Chapter 4 The leukotrienes and other lipoxygenase products

Effect of Dietary n-3/n-6 Fatty Acid Ratio on the Total Count, Fatty Acid Composition, and Histamine and Leukotriene Concentrations of Mast Cells in Tunica Mucosa Bronchiorum of Type I Allergic Guinea Pig

“…Mes was determined according to the method of May ef al., 16) while LT was determined by the partially modified method of Asciak et al 17) To 10 6 isolated MCs was added a sufficient amount of an L-cysteine solution to make its content 0.5% vivo The resulting mixture was pre incubated for 2 minutes, before a sufficient amount of a calcium ionophore A23187 ethanol solution was added to make its content 0.5°;() v/v. The mixture was shaken and incubated for another 10 minutes.…”

Stimulation of Prostacyclin Production in Cultured Pulmonary Artery Endothelial Cells by Pseudomonas Aeruginosa Cytotoxin: Membrane Attack and Calcium Influx

“…It fulfills many important metabolic functions and is endowed with several defense systems to ensure local blood flow and tissue integrity (for review, see Ciba Foundation Symposium, 1980). Release of prostacyclin by endothelial cells following attachment of cytotoxin may represent a line of defense in that prostacyclin counteracts several cytotoxin inflicted tissue damaging processes: in granulocytes Pseudomonas aeruginosa cytotoxin apparently stimulates the formation of leukotrienes (Bremm et al, 1983) which in turn increase the adhesiveness of other granulocytes and attract even more inflammatory cells (Hansson et al, 1983). Prostacyclin is known to lower the adhesiveness of granulocytes to the endothelium (Boxer et al, 1980) and may limit the phagocyte related vascular damage (Suttorp and Simon, 1982).…”