Mutations in gyrase and topoisomerase genes associated with fluoroquinolone resistance in Salmonella serovars from retail meats

Yang, Baowei; Xi, Meili; Cui, Shenghui; Zhang, Xiuli; Shen, Jinling; Sheng, Min; Qu, Dong; Wang, Xin; Meng, Jianghong

doi:10.1016/j.foodres.2011.01.031

Cited by 23 publications

(11 citation statements)

References 35 publications

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“…No mutation was detected in gyrB. This observation was in agreement with previous reports that mutations in gyrA and parC were common among fluoroquinolone-resistant strains of E. coli and Salmonella, whereas mutations in gyrB were rare (Bansal et al, 2011;Yang et al, 2012).…”

Section: Discussionsupporting

confidence: 92%

Resistance patterns and detection of resistance genes in Escherichia coli isolated from diarrheic calves in Northeastern China

Jiang¹,

Zhang²

2013

Afr. J. Microbiol. Res.

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The aim of this study was to investigate the antimicrobial resistance and to characterize the implicated genes in Escherichia coli isolated from diarrheic calves during 2008-2011. A total of 104 samples were tested to 15 antimicrobial agents with disk-diffusion method. High resistance to amoxicillin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole and doxycycline was detected. A total of 13 out of the 16 resistance genes were searched in this study. The principal mechanisms of resistance found in the 104 calf isolates were blaTEM (84.6%), aadA1(73.3%), cmlA(46.2%), tet(B)(79.8%), sulI (74.0%) and dhfrV(54.8%). One kind of amino acid change in gyrA (four Ser83→Leu) and sixteen in parC (twelve 80Ser→Arg, four 80Ser→Ile) were identified in the 20 fluoroquinolone-resistant isolates. According to pulsed field gel electrophoresis (PFGE) results, clonal dissemination of multi-drug resistance (MDR) strains played an important role in the severe resistant situation among diarrheic neonatal calves in northeastern China.

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Section: Discussionsupporting

confidence: 92%

Resistance patterns and detection of resistance genes in Escherichia coli isolated from diarrheic calves in Northeastern China

Jiang¹,

Zhang²

2013

Afr. J. Microbiol. Res.

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“…Isolates that showed both NAL and CIP resistance were examined for amino acid substitutions in quinolone resistance-determination regions (QRDR) of gyrase A (GyrA) and p53-associated Parkin-like cytoplasmic protein (ParC). All genes were amplified using MyCycler PCR (Bio-Rad Laboratories Inc., Hercules, CA) as described previously (Park et al, 2006;Cattoir et al, 2007;Yang et al, 2012). The primers were synthesized by TaKaRa Biotechnology Co. Ltd. (Dalian, China) and are listed in Table 1.…”

Section: Screening For Qnr and Aminoglycoside Acetyltransferase Genesmentioning

confidence: 99%

Prevalence and characterization of Salmonella enterica in dried milk-related infant foods in Shaanxi, China

Yang

Zhao

Cui

et al. 2014

Journal of Dairy Science

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The aim of this study was to investigate the existence and characteristics of Salmonella enterica in dried milk-related infant foods. Twenty-four (3.4%) of 705 samples, including 5 (2.0%) of 246 powdered infant formula, 18 (4.0%) of 445 infant rice cereal, and 1 (7.1%) of 14 other infant foods, were positive for Salmonella. Fifteen serotypes were identified in 40 Salmonella isolates; Salmonella Duesseldorf (15.0%) and Salmonella Indiana (15.0%) were more frequently detected than other serotypes. Resistance to chloramphenicol (82.5%) was most common, followed by tetracycline (57.5%), ceftiofur (52.5%), kanamycin (52.5%), streptomycin (50.0%), gentamycin (45.0%), nalidixic acid (35.0%), ceftriaxone (32.5%), ciprofloxacin (25.0%), amikacin (20.0%), and cefoxitin (15.0%). Twenty-eight (70.0%) isolates were resistant to ≥ 8 antimicrobials, with 5 (12.5%) being resistant to 14 antimicrobials. Amino acid substitutions in gyrase A (GyrA) were most frequently detected as Ser83Arg/Asp87Glu and in p53-associated Parkin-like cytoplasmic protein (ParC), they were all Ser80Arg; the quinolone resistance gene qnrS (47.5%) was commonly detected as well as aminoglycoside acetyltransferase [aac(6')-Ib; 25.0%], qnrA (17.5%), and qnrB (15.0%) genes. Thirty distinct pulsed-field gel electrophoresis patterns were identified among 40 isolates; no identical pulsed-field gel electrophoresis pattern was detected among Salmonella isolates with the same serovar that was recovered in 2010 and 2012. Our results suggest that dried milk-related infant foods could be contaminated with Salmonella and highlight that the dangers to infant health should not be neglected.

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“…Tabela 2 -Percentual de resistência antimicrobiana de Escherichia coli isoladas de amostras de água e ostras nos estuários de Torrinhas e Taperoá, Baixo Sul, Bahia. A resistência de bactérias a amoxicilina em áreas ambientais é preocupante, uma vez que E. coli resistentes podem chegar ao homem por meio da ingestão de ostras cruas, e por conjugação conferir resistência a cepas de E. coli comensais do trato intestinal (YANG et al, 2012). Mesmo o Brasil buscando minimizar o uso abusivo de antimicrobianos ao limitar sua utilização por meio de prescrição médica, os resíduos dessas drogas excretados em fezes e urina podem chegar aos corpos hídricos, devido falhas no tratamento residual de sólidos, não detectados nas estações de tratamento (CAMPOS et al, 2013).…”

Section: Resultsunclassified

Resistência antimicrobiana de Escherichia coli em ostras de dois estuários do Baixo Sul, Bahia, Brasil

Grise

Duarte

Oliveira

et al. 2020

BJD

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Mutations in gyrase and topoisomerase genes associated with fluoroquinolone resistance in Salmonella serovars from retail meats

Cited by 23 publications

References 35 publications

Resistance patterns and detection of resistance genes in Escherichia coli isolated from diarrheic calves in Northeastern China

Resistance patterns and detection of resistance genes in Escherichia coli isolated from diarrheic calves in Northeastern China

Prevalence and characterization of Salmonella enterica in dried milk-related infant foods in Shaanxi, China

Resistência antimicrobiana de Escherichia coli em ostras de dois estuários do Baixo Sul, Bahia, Brasil

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