Changes of FAME profiles as a marker of phenol degradation in different soils inoculated with Pseudomonas sp. CF600

Mrozik, Agnieszka; Cycoń, Mariusz; Piotrowska‐Seget, Zofia

doi:10.1016/j.ibiod.2009.11.002

Cited by 34 publications

(26 citation statements)

References 49 publications

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“…It should be said that B. xenovorans LB400 strain produced smaller amount of these fatty acids when cultivated with addition of natural terpenes than with PCBs alone. Our conclusions are supported by results of other researches investigating the role of these fatty acids in protection of bacterial strains against adverse stress effects of toxic aromatic compounds (Mrozik et al 2005(Mrozik et al , 2010Shabala and Ross 2008).…”

Section: Discussionsupporting

confidence: 88%

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Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

Zorádová-Murínová

Dudášová

Lukáčová

et al. 2011

Appl Microbiol Biotechnol

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In this study, we examined the effect of polychlorinated biphenyls (PCBs) in the presence of natural and synthetic terpenes and biphenyl on biomass production, lipid accumulation, and membrane adaptation mechanisms of two PCB-degrading bacterial strains Pseudomonas stutzeri and Burkholderia xenovorans LB400. According to the results obtained, it could be concluded that natural terpenes, mainly those contained in ivy leaves and pine needles, decreased adaptation responses induced by PCBs in these strains. The adaptation processes under investigation included growth inhibition, lipid accumulation, composition of fatty acids, cis/trans isomerization, and membrane saturation. Growth inhibition effect decreased upon addition of these natural compounds to the medium. The amount of unsaturated fatty acids that can lead to elevated membrane fluidity increased in both strains after the addition of the two natural terpene sources. The cells adaptation changes were more prominent in the presence of carvone, limonene, and biphenyl than in the presence of natural terpenes, as indicated by growth inhibition, lipid accumulation, and cis/trans isomerization. Addition of biphenyl and carvone simultaneously with PCBs increased the trans/cis ratio of fatty acids in membrane fractions probably as a result of fluidizing effects of PCBs. This stimulation is more pronounced in the presence of PCBs as a sole carbon source. This suggests that PCBs alone have a stronger effect on bacterial membrane adaptation mechanisms than when added together with biphenyl or natural or synthetic terpenes.

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Section: Discussionsupporting

confidence: 88%

“…This phenomenon is thought to be the major adaptive mechanism in growing microorganisms exposed to toxic aromatic compounds. Due to it, the bacterial membranes become more resistant to the fluidizing action of aromatic compounds, which allows the cells to survive in hydrocarboncontaminated sites (Mrozik et al 2005(Mrozik et al , 2010.…”

Section: Introductionmentioning

confidence: 99%

Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

Zorádová-Murínová

Dudášová

Lukáčová

et al. 2011

Appl Microbiol Biotechnol

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show abstract

“…Disappearance of β-CP and 3-PBA in soil was fitted to first-order kinetic model (Cycoń et al 2009(Cycoń et al , 2011Mrozik et al 2010). The rate constant (k) was determined using the algorithm as expressed in Eq.…”

Section: Discussionmentioning

confidence: 99%

Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01

Chen

Geng

Xiao

et al. 2011

Appl Microbiol Biotechnol

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Using laboratory and field experiments, the ability of Streptomyces aureus HP-S-01 to eliminate β-cypermethrin (β-CP) and its metabolite 3-phenoxybenzaldehyde (3-PBA) in soils was investigated. In the laboratory, 80.5% and 73.1% of the initial dose of β-CP and 3-PBA (50 mg kg(-1)) was removed in sterilized soils within 10 days, respectively, while in the same period, disappearance rate of β-CP and 3-PBA in non-sterilized soils was higher and reached 87.8% and 79.3%, respectively. Furthermore, the disappearance process followed the first-order kinetics and the half-life (T (1/2)) for β-CP and 3-PBA reduced by 20.3-52.9 and 133.7-186.8 days, respectively, as compared to the controls. The addition of sucrose to the soils enhanced the ability of strain HP-S-01 to eliminate β-CP and 3-PBA. Similar results were observed in the field experiments. The introduced strain HP-S-01 quickly adapted to the environment and rapidly removed β-CP and 3-PBA without any lag phases in the field experiments. Compared with the controls, 47.9% and 67.0% of applied dose of β-CP and 3-PBA was removed from the soils without extra carbon sources and 52.5% and 73.3% of β-CP and 3-PBA was eliminated in soils supplemented with sucrose within 10 days, respectively. Analysis of β-CP degradation products in soil indicated that the tested strain transform β-CP to 3-PBA and α-hydroxy-3-phenoxy-benzeneacetonitrile. However, both intermediates were transient and they disappeared after 10 days. Therefore, the selected actinomyces strain HP-S-01 is suitable for the efficient and rapid bioremediation of β-CP contaminated soils.

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“…These results were observed under the exposure of polycyclic aromatic hydrocarbons (PAHs), phenols, biphenyl, and polychlorinated biphenyls (PCBs) [7, 28, 61, 62]. …”

Section: Adaptation Mechanisms Of Bacterial Strainsmentioning

confidence: 99%

Response Mechanisms of Bacterial Degraders to Environmental Contaminants on the Level of Cell Walls and Cytoplasmic Membrane

Murínová

Dercová

2014

International Journal of Microbiology

151

106

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Bacterial strains living in the environment must cope with the toxic compounds originating from humans production. Surface bacterial structures, cell wall and cytoplasmic membrane, surround each bacterial cell and create selective barriers between the cell interior and the outside world. They are a first site of contact between the cell and toxic compounds. Organic pollutants are able to penetrate into cytoplasmic membrane and affect membrane physiological functions. Bacteria had to evolve adaptation mechanisms to counteract the damage originated from toxic contaminants and to prevent their accumulation in cell. This review deals with various adaptation mechanisms of bacterial cell concerning primarily the changes in cytoplasmic membrane and cell wall. Cell adaptation maintains the membrane fluidity status and ratio between bilayer/nonbilayer phospholipids as well as the efflux of toxic compounds, protein repair mechanisms, and degradation of contaminants. Low energy consumption of cell adaptation is required to provide other physiological functions. Bacteria able to survive in toxic environment could help us to clean contaminated areas when they are used in bioremediation technologies.

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Changes of FAME profiles as a marker of phenol degradation in different soils inoculated with Pseudomonas sp. CF600

Cited by 34 publications

References 49 publications

Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

Adaptation mechanisms of bacteria during the degradation of polychlorinated biphenyls in the presence of natural and synthetic terpenes as potential degradation inducers

Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01

Response Mechanisms of Bacterial Degraders to Environmental Contaminants on the Level of Cell Walls and Cytoplasmic Membrane

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