Changes of the plastid system of Euglena gracilis induced with streptomycin and dihydrostreptomycin

Ebringer, L.; Němec, Pavel; Santová, H; Foltínová, P

doi:10.1007/bf00410628

Cited by 22 publications

(9 citation statements)

References 23 publications

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“…The first marked difference in biological action of streptomycin and dihydrostreptomycin was also observed with the help ofE. gracilis (9,10). We similarly found differences in biological activities of various mitomycins (8).…”

supporting

confidence: 69%

“…Such a permanent loss of plastids is referred to as "hereditary bleaching." It was proved that all so-called "bleaching antibiotics" affect chloroplasts, which become pathological and are gradually diluted out of dividing cells (3,4,10). A simultaneous antibacterial and antiplastid activity of many antibiotics was observed.…”

mentioning

confidence: 99%

“…Methods of cultivation, determination of chlorophyll, counting irreversibly bleached organisms, and studying the chloroplasts were as published in a previous paper (10).…”

mentioning

confidence: 99%

“…A final concentration of 1,000 ,ug of 5-NFA per ml was used, and the maximal time of the treatment was 60 min. The reaction mixture was incubated in a water bath at 37 C, and samples were taken at selected time intervals (10,14,20,32,45, and 60 min) and pipetted into a 10-fold volume of a 4 C cold buffer and immediately centrifuged. After the centrifugation the suspension was adjusted to the original volume with physiological saline containing Tween 80, diluted, and transferred to a solid medium with tryptose (15) and to Davis minimal medium to determine the viable cell count and the number of reversions, respectively.…”

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confidence: 99%

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Mutagenic Action of Nitrofurans on Euglena gracilis and Mycobacterium phlei

Ebringer

Jurasek²,

Koníčék³

et al. 1976

Antimicrob Agents Chemother

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There is a pronounced difference between the action of antibiotics and nitrofurans on Euglena gracilis . Those antibiotics that induce hereditary loss of chloroplasts do so only when they affect dividing cells. On the other hand, nitrofurans induce a mass mutation in both dividing and nondividing cells (under conditions of continuous illumination of cultures). It was found that a breakdown product, 5-nitro-2-furaldehyde, is liberated from furadantin and furoxone. This intermediate is responsible for the observed specific mutagenicity of 5-nitrofuran drugs. The mutagenic action of 5-nitro-2-furaldehyde is very similar to that of nitrosoguanidine. Both compounds induce bleached mutants of E. gracilis when acting on growing or resting cells, regardless of the dark or light conditions. Similarly, both compounds induce reverse mutations in auxotrophic strains of Mycobacterium phlei .

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supporting

confidence: 69%

mentioning

confidence: 99%

“…Methods of cultivation, determination of chlorophyll, counting irreversibly bleached organisms, and studying the chloroplasts were as published in a previous paper (10).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Mutagenic Action of Nitrofurans on Euglena gracilis and Mycobacterium phlei

Ebringer

Jurasek²,

Koníčék³

et al. 1976

Antimicrob Agents Chemother

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“…The inhibition of chloroplast development (S. Schwartzbach and J. A. Schiff, manuscript in preparation) and replication (18,19) produced by SM could be reversed if the cells were treated with hydroxylamine (HA), but HA treatment did not reverse the action of DSM (18,19). The reaction product of SM and HA, SM oxime, is biologically inactive (4, 7) in bacterial systems.…”

mentioning

confidence: 99%

Chloroplast and Cytoplasmic Ribosomes of Euglena : Selective Binding of Dihydrostreptomycin to Chloroplast Ribosomes

Schwartzbach

Schiff

1974

J Bacteriol

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Dihydrostreptomycin binds preferentially to chloroplast ribosomes of wild-type Euglena gracilis Klebs var. bacillaris Pringsheim. The K diss for the wild-type chloroplast ribosome-dihydrostreptomycin complex is 2 × 10 −7 M, a value comparable with that found for the Escherichia coli ribosome-dihydrostreptomycin complex. Chloroplast ribosomes isolated from the streptomycin-resistant mutant Sm 1 r BNgL and cytoplasmic ribosomes from wild-type have a much lower affinity for the antibiotic. The K diss for the chloroplast ribosome-dihydrostreptomycin complex of Sm 1 r is 387 × 10 −7 M, and the value for the cytoplasmic ribosome-dihydrostreptomycin complex of the wild type is 1,400 × 10 −7 M. Streptomycin competes with dihydrostreptomycin for the chloroplast ribosome binding site, and preincubation of streptomycin with hydroxylamine prevents the binding of streptomycin to the chloroplast ribosome. These results indicate that the inhibition of chloroplast development and replication in Euglena by streptomycin and dihydrostreptomycin is related to the specific inhibition of protein synthesis on the chloroplast ribosomes of Euglena .

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Der Atmungsdefekt bei Hefezellen: eine kritische Betrachtung seiner Ursachen II. Der „RD‐Zustand”︁ (petite‐Mutation) und verwandte Defekte

Kraepelin

1972

J of Basic Microbiology

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EinleitungI n der Zeit seit dem Erscheinen des ersten Teiles dieses Sammelreferates (KRAEPELIN 1967) hat der Atmungsdefekt, soweit er sich auf die sogenannte plasmatische ,,petite-Mutation" bezieht, noch keine definitive Kliirung gefunden. Obgleich es zu diesem Thema bereits mehrere zusammenfassende Darstellungen gibt, in denen die zur Zeit wesentlich erscheinenden Fakten diskutiert wurden, mag die in manchem abweichende Betrachtungsweise dieses Referat dennoch rechtfertigen. Insbesondere mag es dazu beitragen, bekannte aber scheinbar unkorrelierte Phiinomene einmal im Zusammenhang zu sehen. Drt es noch keineswegs erwiesen ist, daD der plasmatische Atmungsdefekt und seine Begleiterscheinungen auf einer Mutation genetischen Materials beruhen, sol1 hier der kausal weniger festgelegten Bezeichnung ,,RD-Zustand" (respiratory defective state) auch weiterhin der Vorzug gegeben werdena). 17'gen. n., the heterobasidiomycetous stage of several yeasts of the genus Candida. Antonie van Leeuwenhoek, 86,433-462. FIIL, A. and BRANTON, D., 1969. Changes in the plasma membrane of Escherichia coli during magnesium starvation.

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Changes of the plastid system of Euglena gracilis induced with streptomycin and dihydrostreptomycin

Cited by 22 publications

References 23 publications

Mutagenic Action of Nitrofurans on Euglena gracilis and Mycobacterium phlei

Mutagenic Action of Nitrofurans on Euglena gracilis and Mycobacterium phlei

Chloroplast and Cytoplasmic Ribosomes of Euglena : Selective Binding of Dihydrostreptomycin to Chloroplast Ribosomes

Der Atmungsdefekt bei Hefezellen: eine kritische Betrachtung seiner Ursachen II. Der „RD‐Zustand”︁ (petite‐Mutation) und verwandte Defekte

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