“…Supercritical fluid chromatography (SFC) is a priori particularly attractive because 1) the viscosity of supercritical fluids (mostly carbon dioxide) is one order of magnitude smaller than that of liquid water, 2) carbon dioxide is an environment-friendly solvent, and 3) the elution strength of supercritical fluids can be easily tuned by independently adjusting its temperature, pressure, and the content of organic solvents [6][7][8] . Consequently, high-molecular-weight compounds can be easily separated at ambient/moderate temperatures (30-60 o C) by applying either high back column pressures (>2000 psi) or high contents of organic solvent up to 40% in volume 9,10 . In such conditions, the density of the mobile phase is large and the separation mechanism is similar to that encountered in LC 11,12 so that retention decreases with increasing temperature.…”