Characterisation of a novel support for biocatalysis in supercritical carbon dioxide

Immobilization and stability of a Rhizopus oryzae lipase expressed in Pichia pastoris: Comparison between native and recombinant variants

“…Usually, enzymes display an enhancement of stability after being immobilized, 28,43,44 so the effect of temperature and pH on the stability of nROL and rROL was studied in solution as well as immobilized on EP100 and Eupergit V R C. The optimum values of pH and temperature for the stability do not have to coincide with the optimum values of activity; therefore, the operation conditions selected must be a compromise between both variables. Figure 4 summarizes the experimental design.…”

Kinetics of thermal degradation and estimation of lifetime for polypropylene particles: Effects of particle size

“…Polypropylene (PP) is an interesting commodity thermoplastic polymer. Interest in PP steams from their potential applications in the areas of composites [3e5], biotechnology [6,7], powder technology [8], optoelectronics [9], catalytic support for bioreactor and waste water treatment [10,11], electrostatic enhancement application [12,13], surface and coating technology [14], etc. The use of polymer nanoparticles also includes the creation of nanostructured materials such as production of nanoscale array [15], layer blends [16], metal coated polymer particles for passive damping and energy-absorption systems [17] and flip chip packing applications [18].…”

Moving towards a Competitive Fully Enzymatic Biodiesel Process

“…Therefore, four non-commercial lipases from Pseudomonas species-LipA and LipC from P. aeruginosa 42A2 [31], LipCmut, the thermostable mutant of LipC previously described [47], and LipI.3 from Pseudomonas CR611 [21], which were produced in either homologous or heterologous hosts-were prepared, immobilized on different cheap supports, and examined for potential biodiesel production. The enzymes were obtained directly from crude cell extracts or from growth culture supernatants, and further immobilized on different carriers: Polypropylene polymers like Accurel EP100 or Accurel MP1000 [48,49] and a silica powder, Celite ® 545 [50] by simple adsorption. In this case, best immobilization was obtained on polypropylene supports: Accurel EP100 for LipA and Accurel MP1000 for LipC and LipCmut.…”