Chapter 5 Planar chromatography

Macek, Karel

doi:10.1016/s0301-4770(08)60866-0

Cited by 6 publications

(1 citation statement)

References 139 publications

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“…Thin-layer chromatography (TLC) is widely used for the qualitative purpose as a simple and rapid separation method for monitoring the progress of organic reactions and testing the purity of pharmaceuticals. − In most cases, chemicals separated by TLC are visually detected by fluorescence emitted from the probe reagent impregnated in the stationary phase. From the distance traveled by the analyte, the retention factor ( R f ), a useful index for analyte identification, is determined.…”

Section: Introductionmentioning

confidence: 99%

Freeze Surface-Enhanced Raman Scattering Coupled with Thin-Layer Chromatography: Pesticide Detection and Quantification Case

Fukunaga

Okada

2022

Anal. Chem.

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Thin-layer chromatography (TLC) is widely used in various branches of chemical science to separate components in complex mixtures because of its simplicity. In most cases, analyte spots are visually detected by fluorescence, and the retention factor (R f) is determined from the distance traveled by the analyte. Further characterizations are often necessary to identify separated chemicals because molecular information other than R f is not available. Surface-enhanced Raman scattering (SERS) has been coupled with TLC to complement molecular information. In previously reported TLC-SERS, metal nanoparticle suspension was dropped onto analyte spots to obtain SERS spectra. This approach is simple and efficient for SERS measurements on the TLC plate but has limited sensitivity for several reasons, such as the low solubility of analytes in the dropped solution, difficult control of nanoparticle aggregation, and interference from the stationary phase. We recently showed that freezing enhances SERS sensitivity by a factor of ∼103. Freezing simultaneously concentrates analytes and silver nanoparticles (AgNPs) in a freeze concentrated solution, where aggregation of AgNPs is facilitated, allowing sensitive freeze SERS (FSERS) measurements. Here, we discuss FSERS measurements on TLC plates to demonstrate the superiority of this combination, i.e. TLC-FSERS. Freezing enhances SERS sensitivity by freeze concentration and facilitated aggregation of AgNPs and, in addition, eliminates interference from the stationary phase. Under the optimized condition, TLC-FSERS enables the on-site detection of pesticides at the nM level. The use of the SERS signal from adenine added as the internal standard allows us to quantify pesticides. Applications to a commercial green tea beverage are also demonstrated.

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Section: Introductionmentioning

confidence: 99%

Freeze Surface-Enhanced Raman Scattering Coupled with Thin-Layer Chromatography: Pesticide Detection and Quantification Case

Fukunaga

Okada

2022

Anal. Chem.

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Thin Layer Chromatography‐Freeze Surface‐Enhanced Raman Spectroscopy: A Powerful Tool for Monitoring Synthetic Reactions

Fukunaga

Ogawa

Homma

et al. 2023

Chemistry A European J

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Thin layer chromatography (TLC) is widely used to confirm the formation of the target compound in chemical synthesis. The key issue in TLC is spot identification as it primarily relies on retention factors. Coupling of TLC with surface‐enhanced Raman spectroscopy (SERS), which gives direct molecular information, is an appropriate choice, to overcome this challenge. However, interference from the stationary phase and impurities on the nanoparticles added for SERS measurements significantly degrades TLC‐SERS efficiency. It was found that freezing effectively eliminates such interferences and dramatically improves the performance of TLC‐SERS. In this study, TLC‐freeze SERS is applied to the monitoring of four chemically important reactions. The proposed method can identify the product and side‐products of similar structures, detect compounds with high sensitivity, and provide information of quantities that allows the reliable determination of the reaction time based on kinetic analysis.

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Thin-layer and paper chromatography

Sherma¹

1988

Anal. Chem.

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Chapter 5 Planar chromatography

Cited by 6 publications

References 139 publications

Freeze Surface-Enhanced Raman Scattering Coupled with Thin-Layer Chromatography: Pesticide Detection and Quantification Case

Freeze Surface-Enhanced Raman Scattering Coupled with Thin-Layer Chromatography: Pesticide Detection and Quantification Case

Thin Layer Chromatography‐Freeze Surface‐Enhanced Raman Spectroscopy: A Powerful Tool for Monitoring Synthetic Reactions

Thin-layer and paper chromatography

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