Changing patterns of vitellin-related peptides during development of the cattle tick Boophilus microplus

Canal, Cláudio Wageck; Maia, Helio; Vaz, Itabajara S.; Chies, Jocelei Maria; Farias, Nara Amélia da Rosa; Masuda, Aoi; Gonzales, João Carlos; Ozaki, Luiz Shozo; Dewes, Homero

doi:10.1007/bf00052390

Cited by 17 publications

(7 citation statements)

References 28 publications

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“…Additionally, seven Vt polypeptides were identified (Figs 7b and 8a ), and most of them increase in abundance towards the end of the feeding phase. These polypeptides are products of the five different R. microplus Vgs, as observed by tBLASTn analyses (Tables 1 and 2 ), confirming the kinetic of Vt processing observed in previous studies 27 , 33 . Accordingly, these results are consistent with the current knowledge on tick female physiology, with vitellogenesis being induced by blood meal and mediated by ecdysteroids 25 , 34 .…”

Section: Resultssupporting

confidence: 89%

“…Membranes were blocked with 5% non-fat dry milk in PBS (blocking solution), for 1 hour at room temperature. Membranes were incubated with anti-vitellin 33 or anti-VTDCE 67 rabbit sera diluted 1:100 and 1:200, respectively, in blocking solution for 16 h at 4 °C. After three washes with blocking solution, membranes were incubated with anti-rabbit antibodies conjugated with alkaline phosphatase (diluted 1:5,000 in blocking solution) for 1 hour at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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A proteomic insight into vitellogenesis during tick ovary maturation

Xavier

Tirloni

Pinto

et al. 2018

Sci Rep

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Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to categories related to basal metabolism, such as protein synthesis and modification machineries, nuclear regulation, cytoskeleton, proteasome machinery, transcriptional machinery, energetic metabolism, extracellular matrix/cell adhesion, immunity, oxidation/detoxification metabolism, signal transduction, and storage. The abundance of selected proteins involved in yolk uptake and degradation, as well as vitellin accumulation during ovary maturation, was assessed using dimethyl-labelling quantification. In conclusion, proteins identified in this study provide a framework for future studies to elucidate tick development and validate candidate targets for novel control methods.

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Section: Resultssupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

A proteomic insight into vitellogenesis during tick ovary maturation

Xavier

Tirloni

Pinto

et al. 2018

Sci Rep

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“…The major protein contained within yolk granules is vitellin (VT), a heme-containing phosphoglycolipoprotein derived from a maternal serum protein precursor, vitellogenin (Canal et al 1995). In many species, such as the tick Ornithodoros moubata (Fagotto, 1991), the cockroach Blatella germanica (Liu, McCarron & Nordin, 1996), the sea urchins Strongilocentrotus purpuratus and Lytechinus pictus (Mallya et al 1992), the frog Xenopus laevis (Fagotto & Maxfield, 1994) and the mosquito Aedes aegypti (Cho et al 1999), VT degradation is triggered by a developmentally regulated acidification of the yolk granules.…”

Section: Introductionmentioning

confidence: 99%

A Boophilus microplus vitellin-degrading cysteine endopeptidase

et al. 2003

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Here we describe the purification and characterization of a vitellin (VT) degrading cysteine endopeptidase (VTDCE) from eggs of the hard tick Boophilus microplus. A homogeneous enzyme preparation was obtained by chromatographic fractionation on ion-exchange and gel filtration columns and an autolysis step. This step consisted of incubation of a semipurified enzyme (after the first ion-exchange chromatography) at pH 4 . 0 that dissociated the enzyme from VT, to which VTDCE is naturally tightly associated. The enzyme purity was confirmed by capillary and native gel electrophoresis, and SDS-PAGE suggested the enzyme is a dimer of 17 and 22 kDa. VTDCE was active upon several synthetic substrates, with a preference for a hydrophobic or a basic residue in P 1 , and a hydrophobic residue in P 2 . VTDCE also hydrolysed haemoglobin, albumin, gelatin and vitellin. VTDCE is inactive in the absence of DTT and was totally inhibited by E-64, indicating it is a cysteine endopeptidase. Our results suggest that VTDCE is a major enzyme involved in yolk processing during B. microplus embryogenesis.

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“…As the major yolk protein, vitellin is the largest source of energy and nutrients during embryonic development and plays an irreplaceable role. Vitellin is still present in the larvae and is degraded in an orderly manner under the regulation of hydrolases [40]. The hydrolases stored in structures such as the modified lysosomes referred to as yolk granules are inactive proenzymes and they are activated mainly by developmentally controlled acidification [41].…”

Section: Discussionmentioning

confidence: 99%

Investigation of three enzymes and their roles in the embryonic development of parthenogenetic Haemaphysalis longicornis

Qiu

et al. 2020

Parasites Vectors

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Background: The tick Haemaphysalis longicornis exhibits two separate reproductive populations: bisexual and parthenogenetic, which have diploid and triploid karyotypes, respectively. The parthenogenetic population can undergo engorgement without copulation and produce viable female-only offspring with a longer incubation period than the bisexual population. Three enzymes, cathepsin B, cathepsin D and acid phosphatase, were found to be involved in vitellin degradation during the embryonic development of bisexual H. longicornis. However, the expression and activity profiles of these enzymes during the embryonic development of parthenogenetic ticks remain unknown. In the present study, the transcriptional expression profile, enzyme activity and roles in embryogenesis of the three enzymes during the embryonic development of parthenogenetic H. longicornis were investigated.Methods: Quantitative real-time polymerase chain reaction (qPCR) and fluorescence detection were used to analyze the dynamic changes in the three enzymes during embryogenesis. The roles of the three enzymes during embryogenesis were also explored using RNA interference (RNAi). Results:The three enzymes were all expressed during embryonic development in parthenogenetic H. longicornis. The expression of cathepsin B was highest on day 15, whereas that of cathepsin D was highest on day 3 and the peak of acid phosphatase expression occurred on day 9. The activity of cathepsin B was highest on day 3 and lowest on day 5, then gradually increased and remained stable. Cathepsin D activity was highest on day 1 and showed a gradually decreasing trend, whereas acid phosphatase showed the opposite trend and reached a peak on day 23. RNA interference experiments in engorged female ticks revealed that there was no significant difference in the number of eggs laid, but the hatching rate of the eggs was significantly decreased. Conclusion:The three enzymes all play important roles in embryonic development of H. longicornis, but the expression patterns and changes in the activity of the enzymes in the bisexual and parthenogenetic populations are different. The results will help a better understanding of the similarities and differences underlying embryonic development in the bisexual and parthenogenetic populations and contribute to the future exploration of the development of the parthenogenetic population of H. longicornis.

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Changing patterns of vitellin-related peptides during development of the cattle tick Boophilus microplus

Cited by 17 publications

References 28 publications

A proteomic insight into vitellogenesis during tick ovary maturation

A proteomic insight into vitellogenesis during tick ovary maturation

A Boophilus microplus vitellin-degrading cysteine endopeptidase

Investigation of three enzymes and their roles in the embryonic development of parthenogenetic Haemaphysalis longicornis

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