Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs

Toomey, Niamh; Bolton, Declan; Fanning, Séamus

doi:10.1016/j.resmic.2009.12.010

Cited by 119 publications

(94 citation statements)

References 57 publications

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“…Contrary to our results, no Lactococcus isolates originated from traditionally fermented Indian food showed phenotypic resistance to tested antibiotics including the most representative ones among aminoglycosides, beta-lactams, cephalosporins, chloramphenicol, glycopeptides, lincosamides, macrolides and tetracyclines [53]. Toomey et al [54] reported on phenotypic resistance screen of Lactococcus lactis isolates to 6 common antibiotics (ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, and vancomycin), although molecular characterization of streptomycin resistance failed to give amplicons corresponding to any of the known streptomycin resistance genes. Our results showed a high level (62.5%) of phenotypic resistance to vancomycin among lactobacilli isolated from Sjenica cheese.…”

Section: Discussioncontrasting

confidence: 99%

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

et al. 2015

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Sjenica cheese is an artisanal cheese stored in brine, traditionally produced from raw sheep's milk in the southern part of Serbia -Sjenica Pester plateau.The aim of this study was to perform the safety evaluation of Sjenica cheese. As one of the safety criteria we considered the number of coagulase positive staphylococci and their enterotoxigenic potential. Antibiotic susceptibility/resistance patterns of autochthonous lactic acid bacteria and coagulase-positive staphylococci isolated from Sjenica cheese was also investigated.During the monitoring period of the cheese-making process, coagulase positive staphylococci did not reach the value of 10 5 cfu/g. Among coagulase positive staphylococci, 12 (46,15%) isolates showed enterotoxigenic potential and were identifi ed as Staphylococcus intermedius (11 isolates) and Staphylococcus aureus (1 isolate). Vancomycin resistance was the most prevalent phenotypic resistance profi le in coagulase positive staphylococci.Lactococci present the most dominant population among lactic acid bacteria. The most prevalent resistance phenotype in lactococci was resistance to streptomycin (83.33%), ampicillin and penicillin (70.83%); lactobacilli were characterized by resistance to vancomycin (62.5%) and tetracycline (54.17%), while resistance to streptomycin (82.46%) was the most prevalent phenotypic profi le in enterococci.All coagulase positive staphylococci and lactic acid bacteria isolates that showed resistance to tetracycline on disc diffusion and E-test, were tested for the presence of ribosomal protection proteins, tet(M) and tet(K) genes. All isolates were positive for ribosomal protection proteins genes; 14 (60.87%) isolates showed tet(M) gene presence, while 2 lactobacilli isolates revealed the presence of tet(K) gene.

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Section: Discussioncontrasting

confidence: 99%

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

et al. 2015

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“…At this concentration, P. acidilactici B14 showed a survival rate of 72 and 70.4% for 180 and 360 min, respectively. (Toomey et al 2010). The high value of the minimum inhibitory concentration (MIC> 256.0 mg/L) to vancomycin was due to the known intrinsic resistance to glycopeptides of the bacteria of this genus.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert

Ribeiro

Vandenberghe

Spier

et al. 2014

Braz. arch. biol. technol.

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“…In the past decade, attention is being given to this subject. For instance, there has been increasing evidence that points at a crucial role of foodborne LAB as reservoir of potentially transmissible AR genes and this was as a result of continuous assessment of fermented foods for presence of AR strains by several European countries (Witte, 2000;Teuber, 2001;Hammerum et al, 2007;Toomey et al, 2010). Phenotypic and molecular characterization of 121 strains of Lactobacillus paracasei isolated from Italian dairy and meat products demonstrated the presence of tetracycline [tet(M), tet(W)] and/or erythromycin resistance erm(C) in different AR isolates (Comunian et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Phenotypic and molecular characterization of 121 strains of Lactobacillus paracasei isolated from Italian dairy and meat products demonstrated the presence of tetracycline [tet(M), tet(W)] and/or erythromycin resistance erm(C) in different AR isolates (Comunian et al, 2009). Toomey et al (2010) also reported transfer of tet(M) resistance gene from L. plantarum to Lactococcus lactis BU-2-60 and E. faecalis JH2-2. The resistances of food borne LAB to glycopepetide, vancomycin and the β-lactams have been reported at different times although the reports on the latter are scanty.…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance patterns of lactic acid bacteria isolated from Nigerian grown salad vegetables

Bamidele¹,

Adeniyi²,

Fowora³

2017

Afr. J. Microbiol. Res.

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The reports on some foodborne lactic acid bacteria (LAB) possessing antibiotic resistance (AR) genes on mobile genetic elements are on the increase. In Nigeria, such information is rare. This study was therefore designed to determine the presence and locations of AR genes in LAB isolated from locally grown salad vegetables. The LAB used in this study were previously isolated from Nigerian grown cabbage, carrot, cucumber and lettuce and identified by partial sequencing of their 16S rRNA gene. The AR and integrons (intl1, 2, 3) genes were detected using polymerase chain reaction after phenotypic agar disc diffusion assay of 20 antibiotics. Extraction and curing of plasmids were performed using standard methods. Univariate analysis was performed to determine resistance to ≥ 2 antibiotics, while multinomial logistic regression was conducted to determine association of resistance patterns with vegetable sources/ types and LAB strains at 95% confidence interval (

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Characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from Irish pork and beef abattoirs

Cited by 119 publications

References 57 publications

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

Safety Evaluation Of Sjenica Cheese With Regard To Coagulase-Positive Staphylococci And Antibiotic Resistance Of Lactic Acid Bacteria And Staphylococci

Evaluation of probiotic properties of Pediococcus acidilactici B14 in association with Lactobacillus acidophilus ATCC 4356 for application in a soy based aerated symbiotic dessert

Antibiotic resistance patterns of lactic acid bacteria isolated from Nigerian grown salad vegetables

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