Chapter V Phage Typing of Klebsiella

Slopek, S

doi:10.1016/s0580-9517(08)70490-2

Cited by 8 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phage typing of Klebsiella was first developed in the 1960s (196,223). Although the phage reaction is easily read and the reproducibility of the method is acceptable, this technique shows a relatively poor typing rate of 19 to 67% (209,222). Since it is not an alternative to capsule typing, this procedure has never become widespread and is useful mainly as a secondary method in combination with serologic testing (48,53,113).…”

Section: Phage Typingmentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

View full text Add to dashboard Cite

SUMMARY Bacteria belonging to the genus Klebsiella frequently cause human nosocomial infections. In particular, the medically most important Klebsiella species, Klebsiella pneumoniae, accounts for a significant proportion of hospital-acquired urinary tract infections, pneumonia, septicemias, and soft tissue infections. The principal pathogenic reservoirs for transmission of Klebsiella are the gastrointestinal tract and the hands of hospital personnel. Because of their ability to spread rapidly in the hospital environment, these bacteria tend to cause nosocomial outbreaks. Hospital outbreaks of multidrug-resistant Klebsiella spp., especially those in neonatal wards, are often caused by new types of strains, the so-called extended-spectrum-β-lactamase (ESBL) producers. The incidence of ESBL-producing strains among clinical Klebsiella isolates has been steadily increasing over the past years. The resulting limitations on the therapeutic options demand new measures for the management of Klebsiella hospital infections. While the different typing methods are useful epidemiological tools for infection control, recent findings about Klebsiella virulence factors have provided new insights into the pathogenic strategies of these bacteria. Klebsiella pathogenicity factors such as capsules or lipopolysaccharides are presently considered to be promising candidates for vaccination efforts that may serve as immunological infection control measures.

show abstract

Section: Phage Typingmentioning

confidence: 99%

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

1998

View full text Add to dashboard Cite

show abstract

“…0-serotyping is impeded by heat stability of the capsule (14). Lysotyping is inadequate since only 60% of the strains respond and the most frequently encountered type constitutes 20% of the total (16,18). Biotyping may be useful when precise standardization of substrate concentrations is observed (2), but in our hands the API 20E was unsatisfactory since two-thirds (of 259 nonepidemic isolates) were of one biotype.…”

mentioning

confidence: 90%

Bacteriocins as tools in analysis of nosocomial Klebsiella pneumoniae infections

Bauernfeind¹,

Petermüller²,

Schneider³

1981

J Clin Microbiol

View full text Add to dashboard Cite

Epidemiological analysis of isolates from nosocomial infections caused by Klebsiella pneumoniae was improved by the use of bacteriocins in addition to capsular serotyping. Screening for bacteriocins produced by 77 reference strains for capsular serotyping identified 39 strains, and 8 of these strains were selected as a typing set. Using this set, we found that 241 to 259 (91%) nonepidemic clinical isolates of K. pneumoniae were inhibited by one or more of the eight producers. Of the most frequent bacteriocin type there were 31 examples (12%). High reproducibility of typing patterns (83.3%) and easy practicability of typing were achieved with a streak-and-point method avoiding the use of suspensions of bacteriocins and the risk of instability. The Klebsiella bacteriocins were active also on Enterobacter and Shigella species and on Escherichia coli strains, but were ineffective on other Enterobacteriacae.

show abstract

“…When several concomitant or successive cases of infection occur, typing is essential (i) to clarify if these are part of an outbreak or just coincidental, sporadic cases, and (ii) to identify the source of infection and the route of transmission. Various typing methods have been used in these epidemiological studies: K‐typing [ 7–9], biotyping [ 10,11], bacteriocin‐typing [ 12], phage typing [ 13–16], typing by pulsed field gel electrophoresis (PFGE) [ 17], ribotyping [ 18], and DNA homology analysis [ 19].…”

Section: Introductionmentioning

confidence: 99%

Twenty-three years of Klebsiella phage typing: a review of phage typing of 12 clusters of nosocomial infections, and a comparison of phage typing with K serotyping

Sechter¹,

Mestre

Hansen

2000

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

Chapter V Phage Typing of Klebsiella

Cited by 8 publications

References 13 publications

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

Klebsiellaspp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors

Bacteriocins as tools in analysis of nosocomial Klebsiella pneumoniae infections

Twenty-three years of Klebsiella phage typing: a review of phage typing of 12 clusters of nosocomial infections, and a comparison of phage typing with K serotyping

Contact Info

Product

Resources

About