radical, and superoxide anion radical scavenging rates are 80.32 ± 2.58%, 92.56 ± 3.11% and 93.73 ± 2.82%, respectively. The reducing power and inhibition of lipid peroxidation activity of LMP were as strong as the positive control. LMP alleviated the intracellular reactive oxygen species level and inhibited islet cell apoptosis significantly. The results open perspectives for studies of LMP in the treatment of diabetes.

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Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

Cited by 132 publications

References 30 publications

Characterization and biological activities of a novel polysaccharide isolated from raspberry ( Rubus idaeus L.) fruits

Characterization and biological activities of a novel polysaccharide isolated from raspberry ( Rubus idaeus L.) fruits

Chromatographic Determination of Phenolic Acids and Flavonoids in Lycium barbarum L. and Evaluation of Antioxidant Activity

Antioxidant activity of polysaccharides extracted from Lentinus edodes mycelia and their protective effects on INS-1 cells

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