Characterisation of antimicrobial resistance-associated integrons and mismatch repair gene mutations in Salmonella serotypes

Yang, Baowei; Zheng, Jie; Brown, Eric W.; Zhao, Shaohua; Meng, Jianghong

doi:10.1016/j.ijantimicag.2008.08.016

Cited by 14 publications

(10 citation statements)

References 25 publications

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“…This study supports previous reports [19][20][21][22][23] that antimicrobial resistance in Salmonella is both a human and veterinary problem. This is further backed by reports from the USDA [24] which state that approximately 25% of small feedlot cattle operations and 70% of large feedlot operations use antimicrobials in their feed.…”

Section: Discussionsupporting

confidence: 92%

“…This is further backed by reports from the USDA [24] which state that approximately 25% of small feedlot cattle operations and 70% of large feedlot operations use antimicrobials in their feed. In ND, observed resistance against Tetracycline, Streptomycin, Sulfisoxazole, Ampicillin and Chloramphenicol was in tandem with, although slightly lower than, reports from four other state veterinary diagnostic laboratories in the US [22]. Also, Salmonella isolates from ND showed lower resistance towards Tetracycline, Streptomycin, Sulfisoxazole, Ampicillin and Chloramphenicol compared to isolates from Uganda where greatest resistance was towards Tetracycline, Streptomycin, Sulfisoxazole, Ampicillin and Chloramphenicol.…”

Section: Discussionmentioning

confidence: 59%

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Antimicrobial Resistance and Presence of Class 1 Integrons in Salmonella Serovars Isolated from Clinical Cases of Animals and Humans in North Dakota and Uganda

DK¹

2012

Clin Microbial

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Back ground: Salmonellae are one of the leading causes of food borne illness worldwide and have been used as indicator organisms for studying antimicrobial resistance (AMR) trends. In the United States, Salmonella are among organisms currently under public health surveillance for AMR. Objectives: The objective of this study was to characterise AMR patterns of Salmonella isolates from animals and humans in North Dakota (ND), and Kampala, Uganda and determine the association between the observed AMR and presence of class 1 and 2 integrons. Methods: Salmonella isolates were collected from the Veterinary Diagnostic Laboratory (VDL) at North Dakota State University and the North Dakota Department of Health, from 2003 to2008. Additional samples were also retrieved from archives at the Microbiology Department, Faculty of Veterinary Medicine at Makerere University in Kampala, Uganda. AMR profiles were determined using a panel of 15 antimicrobials. Screening for the class 1 and 2 integrons was done using PCR with primers specific for the int1 and int2. Results: Out of 359 Salmonella isolates tested 36.2% were resistant to at least 2 antimicrobials. The highest resistance frequency was seen against Tetracycline (39.6%) and Streptomycin (34.7 %). A total of 20.7% (57/276) of the ND samples tested positive for presence of class 1 integrons and was significantly associated (p<0.05) with AMR to Ampicillin, Kanamycin, Tetracycline and Sulfisoxazole. Of all Ugandan Salmonella isolates tested (94.4% 68/72) were resistant to ≥2 antimicrobials with highest resistance observed against Sulfisoxazole and Trimethoprim-Sulphamethoxazole. Presence of class 1 integron was significantly associated (p<0.05) with AMR to Tetracycline and Amoxicillin. DNA sequencing of the class 1 integron variable regions identified several resistance genes including aadA1, dfrA7, and dfrA5 genes. Conclusion: These results signal serious implications for treatment of salmonellosis in both public and animal health. of America (US), the major pathogens that have been associated with food borne outbreaks are comprised of viruses, bacteria, parasites, toxins, metals and prions [9]. Of these 7 major food pathogens (Campylobacter jejuni, Clostridium pefrigens, Escherichia coli, Listeria monocytogenes, Salmonella, Staphlococcus aureus and Toxoplasma gondii) are known to cause 3.3-12.3 million cases of food borne illness and up to 3900 deaths, with an estimated total cost of $6.5-$ 34.9 billion (1995 US$) annually [9]. Salmonella is responsible for approximately 1.4 million illnesses, 17,000 hospitalisations and 590 deaths in the US annually [9]. According to Food Net (Food borne Diseases Active Surveillance Network), Salmonella prevalence has consistently remained high in comparison to the other food borne pathogens despite various intervention measures [2]. In 2011 estimates, C li nical M ic r o b io logy : O p e n Acces s

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 59%

Antimicrobial Resistance and Presence of Class 1 Integrons in Salmonella Serovars Isolated from Clinical Cases of Animals and Humans in North Dakota and Uganda

DK¹

2012

Clin Microbial

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“…The expression of efflux pumps was not evaluated as it was beyond the scope of the present study. Mutations in gyrA among high level fluoroquinolone-resistant strains of E. coli, Salmonella, and Shigella spp., were common, whereas mutations in gyrB and parE genes were rare (Chu, Houang, & Cheng, 1998;Ling et al, 2003;Eaves et al, 2004;Cui et al, 2008;Yang, Zheng, Brown, Zhao, & Meng, 2009). The exact role of amino acid substitutions in parE is unclear.…”

Section: Discussionmentioning

confidence: 99%

Mutations in gyrase and topoisomerase genes associated with fluoroquinolone resistance in Salmonella serovars from retail meats

Yang

Cui

et al. 2012

Food Research International

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“…Acquired resistance evolves via horizontal transfer of antimicrobial resistance genes located on various types of mobile DNA elements. A key system involved in spreading antibiotic multiresistance is the integron, an element that, although normally immobile itself, can be transferred through mobile genetic elements [ 8 , 9 ]. Integrons are genetic elements that incorporate exogenous open reading frames by recombination and convert them to functional genes [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Variability in gene cassette patterns of class 1 and 2 integrons associated with multi drug resistance patterns in Staphylococcus aureus clinical isolates in Tehran-Iran

et al. 2015

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BackgroundTo investigate antibiotic resistance, the occurrence and distribution of class 1 and 2 integrons in multidrug- resistant Staphylococcus aureus isolates from hospitals in Tehran, Iran.The isolates were examined for susceptibility to antimicrobial agents. The mecA gene, class 1 and 2 integrons were detected by PCR. Integrase positive strains were further analysed for the presence of resistance gene cassettes using specific primers and were sequenced.ResultsAmong 139 S.aureus isolates, 109 (78.4 %) and 112 (80.5 %) strains were considered as multidrug resistant and mecA positive, respectively. Class 1 integrons and internal variable regions were found in 72.6 % (101/139) and 97 % (98/101) and class 2 integrons and variable regions also in 35.2 % (49/139) and 65.3 % (32/49) of S.aureus clinical isolates, respectively. Twelve distinct cassette arrays were found, containing genes encoding resistance to β-lactams, aminoglycosides, streptothricin, trimethoprim, chloramphenicol,a putative glucose dehydrogenase precursor and a protein with unknown function. Gene cassette arrays aadB, aadA2 and dhfrA1-sat2-aadA1 were common in S.aureus isolates. We detected a completely new gene cassettes which contained aadB, oxa2, aacA4, orfD-aacA4-catB8, aadB-catB3, orfD-aacA4 and aadB-aadA1-cmlA6 of class 1 and dhfrA1-sat2-aadA1, dhfrA11, dhfrA1-sat2 of class 2 integrons.ConclusionsThis is the first study to report carriage of class 1 and 2 integrons and associated gene cassettes among in S.aureus isolates from Iran.

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Characterisation of antimicrobial resistance-associated integrons and mismatch repair gene mutations in Salmonella serotypes

Cited by 14 publications

References 25 publications

Antimicrobial Resistance and Presence of Class 1 Integrons in Salmonella Serovars Isolated from Clinical Cases of Animals and Humans in North Dakota and Uganda

Antimicrobial Resistance and Presence of Class 1 Integrons in Salmonella Serovars Isolated from Clinical Cases of Animals and Humans in North Dakota and Uganda

Mutations in gyrase and topoisomerase genes associated with fluoroquinolone resistance in Salmonella serovars from retail meats

Variability in gene cassette patterns of class 1 and 2 integrons associated with multi drug resistance patterns in Staphylococcus aureus clinical isolates in Tehran-Iran

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