Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910

Huang, Tao; Tan, Hailing; Lu, Fangju; Chen, Gong; Wu, Zhenqiang

doi:10.1186/s12934-017-0828-0

Cited by 38 publications

(16 citation statements)

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“…L. plantarum ST-III was cultivated in TYC medium under air conditions and the extracellular oxido-reduction potential (ORP) was determined as described in previous studies (Zhu et al, 2014;Huang et al, 2017). In brief, after being cultured, 20 ml culture broth was filtered under vacuum through a 0.8-μm mixed cellulose ester membrane, and the filtrate was diluted appropriately to determine the ORP.…”

Section: Determination Of Extracellular Oxido-reduction Potentialmentioning

confidence: 99%

Petunidin-Based Anthocyanin Relieves Oxygen Stress in Lactobacillus plantarum ST-III

Yan¹,

Wang

Fu³

et al. 2020

Front. Microbiol.

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Application of probiotics in the food industry has been hampered by their sensitivity to challenging conditions that reduce their vitality in food matrices. A lot of attempts have been made to promote the growth of these probiotics in the aspect of nutrition demands. Among the other adverse conditions, oxygen stress can restrict the growth of probiotics and has not yet been paid enough attention to. In this study, the effect of a petunidinbased anthocyanin (ACN) on the growth of probiotic Lactobacillus plantarum ST-III was investigated under oxygen stress. The growth of ST-III was analyzed through spot assay on agar plates as well as plating-based enumeration of the viable cells in the liquid culture. Results indicated that ACN could efficiently improve the growth of ST-III under oxygen stress, whereas no effect was observed in the absence of oxygen stress. Further investigations indicated that ACN reduced the oxido-reduction potential of the culture; meanwhile, it exerted a positive transcriptional regulation on the thioredoxin system of ST-III, leading to a decrease in reactive oxygen species accumulation within the cells. Moreover, ACN enabled the growth of ST-III in reconstituted skim milk and promoted the formation of milk clots. These results revealed the role of a petunidin-based ACN in oxygen stress relief and highlighted its potential in manufacture and preservation of L. plantarumbased dairy products.

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Section: Determination Of Extracellular Oxido-reduction Potentialmentioning

confidence: 99%

Petunidin-Based Anthocyanin Relieves Oxygen Stress in Lactobacillus plantarum ST-III

Yan¹,

Wang

Fu³

et al. 2020

Front. Microbiol.

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“…Currently, it is widely used for production of yellow and red azaphilone pigments as food colorants. Recently, several research groups began conducting genetic and genomic studies of Monascus ruber (2, 3), Monascus aurantiacus (4), and Monascus purpureus (5, 6) using modern approaches to elucidate the biosynthesis of pigments and the mycotoxin citrinin. In addition, the first genome sequence of M. purpureus YY-1 was reported recently (7).…”

Section: Announcementmentioning

confidence: 99%

Whole-Genome Sequence of Monascus purpureus GB-01, an Industrial Strain for Food Colorant Production

Kumagai¹,

Tsukahara

Katayama

et al. 2019

Microbiol Resour Announc

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“…Many approaches have been applied for improvement of MonAzPs production, such as mutation breeding, process control, genetic engineering and extraction fermentation [10][11][12][13][14]. Among them, the Monascus kaoliang mutation strain produced azaphilone derivatives, such as monascusones A and B, with potential application in sunscreen cosmetics [10].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Monascus yellow pigments production by activating the cAMP signalling pathway in Monascus purpureus HJ11

Liu

et al. 2020

Preprint

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Background : Monascus azaphilone pigments (MonAzPs), which were produced by Monascus species, have been used as important food colorant and food supplements for more than one billion people during their daily life. Moreover, MonAzPs recently have received more attention because of their diverse physiological activities. However, the high microbial production of MonAzPs is still not always guaranteed. Herein, the aim of this study was to develop an efficient biotechnological process for MonAzPs production. Results: In this study, exogenous cyclic adenosine monophosphate (cAMP) treatment not only induced MonAzPs production, but also stimulated the expression of a cAMP phosphodiesterase gene, named as mrPDE , in M. purpureus HJ11. Subsequently, MrPDE was identified as a cAMP phosphodiesterase by in vitro enzymatic reaction with purified enzyme. Further, a gene knockout mutant of mrPDE was constructed to verify the activation of cAMP signalling pathway. Deletion of mrPDE in M. purpureus HJ11 improved cAMP concentration by 378 % and enhanced PKA kinase activity 1.5-fold, indicating that activation of cAMP signalling pathway was achieved. The Δ mrPDE strain produced MonAzPs at 8563 U/g, with a 2.3-fold increase compared with the WT strain. Moreover, the NAPDH/NADP + ratio of the Δ mrPDE strain was obviously higher than that of the wild type strain, which led to a higher proportion of yellow MonAzPs. With fed-batch fermentation of the Δ mrPDE strain, the production and yield of MonAzPs achieved 332.1 U/mL and 8739 U/g. Conclusions: A engineered M. purpureus strain for high MonAzPs production was successfully developed by activating the cAMP signalling pathway. This study not only describes a novel strategy for development of MonAzPs-producing strain, but also provides a roadmap for engineering efforts towards the production of secondary metabolism in other filamentous fungi.

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Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910

Cited by 38 publications

References 50 publications

Petunidin-Based Anthocyanin Relieves Oxygen Stress in Lactobacillus plantarum ST-III

Petunidin-Based Anthocyanin Relieves Oxygen Stress in Lactobacillus plantarum ST-III

Whole-Genome Sequence of Monascus purpureus GB-01, an Industrial Strain for Food Colorant Production

Enhancement of Monascus yellow pigments production by activating the cAMP signalling pathway in Monascus purpureus HJ11

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