Unexpected retention and efficiency behaviors in supercritical fluid chromatography: A thermodynamic interpretation

Simultaneous determination of penthiopyrad enantiomers and its metabolite in vegetables, fruits, and cereals using ultra‐high performance liquid chromatography–tandem mass spectrometry

“…On the contrary, R-(-)-penthiopyrad has stronger interactions with the stationary phase than does S-(+)-penthiopyrad on Cellulose-4. This result clearly revealed that in the thermodynamic process, the enthalpy and entropy changes were closely related to the interactions of the solutes with the station-ary phase [30]. Setting the column temperature at 20 °C was advantageous to obtain a better resolution (Figure S5 and Figure 5, Table 3).…”

Modeling the competitive adsorption of sample solvent and solute in supercritical fluid chromatography

“…Recently, Gritti reported unexpected retention phenomena of a series of n-alkylbenzenes in SFC resulting in shifts in retention, band compression and enlargement [16]. He found that the sample solvent overloaded the C 18 column and the solvent band divided the chromatogram into three parts, each with different efficiency results.…”

High-Throughput and Ultrafast Liquid Chromatography

“…i.d.) is often used to reduce the effects of extra-column band broadening in LC, it can promote the onset of turbulent flow and increases in backpressure when used with high-flow SFC. − It should be noted, though, that recent results indicate that utilizing turbulent flow conditions may also have the potential for increasing resolution with high-throughput SFC separations of less retained compounds. − Other key factors in avoiding significant peak distortion for ultrafast SFC methods include selecting appropriate sample diluent − and organic modifiers − as well as properly maintaining column temperature control. ,− When suitable conditions are obtained, separations in the 1–10 s range are achievable for moderately complex mixtures using SFC (Figure ). …”