Changing nuclear histone patterns during development

Vaughn, Jack C.; Chaitoff, J.; DeLeon, Robert Corey; Garland, C J; Thomson, Lillian A.

doi:10.1016/0014-4827(69)90215-8

Cited by 17 publications

(2 citation statements)

References 14 publications

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“…bernhardus . Because of a lack of quantitative assessment, weak staining may have led to an oversight regarding the presence of histones in decapod sperm nuclei [ 28 ]. Recent studies by Kurtz et al [ 18 , 19 ] have shown that mature spermatozoa of some crab species do have histones.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis

Kang

Guo

et al. 2015

PLoS ONE

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During spermatogenesis in most animals, the basic proteins associated with DNA are continuously changing and somatic-typed histones are partly replaced by sperm-specific histones, which are then successively replaced by transition proteins and protamines. With the replacement of sperm nuclear basic proteins, nuclei progressively undergo chromatin condensation. The Chinese Mitten Crab (Eriocheir sinensis) is also known as the hairy crab or river crab (phylum Arthropoda, subphylum Crustacea, order Decapoda, and family Grapsidae). The spermatozoa of this species are aflagellate, and each has a spherical acrosome surrounded by a cup-shaped nucleus, peculiar to brachyurans. An interesting characteristic of the E. sinensis sperm nucleus is its lack of electron-dense chromatin. However, its formation is not clear. In this study, sequences encoding histones H3 and H4 were cloned by polymerase chain reaction amplification. Western blotting indicated that H3 and H4 existed in the sperm nuclei. Immunofluorescence and ultrastructural immunocytochemistry demonstrated that histones H3 and H4 were both present in the nuclei of spermatogonia, spermatocytes, spermatids and mature spermatozoa. The nuclear labeling density of histone H4 decreased in sperm nuclei, while histone H3 labeling was not changed significantly. Quantitative real-time PCR showed that the mRNA expression levels of histones H3 and H4 were higher at mitotic and meiotic stages than in later spermiogenesis. Our study demonstrates that the mature sperm nuclei of E. sinensis contain histones H3 and H4. This is the first report that the mature sperm nucleus of E. sinensis contains histones H3 and H4. This finding extends the study of sperm histones of E. sinensis and provides some basic data for exploring how decapod crustaceans form uncondensed sperm chromatin.

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Section: Discussionmentioning

confidence: 99%

Cloning and Functional Analysis of Histones H3 and H4 in Nuclear Shaping during Spermatogenesis of the Chinese Mitten Crab, Eriocheir sinensis

Kang

Guo

et al. 2015

PLoS ONE

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“…As far as our modern knowledge goes, the embryo nic development is mainly controlled by a histonenonhistone mechanism [Vaughan, 1968;Huang and Kleinian, 1971;Desiree el al., 1973;Do and Csaba, 1973;Csaba el al., 1974;Theriault and Landesman, 1974], This control mechanism could determine which part of the genetic information -identical in each cell of the organism -becomes expressed in the different cell types. Though we do not know anything definite about the real interrelations between the regulatory proteins, on the one hand, and about those between these proteins and the DNA.…”

Section: Introductionmentioning

confidence: 99%

Appearance of free histones within nuclei of differentiating cells in the developing rat embryo

Csaba¹,

Bukta²,

Kovacs³

1977

Cells Tissues Organs

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Having applied the picric acid-bromphenol blue (BPB) and the phosphomolybdic acid-benzidine (PMA-B) methods – the first method detecting the total histone content, the second one only free histones – we have found that cell nuclei of the rat embryos permanently contain histones, nevertheless, in such a pattern that the free histone appears only at a well-defined stage of the embryogenesis. Strong PMA-B reaction is observable in the nuclei of some mesenchymal cells and in the reorganizing somites’ cell nuclei of the 13-day-old embryo as well as in the hemopoietic elements of the 15- and 17-day old embryos’ liver. Within nuclei of the red blood cells of the 13-day-old embryo an extremely intense reaction is seen which then disappears till the 15th day, while the BPB reaction shows a gradual intensification up to the neonatal age. The experiments support the former model of one of the authors for a histone-nonhistone control of ontogenesis.

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