Changing expression profiles of lncRNAs, mRNAs, circRNAs and miRNAs during osteoclastogenesis

Abstract

Bone is a dynamic organ continuously undergoing shaping, repairing and remodeling. The homeostasis of bone is maintained by the balance between osteoblastic bone formation and osteoclastic bone resorption. Osteoclasts (OCs) are specialized multinucleated cells derived from hematopoietic stem cells (HSCs) or monocytes/macrophage progenitor cells. There are different stages during osteoclastogenesis, and one of the most important steps to form functional osteoclasts is realized by cell-cell fusion. In our study, microarray was performed to detect the expression profiles of lncRNA, mRNA, circRNA and miRNA at different stages during osteoclastogenesis of RAW264.7 cells. Often changed RNAs were selected and clustered among the four groups with Venn analysis. The results revealed that expressions of 518 lncRNAs, 207 mRNAs, 24 circRNAs and 37 miRNAs were often altered at each stage during OC differentiation. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analysis were performed to predict the functions of differentially expressed lncRNAs and co-expressed potential targeting genes. Co-expression networks of lncRNA-mRNA and circRNA-miRNA were constructed based on the correlation analysis between the differentially expressed RNAs. The present study provided a systematic perspective on the potential function of non-coding RNAs (ncRNAs) during osteoclastogenesis.

The Circular RNA Landscape of Periodontal Ligament Stem Cells During Osteogenesis

“…The dynamic expression changed during osteoblast differentiation and biomineralization of PDLSCs in a temporal manner, suggesting that circRNAs may play a role in osteoblast differentiation and bone formation. Moreover, induction of osteoclast differentiation has recently been associated with change of circRNA profiles 32 . Considering the relatively stable structure, circRNAs can be used as biomarkers for diagnosis and prognosis of bone diseases.…”

LncRNA-AK131850 Sponges MiR-93-5p in Newborn and Mature Osteoclasts to Enhance the Secretion of Vascular Endothelial Growth Factor a Promoting Vasculogenesis of Endothelial Progenitor Cells

“…The procedure of inducing osteoclast different stages during osteoclastogenesis was admitted and described in previous study [16, 39, 40]. In brief, RAW264.7 cells before 3 passages were cultured with osteoclast differentiation medium consisting of DMEM supplemented with 10% FBS, 1% penicillin-streptomycin, 100 ng/ml receptor activator of nuclear factor κB ligand (RANKL; R&D Systems, Minneapolis, MN, USA) and 50 ng/ml macrophage colony stimulating factor (M-CSF; R&D Systems, Minneapolis, MN, USA) for 24 h, 72 h and 96 h to gain Pre-OC, N-OC and M-OC, respectively.…”

“…OCs are specialized multinucleated cells derived from hematopoietic stem cells or monocytes/macrophage progenitor cells [15]. There are three different stages during osteoclastogenesis: preosteoclast (Pre-OC), newborn osteoclast (N-OC) and mature osteoclast (M-OC) [16-18]. EPCs are a population of cells that are found circulating in the bone marrow and peripheral blood, which have the ability to differentiate into mature EC and then participate in vasculogenesis.…”

Comprehensive circRNA expression profile and construction of circRNA‐associated ceRNA network in fur skin

“…Thus, understanding the characteristics and functions of melanocyte at the molecular and genetic levels may provide crucial information for the development of new regenerative therapies for melanoma. Recently, studies have shown that circular RNAs (circRNAs) have vital roles in a variety of metabolism pathways . Nevertheless, little is known about the functions of circRNAs in the pigmentation pathways, and the role of circRNAs in the melanocyte remains unknown.…”