Application of Metabolomics in Fungal Research

Li, Guangyao; Jian, Tongtong; Liu, Xiaojin; Lv, Qingqing; Zhang, Guoying; Ling, Jianya

doi:10.3390/molecules27217365

Cited by 20 publications

(25 citation statements)

References 139 publications

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“…Metabolomics measure a group metabolites from organisms and has been a staple tool for systems biology for decades (Kell, 2004). Application of metabolomics in fungal research, including the subfield of marine fungi, has largely focused on the measurements of secondary metabolites and usually driven by the interests in natural product discovery (G. Li et al., 2022; Stuart et al., 2020). Metabolic profiling of marine fungi captures snapshots of fungal metabolites under different growth conditions.…”

Section: Methods For Studying Planktonic Marine Fungimentioning

confidence: 99%

“…Metabolic profiling of marine fungi captures snapshots of fungal metabolites under different growth conditions. Dissolved metabolites in the aqueous phase are the most common type of metabolites analyzed using moder instruments such as high‐performance liquid chromatography‐mass spectrometry (G. Li et al., 2022). Very few metabolomic studies have been performed using planktonic marine fungi as subjects.…”

Section: Methods For Studying Planktonic Marine Fungimentioning

confidence: 99%

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Planktonic Marine Fungi: A Review

Peng,

Amend,

Baltar

et al. 2024

JGR Biogeosciences

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Fungi in marine ecosystems play crucial roles as saprotrophs, parasites, and pathogens. The definition of marine fungi has evolved over the past century. Currently, “marine fungi” are defined as any fungi recovered repeatedly from marine habitats that are able to grow and/or sporulate in marine environments, form symbiotic relationships with other marine organisms, adapt and evolve at the genetic level, or are active metabolically in marine environments. While there are a number of recent reviews synthesizing our knowledge derived from over a century of research on marine fungi, this review article focuses on the state of knowledge on planktonic marine fungi from the coastal and open ocean, defined as fungi that are in suspension or attached to particles, substrates or in association with hosts in the pelagic zone of the ocean, and their roles in remineralization of organic matter and major biogeochemical cycles. This review differs from previous ones by focusing on biogeochemical impacts of planktonic marine fungi and methodological considerations for investigating their diversity and ecological functions. Importantly, we point out gaps in our knowledge and the potential methodological biases that might have contributed to these gaps. Finally, we highlight recommendations that will facilitate future studies of marine fungi. This article first provides a brief overview of the diversity of planktonic marine fungi, followed by a discussion of the biogeochemical impacts of planktonic marine fungi, and a wide range of methods that can be used to study marine fungi.

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Section: Methods For Studying Planktonic Marine Fungimentioning

confidence: 99%

Section: Methods For Studying Planktonic Marine Fungimentioning

confidence: 99%

Planktonic Marine Fungi: A Review

Peng,

Amend,

Baltar

et al. 2024

JGR Biogeosciences

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“…Various fungal metabolites become important targets of antifungal exposure [104]. The use of the created biomimetics is aimed at their competition with the substituted molecules, which affects the metabolic activity and growth of fungal cells [105]. The well-known metabolomics of fungi enable developing of approaches to the decrease in fungal resistance and adaptation to various stress conditions (ionizing radiation, pH change, hypoxic stress, solar ultraviolet radiation, presence of toxic compounds, nutrient stress, oxidative stress, cold-heat stress) (Table 5) [17,89,[106][107][108][109][110][111][112][113].…”

Section: Biomimetics Of Metabolites With Effect On Growth and Metabol...mentioning

confidence: 99%

Various Biomimetics as Antifungals

Efremenko,

Aslanli,

Stepanov

et al. 2023

Preprint

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Biomimetics, which are similar to natural compounds that play an important role in the metabolism, manifestation of functional activity and reproduction of various fungi, have a pronounced attrac-tion in the current search for new effective antifungals. Actual trends in the development of this area of research indicate that unnatural amino acids can be used as such biomimetics, including those containing halogen atoms; compounds similar to nitrogenous bases embedded in the nucleic acids synthesized by fungi; peptides imitating fungal analogues; molecules similar to natural sub-strates of numerous fungal enzymes and Quorum Sensing signaling molecules of fungi and yeast; etc. Most part of the review is devoted to the analysis of semi-synthetic and synthetic antifungal peptides and their targets of action. This review is aimed at combining and systematizing the cur-rent scientific information accumulating in this area of research, developing various antifungals with an assessment of the effectiveness of the created biomimetics and the possibility of combining them with other antimicrobial substances to reduce cell resistance and improve antifungal effects.

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“…Recently, it has been increasingly used to identify microbial metabolites, which are promising sources of bioactive compounds with potential applications in medicine, agriculture, and industry 14 – 17 . Its application in fungal research has helped in five aspects: identification, response to stress, metabolite discovery, metabolism engineering, and fungal interactions with plants 18 . Untargeted metabolomics and bioinformatics were applied to set up biomarkers of aflatoxigenic Aspergillus species 19 .…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling and in-silico studies show multiple effects of insecticidal actinobacterium on Spodoptera littoralis

Diab,

Mead,

Khedr

et al. 2024

Sci Rep

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The polyphagous pest, Spodoptera littoralis (Boisduval), poses a significant global economic threat by gregariously feeding on over a hundred plant species, causing substantial agricultural losses. Addressing this challenge requires ongoing research to identify environmentally safe control agents. This study aimed to elucidate the insecticidal activity of the metabolite (ES2) from a promising endophytic actinobacterium strain, Streptomyces sp. ES2 EMCC2291. We assessed the activity of ES2 against the eggs and fourth-instar larvae of S. littoralis through spectrophotometric measurements of total soluble protein, α- and β-esterases, polyphenol oxidase (PPO), and catalase enzyme (CAT). The assessments were compared to commercial Biosad® 22.8% SC. Untargeted metabolomics using LC-QTOF-MS/MS identified 83 metabolic compounds as chemical constituents of ES2. The median lethal concentration (LC50) of ES2 (165 mg/mL) for treated Spodoptera littoralis eggs showed significant differences in polyphenol oxidase and catalase enzymatic activities, while the LC50 of ES2 (695 mg/mL) for treated S. littoralis fourth instar larvae showed lower significance in α- and β-esterase activities. Molecular docking of ES2 identified seven potent biocidal compounds, showing strong affinity to PPO and catalase CAT proteins in S. littoralis eggs while displaying limited binding to alpha and beta esterase proteins in the larvae. The results contribute to the understanding of ES2 as a promising alternative biopesticide, providing insights for future research and innovative applications in sustainable pest management strategies.

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Application of Metabolomics in Fungal Research

Cited by 20 publications

References 139 publications

Planktonic Marine Fungi: A Review

Planktonic Marine Fungi: A Review

Various Biomimetics as Antifungals

Metabolite profiling and in-silico studies show multiple effects of insecticidal actinobacterium on Spodoptera littoralis

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