Antibiotic resistance patterns of lactic acid bacteria isolated from Nigerian grown salad vegetables

Abstract

The reports on some foodborne lactic acid bacteria (LAB) possessing antibiotic resistance (AR) genes on mobile genetic elements are on the increase. In Nigeria, such information is rare. This study was therefore designed to determine the presence and locations of AR genes in LAB isolated from locally grown salad vegetables. The LAB used in this study were previously isolated from Nigerian grown cabbage, carrot, cucumber and lettuce and identified by partial sequencing of their 16S rRNA gene. The AR and integrons (intl1, 2, 3) genes were detected using polymerase chain reaction after phenotypic agar disc diffusion assay of 20 antibiotics. Extraction and curing of plasmids were performed using standard methods. Univariate analysis was performed to determine resistance to ≥ 2 antibiotics, while multinomial logistic regression was conducted to determine association of resistance patterns with vegetable sources/ types and LAB strains at 95% confidence interval (

Assessment of Epiphytic Pathogens from Different Media, Associated with some Ready-for-sale Fresh-Fruits and Vegetables in Selected Markets within Southwest Nigeria

“…Bene cial microorganisms found therein could also carry the gene for pathogenicity. The prevalence of antimicrobial resistant Lactobacillus plantarum TCP 008 (cabbage) and Weissella cibaria PON 10339 (carrot), in both cabbage (32.0%) and carrot (3.0%) from Mile 12, Oshodi and others, assessed by Bamidele et al (2017) and this can bring about food-borne illness rather than the LAB to inhibit the causable agents.…”