Chapter IX Isolation, Cultivation and Maintenance of the Myxobacteria

Peterson, John

doi:10.1016/s0580-9517(08)70508-7

Cited by 13 publications

(9 citation statements)

References 11 publications

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“…NCIB 8501 and NCIB 9497 did not bend and had parallel sides and their cell shape resembled that in descriptions and photomicrographs of Sorangium and Chondromyces (Peterson, 1969a;Reichenbach & Dworkin, 1969), which are fruiting myxobacteria, with high % G+C (cited by Mitchell et al, 1969). Cultivated non-fruiting variants of fruiting strains are known (Dworkin, 1962;Peterson, 1969b) and NCIB 8501 and NCIB 9497, that have high % Gi-C ( Table 1 ) and are apparentlynon-fruiting (Mitchell etal., 1969), may be similar to these or to the non-fruiting strains of Stewart & Brown (1971). The last authors discussed some of the other strains of unestablished taxonomic affinity of Mitchell et al (1969, Table 5 ) but it appears that the NCIB and NCMB strains of these may be either coryneforms, or are motile, or are extremely rarely motile, Fkcv.…”

Section: Resultsmentioning

confidence: 99%

Gliding Motility in Some Non‐spreading Flexibacteria

Perry

1973

Journal of Applied Bacteriology

102

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ABSTRACT. A plate technique for detecting gliding motility is described in which glass beads placed between agar surfaces and coverslips produced surrounding areas of liquid, thus allowing cells to move freely. Some relatively short non‐spreading flexibacteria glided obviously. The movements are described and also those of myxobacteria and a fimbriate twitching bacterium. Flavobacterium aquatile F36 glided and its taxonomic status is discussed.

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Section: Resultsmentioning

confidence: 99%

Gliding Motility in Some Non‐spreading Flexibacteria

Perry

1973

Journal of Applied Bacteriology

102

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“…All plates were incubated at room temperature (20 to 22°C) and were examined periodically with a dissecting microscope for the presence of fruiting bodies. A pure culture of Angiococcus disciformis strain CMU-1 was obtained by repeated transfer of the leading edge of a vegetative swarm onto fresh media (11). Other attempts toward the isolation of A. disciformis which failed to separate the contaminants from the slime matrix involved homogenizing fruiting bodies picked from fdter paper primary enrichment plates and streaking the cell suspension onto dilute nutrient agar.…”

Section: Methodsmentioning

confidence: 99%

“…Purity of the isolate was tested by microscopic examination of the vegetative colony and by inoculating subcultures of the isolate into nutrient broth and checking for growth of contaminants after 48 h of incubation at 35°C. A. disciformis strain CMU-1 was maintained alternately on dilute nutrient agar and on a fourth medium consisting of mineral salts with a sterile rabbit dung pellet embedded in the agar (11).…”

Section: Methodsmentioning

confidence: 99%

“…The hydrolysis of casein and starch was determined by adding each substrate to dilute nutrient agar and observing for clearing around the colonies; iodine was added to the starch plate to detect the hydrolysis of starch. Cellulose hydrolysis was detected by the method of Peterson (11). Urease and deoxyribonuclease production were determined with Difco dehydrated media.…”

Section: Biochemical Tests Physiological Characteristics Ofmentioning

confidence: 99%

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Isolation, Characterization, and Emendation of Description of Angiococcus disciformis (Thaxter 1904) Jahn 1924 and Proposal of a Neotype Strain

Hook

Larkin

Brockman

1980

International Journal of Systematic Bacteriology

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Strain CMU-1, identified as a member of Angiococcus disciformis (Thaxter) Jahn 1924, was isolated from aquatic habitats in the fossa region of Davis Lake (Vestaburg Bog), Michigan. The myxobacter produced orange fruiting bodies consisting of numerous disk-shaped to oblate spheroid-shaped sporangia (average, 40 by 27 pm; 15 pm thick). The sporangia comprising the fruiting body occurred singly or were clumped in irregular masses upon a solid substrate. Mature myxospores ranged in shape from irregular spheres (1 pm in diameter) to ovals (1 by 1.5 pm). Vegetative rods were motile by gliding and measured 0.5 to 0.75 by 3 to 14 pm. The guanine plus cytosine content of the deoxyribonucleic acid extracted from A. disciformis CMU-1 was 68.4 mol%. Strain CMU-1 (ATCC 33172) has the properties recorded in the original description of Myxococcus disciformis by Thaxter, and it is herein proposed as the neotype strain of this organism. Because the myxospores were contained within sporangia, the organism cannot belong to the genus Myxococcus, and we propose that it properly belongs in the genus Angiococcus Jahn 1924.

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“…11. HAHKE 1953, 1954 REICHENBACH 1965, PETERSON 1969a, JICCURUY 1969 Braiiiisch\\-eig-Stockheinl. zur ITerfiigung gestellt wurden, herangezogen.…”

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Vorkommen und Verbreitung Fruchtkörper‐bildender Myxobakterien im Siebengebirge Vergleichende Untersuchungen unter Berücksichtigung charakteristischer Biotope

Dawid

1979

J of Basic Microbiology

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l) Gekurzte Fassung einer Dissertation der Math.-Katurw, Fakultat der Universitgt Bonn Ziel der Arheit hestand darin. die J1-sobakterienflora des Siebengebirges als eines geograp hi sc: h hegrenz t en 11 nd i i h r s c h a 1 I ha ren Gchiet es z I 1 erf amen 11 nd fest z 11 st ellen, ob und inwieweit eine Ahhiingigkit der vorhmdenen Mysohakterien-Arten und ihrcr Haiifigkeit ron Riotoptan irnd den sie hest itnuieriden tikologischen Faktorcn hesteht. Untersuchungsgehiet : Das Siebengehirge umfaDt cine t h t p p e VOII Bergen auf einer Gesamt,flache von 45 km2. Die horhsten Erhebitngen liegen h i 450 m ii. S.S., die Mehrzahl der Berge und Bergkuppen bei 2000 bis 300 in ii. S.S. Die Hauptregetation besteht. a m I~aubniisrhwald. Fagus silcrcticn. ist rorherrsrhencl. vereinzrlt sind Eiclicn-. E'ichtcii iind Kiefernbestiinde anzut,reffen. Die Gesteinslagen werderi cfurrh Rasalte. ;\ntlrxit: 'I'rwch>-t i i n t l Jlisc.ligesteine hestimmt ( F R i w I i m Knrte 1 . Unterstiohte (khiete untl Hcrkunft d~r Proben im Sichmgebirgc. ~ruchtkiirper-bildendc I\Iyxobaktcrien

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Chapter IX Isolation, Cultivation and Maintenance of the Myxobacteria

Cited by 13 publications

References 11 publications

Gliding Motility in Some Non‐spreading Flexibacteria

Gliding Motility in Some Non‐spreading Flexibacteria

Isolation, Characterization, and Emendation of Description of Angiococcus disciformis (Thaxter 1904) Jahn 1924 and Proposal of a Neotype Strain

Vorkommen und Verbreitung Fruchtkörper‐bildender Myxobakterien im Siebengebirge Vergleichende Untersuchungen unter Berücksichtigung charakteristischer Biotope

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