Chapter IX The Measurement of Oxygen Absorption and Carbon Dioxide Evolution in Stirred Deep Cultures

Elsworth, R.

doi:10.1016/s0580-9517(08)70223-x

Cited by 5 publications

(2 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The paramagnetic [13], mass [14] and thermoconductivity [15] properties of O 2 are used to Table 1 Methods for measuring dissolved 02 (M) indicates that probes can or are always separated from the bulk liquid by an O2-permeable membrane. Errors in measurements by membrane-covered probes can arise from 02 uptake within the unstirred layer of bulk liquid adjacent to the membrane even with liquid agitation [34].…”

Section: Methods For Measuring 0 2 and Controlling Supplymentioning

confidence: 99%

How is nitrogenase regulated by oxygen?

Hill

1988

FEMS Microbiology Letters

View full text Add to dashboard Cite

Section: Methods For Measuring 0 2 and Controlling Supplymentioning

confidence: 99%

How is nitrogenase regulated by oxygen?

Hill

1988

FEMS Microbiology Letters

View full text Add to dashboard Cite

“…Gasses were mixed to the desired percentage composition by means of pre-calibrated microflowmeters. Accuracy of composition was verified by testing sayples with an Orsat Gas Analyser (Elsworth, 1970). Gas was passed from flask to flask and flow rate adjusted to 2 to 3 bubblesls by using a gas bleed on the supply line (Bartnicki-Garcia & Nickerson, 1962a).…”

Section: Introductionmentioning

confidence: 99%

The Physiology of Mould-Yeast Dimorphism in the Genus Mycotypha (Mucorales)

Hall¹,

Kolankaya²

1974

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARYMycotypha africana and four strains of Mycotypha microspora were studied. The former displayed total conversion from mycelium (M) to yeast (Y) when grown under N2 or in the presence of some electron-transport inhibitors with either glucose, fructose or mannose as carbon source. In air, mixed filamentous and yeast-like forms were frequently observed. Acid pH, high temperature, dense inoculum and high hexose level increased the proportion of the Y-form. Yeast and mycelial phases were interconvertible. All but one strains of M. microspora tested displayed similar dimorphism, but strain to strain variations were observed. I N T R O D U C T I O NMould-yeast (MY) dimorphism has been investigated in a number of pathogenic and non-pathogenic fungi from a wide range of taxonomic groups (see reviews of Romano, 1966, and Bartnicki-Garcia, I 968 a). Physiological control of this phenomenon varies between organisms ; temperature, medium composition and nature of the gas-phase have all been implicated.In the Mucorales, only the genus Mucor has been reported to display MY dimorphism (Bartnicki-Garcia & Nickerson, I 962 a). The major determining factors were the gas-phase and hexose sugar concentration (Bartnicki-Garcia, 1968 b). Yeast-like growth occurred under C 0 2 and a mycelium under N2 or air. These growth patterns were constant for Mucor rouxii over a wide range of physical and nutritional conditions but induction of the Y-phase by C 0 2 could be reversed by EDTA or other N-acetic acid type chelating agents (Bartnicki-Garcia & Nickerson, 19626). Mucor subtilissimus grew as a yeast under both N2 and C 0 2 . Hence, the existence of an EDTA-sensitive inducer of the Y-phase was postulated, the synthesis of which required C 0 2 in M. rouxii but not in M. subtilissimus, and which was thought to act on the wall polymers responsible for maintaining cell shape. A'greatly increased mannose content in the Y-phase wall (probably present as a mannose-polysaccharide) supported this suggestion (Bartnicki-Garcia & Nickerson, I 962 c).More recently Terenzi & Storck (1968) showed that 0.2 yo (v/v) of P-phenylethyl alcohol (PEA) also induced the Y-phase, inhibited the expression of cytochrome oxidase activity and stimulated alcoholic fermentation in Mucor rouxii, provided that 2% or more of a hexose was provided in the medium.In the present communication the factors controlling dimorphism in another genus of the Mucorales, namely Mycotypha, which was recently placed in the family Thamnidiaceae (Young, 1969), are described.

show abstract