Chondracanthus chamissoi is an edible red seaweed with a high hydrocolloid content and food industry demand. This situation has led to a decline in their populations, especially in Peru. An alternative culture method based on the formation of secondary attachment discs (SADs) has shown several advantages over traditional spore strategies. However, there are still scarce reports of the SAD method in Peru. This work aimed to evaluate the best conditions for C. chamissoi maintenance prior to SAD development and the effect of locality on SAD formation using scallop shells as a substratum. Experiments were conducted with material collected from five localities in Pisco (Ica, Peru). Our results showed that the best conditions for C. chamissoi maintenance were: (1) fertilized seawater with Bayfolan® (0.2 mL L−1); and (2) medium exchange every two days or weekly. These conditions reduced the biomass loss to 9.36–11.14%. Most localities showed a similar capacity to produce SADs (7–17 SADs shell−1). However, vegetative algae, especially Mendieta, tended to present a higher number of SADs. Vegetative fronds also showed lower levels of necrosis and deterioration compared to cystocarpic and tetrasporophytic samples. This study shows the technical feasibility of culturing C. chamissoi through SADs for developing repopulation and/or intensive cultivation projects in Peru.