Changing light promotes isoflavone biosynthesis in soybean pods and enhances their resistance to mildew infection

Li, Xiaoman; Yang, Caiqiong; Chen, Jianhua; He, Yuanyuan; Deng, Juzhi; Xie, Congwei; Xiao, Xinli; Long, Xiyang; Wu, Xiaoling; Liu, Weiguo; Du, Junbo; Yang, Feng; Wang, Xiaochun; Yong, Taiwen; Zhang, Jing; Wu, Yushan; Yang, Wenyu; Liu, Jiang

doi:10.1111/pce.14128

Cited by 15 publications

(9 citation statements)

References 35 publications

(56 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduction in Phe by P starvation indicates an irreversible effect of PAL genes on L-phenylalanine ammonia, as its crucial roles in plant growth and defense against various types of stresses have been reported in potato [ 73 ]. In addition, several studies have shown that light promotes catechin synthesis [ 15 ] and shade treatment is effective for the inhibition of flavonoid biosynthesis in soybean [ 29 ]. Similarly, our results support the phenomena in tea leaves whereby decreased concentrations of catechins (ECG, EGC and EGCG) were detected by half-shading under P sufficient conditions.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of Interactions between Phosphorus and Light Intensity on Metabolite Compositions in Tea Cultivar Longjing43

Long

Zhang

et al. 2022

IJMS

View full text Add to dashboard Cite

Light intensity influences energy production by increasing photosynthetic carbon, while phosphorus plays an important role in forming the complex nucleic acid structure for the regulation of protein synthesis. These two factors contribute to gene expression, metabolism, and plant growth regulation. In particular, shading is an effective agronomic practice and is widely used to improve the quality of green tea. Genotypic differences between tea cultivars have been observed as a metabolic response to phosphorus deficiency. However, little is known about how the phosphorus supply mediates the effect of shading on metabolites and how plant cultivar gene expression affects green tea quality. We elucidated the responses of the green tea cultivar Longjing43 under three light intensity levels and two levels of phosphorus supply based on a metabolomic analysis by GC×GC-TOF/MS (Two-dimensional Gas Chromatography coupled to Time-of-Flight Mass Spectrometry) and UPLC-Q-TOF/MS (Ultra-Performance Liquid Chromatography-Quadrupole-Time of Flight Mass Spectrometry), a targeted analysis by HPLC (High Performance Liquid Chromatography), and a gene expression analysis by qRT-PCR. In young shoots, the phosphorus concentration increased in line with the phosphate supply, and elevated light intensities were positively correlated with catechins, especially with epigallocatechin of Longjing43. Moreover, when the phosphorus concentration was sufficient, total amino acids in young shoots were enhanced by moderate shading which did not occur under phosphorus deprivation. By metabolomic analysis, phenylalanine, tyrosine, and tryptophan biosynthesis (PTT) were enriched due to light and phosphorus effects. Under shaded conditions, SPX2 (Pi transport, stress, sensing, and signaling), SWEET3 (bidirectional sugar transporter), AAP (amino acid permeases), and GSTb (glutathione S-transferase b) shared the same analogous correlations with primary and secondary metabolite pathways. Taken together, phosphorus status is a crucial factor when shading is applied to increase green tea quality.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Furthermore, amino acids are responsible for tea quality. For instance, low-quality grades of black tea contain lower free amino acid contents [ 29 ]. The most abundant amino acids in tea are theanine (Thea), glutamine (Gln), glutamic acid (Glu), and arginine (Arg) [ 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Interactions between Phosphorus and Light Intensity on Metabolite Compositions in Tea Cultivar Longjing43

Long

Zhang

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Metabolic pathways are significantly different in soybean inoculated with Fusarium Verticillium compared to normal soybean. Flavonoid contents are significantly higher in soybean inoculated with molds, and Ja induces the synthesis of biomacromolecules such as glycine to enhance soybean resistance [ 56 ]. Moreover, mannitol, threitol and trehalose were significantly enriched in Armillaria luteobuablina -treated roots [ 57 ].…”

Section: Application Of Metabolomics In the Field Of Fungal Researchmentioning

confidence: 99%

Application of Metabolomics in Fungal Research

Jian

Liu

et al. 2022

Molecules

View full text Add to dashboard Cite

Metabolomics is an essential method to study the dynamic changes of metabolic networks and products using modern analytical techniques, as well as reveal the life phenomena and their inherent laws. Currently, more and more attention has been paid to the development of metabolic histochemistry in the fungus field. This paper reviews the application of metabolomics in fungal research from five aspects: identification, response to stress, metabolite discovery, metabolism engineering, and fungal interactions with plants.

show abstract

“…As explained by the biodiversity theory, the maize-soybean intercropping system is an ecological strategy to control or relieve diseases ( Zhang et al., 2019 ). In intercropping system, soybean undergoes complex changes in light environment ( Li et al., 2021 ). During the symbiotic period of soybean and corn, soybean was in the shade.…”

Section: Introductionmentioning

confidence: 99%

Soybean balanced the growth and defense in response to SMV infection under different light intensities

et al. 2023

View full text Add to dashboard Cite

Light is essential for the growth and defense of soybean. It is not clear how soybeans adjust their defenses to different light environments with different cropping patterns. The mechanism of soybean response to Soybean mosaic virus (SMV) infection under different light intensities was analyzed by RNA-seq sequencing method. Enrichment analysis illustrated that most defense-related genes were down-regulated in the dark and the shade, and up-regulated under hard light and normal light. Soybean can resist SMV infection mainly by activating salicylic acid signaling pathway. Light is essential for activating salicylic acid defense signaling pathways. With the increase of light intensity, the oxidative damage of soybean leaves was aggravated, which promoted the infection of virus. When light was insufficient, the growth of soybean was weak, and the plant-pathogen interaction pathway, MAPK pathway and hormone defense pathway in infected soybean was inhibited. Under hard light, some defense genes in infected soybean were down-regulated to reduce the degree of oxidative damage. The expression of differentially expressed genes was verified by real-time fluorescence quantitative RT-PCR. In order to adapt to the change of light intensity, soybean balanced allocation of resources between growth and defense through a series regulation of gene expression. The results of this study will provide a theoretical basis for the research of SMV resistance in intercropping soybean.

show abstract

Changing light promotes isoflavone biosynthesis in soybean pods and enhances their resistance to mildew infection

Cited by 15 publications

References 35 publications

Effect of Interactions between Phosphorus and Light Intensity on Metabolite Compositions in Tea Cultivar Longjing43

Effect of Interactions between Phosphorus and Light Intensity on Metabolite Compositions in Tea Cultivar Longjing43

Application of Metabolomics in Fungal Research

Soybean balanced the growth and defense in response to SMV infection under different light intensities

Contact Info

Product

Resources

About