Characterisation and fractionation studies on seed proteins of some of the leguminosae by starch‐gel electrophoresis

Adriaanse, A.; Robbers, J. E.

doi:10.1002/jsfa.2740210304

Cited by 6 publications

(2 citation statements)

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“…Isoperoxidases have been studied in peas, Pisum sativum L ., (OCKERSE et al ., 1966 ;SIEGEL & GALSTON, 1967), and protein patterns in green beans, Phaseolus vulgaris L ., and broad beans, Vicia faba L ., and peas (ADRIAANSE & ROBBERS, 1970) . ADRIAANSE & ROBBERS (1970) found that among the water-soluble seed proteins, albumin electrophoretic patterns could be used to differentiate between green and broad beans and peas, while legumin patterns showed optimum cultivar differences .…”

Section: Introductionmentioning

confidence: 99%

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Identification of broad bean cultivars based on isozyme patterns

Bassiri

Rouhani

1977

Euphytica

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Vicia faba L ., esterase, peroxidase, electrophoresis, starch gels . SUMMARYWith the increasing rate of new cultivar production for different crop plants, there is great need for methods of identifying each cultivar discriminatingly . Starch gel electrophoresis was employed to study the differences between the esterase and cathodal peroxidase isozyme patterns of 40 broad bean (Vicia faba L .) cultivars . A total of 10 and 17 medium to darkly stained bands were obtained for esterase and peroxidase systems, respectively . Bands from each enzyme system could be gropuped into three zones . Bands belonging to zone 1 of esterase (E I ) and zones 2 and 3 of peroxidase (P2 and P3) were quite distinct, stained intensely, and were especially useful for identification purposes .The differences in banding patterns among cultivars of the same origin were as great as those of cultivars of unrelated origin . A large proportion of the cultivars could be completely differentiated using both of the isozyme systems . There were no bands present in either enzyme system which were common to all cultivars .

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Section: Introductionmentioning

confidence: 99%

“…ADRIAANSE & ROBBERS (1970) found that among the water-soluble seed proteins, albumin electrophoretic patterns could be used to differentiate between green and broad beans and peas, while legumin patterns showed optimum cultivar differences . The two broad bean cultivars investigated showed many differences in the globulin fractions .…”

Section: Introductionmentioning

confidence: 99%