Characterisation of an acidic peroxidase from papaya (Carica papaya L. cv Tainung No. 2) latex and its application in the determination of micromolar hydrogen peroxide in milk

Extraction, purification and processing stability of peroxidase from plums (Prunus domestica)

“…Their ability to catalyze various redox reactions for a wide range of substrates highlights POD as being one of the most important industrial enzymes with applications in different industrial sectors, such as bioremediation, synthetic dye decolorization, polymer synthesis, biosensor development for diagnostic kit -ELISA [4] , degradation of wastewater and drinking water, and preparation of detergents. [5] Moreover, POD is one of the main quality deterioration indicator, such as flavor loss and different biodegradation reactions, being also relevant as a browning enzyme that contributes to the darkening of fruit and vegetable products during processing and storage. [6] The inactivation of POD is essential in order to minimize losses that are caused by enzymatic browning, in such a manner that several methods and technologies have been studied.…”

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

“…Membranes of 10 kDa are often utilized to concentrate both crude and purified peroxidase extract by chromatography techniques . Gottschalk et al .…”

“…Membranes of 10 kDa are often utilized to concentrate both crude 30 and purified peroxidase extract by chromatography techniques. 40,41 Gottschalk et al 56 observed that the use of 10-kDa membrane allowed the lignin peroxidase concentration for UF, which provides 90% enzymatic activity retention, 99% membrane enzyme rejection and 74% enzymatic recovery. In the current study, although the membranes tested did not show significant difference in most of the process efficiency parameters, the 10-kDa membrane was chosen due to its lower drop in permeate flux.…”

“…Ultrafiltration (UF) is commonly utilized for peroxidase concentration before or after chromatographic techniques . It consists of selective permeation of solutes in semi‐permeable, polymeric or inorganic membranes using pressure as a driving force.…”

“…35 -39 Ultrafiltration (UF) is commonly utilized for peroxidase concentration before or after chromatographic techniques. 40 -42 It consists of selective permeation of solutes in semi-permeable, polymeric or inorganic membranes using pressure as a driving force. UF can be operated in diafiltration (DF) mode, which provides better separation and high purity of the target molecule.…”