Stimulation of Prostacyclin Production in Cultured Pulmonary Artery Endothelial Cells by Pseudomonas Aeruginosa Cytotoxin: Membrane Attack and Calcium Influx
Abstract:The effects of highly purified Pseudomonas aeruginosa cytotoxin were investigated on cultured pulmonary artery endothelial cells. This toxin dose-dependently (7.5-60 ,ug/ml) and time-dependently (20-75 minutes) stimulated the release of radiolabeled arachidonic acid and metabolites and the synthesis of prostacyclin in the absence of overt cell damage (no enhanced lactate dehydrogenase [LDH] release). Preincubation of the toxin with neutralizing antibodies abolished the effect. The toxin response o n endothelia… Show more
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