Chapter 29. Enzyme Immunoassay

Amshey, Joseph W.

doi:10.1016/s0065-7743(08)60784-8

Cited by 2 publications

(1 citation statement)

References 52 publications

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“…These cells are being incubated in the presence of sample testes while in the control sample either the 17 β-estradiol is present (positive control) or not (negative control). Comparison of cells proliferation rates in samples tested and controls ensures possibility of determining the estrogenicity potential[1,[44][45][46].…”

mentioning

confidence: 99%

Endocrine Disrupting Compounds – Problems and Challenges

Kudłak¹,

Szczepańska²,

Owczarek³

et al. 2015

Emerging Pollutants in the Environment - Current and Further Implications

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In this chapter, information about some of the estrogenic compounds and their environmental fate and biological influence can be found. Special attention is paid to the review of the analytical approaches used at the stages of detection and determination of Endocrine Disrupting Compounds (EDCs) in the environmental samples. Also, a brief characterization of both cellular and non-cellular bioassays is presented. The discovery of micropollutants occurring in the environment resulted in new methodologies being put into the analytical practice. These methodologies are developed in two different directions. The first is based on methodological solutions designed to detect, identify, and determine xenobiotics that occur in various environmental samples. For this purpose, instrumental methods such as gas and liquid chromatography with mass spectrometry detection are usually used. The second approach is to put into the analytical practice the new bioanalytical methodologies. These methodologies allow the estimation of the sample endocrine potential, but they do not provide the information about which of the sample ingredient is responsible for causing the toxic effect. These results can be the basis for estimating the endocrine potential of the environment exhibited by certain species. Moreover, bioanalytical techniques may be supplementary to the techniques of quantitative and qualitative determination of EDCs.

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mentioning

confidence: 99%

Endocrine Disrupting Compounds – Problems and Challenges

Kudłak¹,

Szczepańska²,

Owczarek³

et al. 2015

Emerging Pollutants in the Environment - Current and Further Implications

View full text Add to dashboard Cite

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Revision of Biological Methods for Determination of EDC Presence and Their Endocrine Potential

Kudłak

Szczepańska

Owczarek

et al. 2014

Critical Reviews in Analytical Chemistry

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Endocrine-disrupting compounds (EDC) are chemicals responsible for disturbances in the hormonal balance of organisms. This group of chemicals includes both egzogenic and endogenic substances or their mixtures that impact functioning of natural hormones in organisms. In the available literature one can find information on the application of chromatographic and related techniques in the analysis of environmental samples for detection, identification, and quantitation of a wide spectrum of chemicals posing endocrine properties. On the other hand, more and more biotests are being developed to determine endocrine potency of environmental samples due to development of genetic engineering methods and specific detection methods of cells' response to the action of particular chemicals of interest. This article presents revisions of the most novel methods for this potency determination with application to biological elements.

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Chapter 29. Enzyme Immunoassay

Cited by 2 publications

References 52 publications

Endocrine Disrupting Compounds – Problems and Challenges

Endocrine Disrupting Compounds – Problems and Challenges

Revision of Biological Methods for Determination of EDC Presence and Their Endocrine Potential

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