Characterisation and transferability of apple SSRs to two European pear F1 populations

Abstract

European pear (Pyrus communis L.) is among the important fruit species for which only few genetic studies have been carried out. Available evidence indicates that simple sequence repeats (SSR) are very useful as molecular markers because they are codominant, highly polymorphic, abundant and reproducible. The present paper reports more than 100 apple SSR markers in two populations of European pear; a total of 41 SSR markers were then positioned on a genetic linkage map of the cross 'Passe Crassane' x 'Harrow Sweet' and 31 in the map 'Abbe Fetel' x 'Max Red Bartlett'. Syntenic relationships between pear and apple maps have been considered for the chromosomes carrying two or more SSR markers. The alignment among the two maps supports the colinearity of the two genomes with respect both to identification and to orientation of the linkage groups.

Genetic diversity and population structure of wild pear (Pyrus pyraster (L.) Burgsd.) in Poland

“…Additionally, a large number of SSRs isolated from apple have revealed transferability to pear [11,12]. These markers have been used for Pyrus genome mapping [7,[12][13][14][15], as well as for genetic resource characterization [16][17][18][19][20][21].…”

Development and test of 21 multiplex PCRs composed of SSRs spanning most of the apple genome

“…In addition, the use of pear SSRs can be explored as it has been demonstrated that they can generally be transferred to the apple and vice versa (Yamamoto et al 2002;Pierantoni et al 2004;Silfverberg-Dilworth et al 2006) due to the high level of synteny between the two species. Nearly all apple SSRs have been mapped; the majority of them being mapped in the "reference map of the apple" (Liebhard et al 2003;Silfverberg-Dilworth et al 2006).…”

Identification of Four New Est-Based Markers on the Apple (MALUS ⋉ DOMESTICA) Genetic Map

“…Each newly developed marker increases the knowledge about the genomes and practical significance of the map. Transfer of markers became useful and reliable for comparative mapping and colinearity assessment (Pierantoni et al 2004). However, the efficiency of markers transferability is dependent on the genotype (Lewers et al 2005, Varshney et al 2005) and can be quite unsuccessful between distant genera (Gasic et al 2009).…”