Changes of enzymatic activity during a large-scale vermicomposting process with continuous feeding

Hřebečková, Tereza; Wiesnerová, Lucie; Hanč, Aleš

doi:10.1016/j.jclepro.2019.118127

Cited by 34 publications

(6 citation statements)

References 31 publications

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“…The activity of β- d -glucosidase in the test pile decreased with the age of the vermicompost (from 1225.2 µmol MUFG h −1 g −1 to 611.4 µmol MUFG h −1 g −1 ), but the trend was the opposite for the control pile (increased from 1253.0 µmol MUFG h −1 g −1 to 2498.4 µmol MUFG h −1 g −1 ). The activity of β- d -glucosidase measured by Hřebečková et al (2019b) in the vermicomposting heaps with household biowaste, malting sludge and grape marc was lower than the activity measured in the control pile. The highest value of β- d -glucosidase in their experiment was 1642 µmol MUFG h −1 g −1 in the vermicomposting heap with grape marc.…”

Section: Resultsmentioning

confidence: 60%

“…Groups of microorganisms were detected using analyses of free methyl esters of PLFA, according to Hanč et al (2017). The enzymatic activity of hydrolytic enzymes (β- d -glucosidase, acid phosphatase, arylsulphatase, lipase, chitinase, cellobiohydrolase, alanine aminopeptidase, leucine aminopeptidase) were measured as the change in absorbance in 96-well microplates using Tecan Infinite® M200 (Tecan, Grödig, Austria), according to Hřebečková et al (2019b).…”

Section: Methodsmentioning

confidence: 99%

“…The dry matter was determined gravimetrically as the difference between before and after drying in a dryer at 35 °C according to Hřebečková et al (2019b). The value of active pH and electrical conductivity (EC) was determined for fresh samples with demineralized water 1:5 according to the standard BSI EN 15933 (2012).…”

Section: Agrochemical Analysesmentioning

confidence: 99%

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Problems associated with vermicomposting of dog excrement in practice using Eisenia andrei

et al. 2022

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One 25-kg dog produces about 500 g of excrement per day. Excrement is a potentially hazardous material, as it may contain pathogenic microorganisms. Our samples were tested for the presence of thermotolerant coliform bacteria, Enterococcus spp., Escherichia coli and Salmonella spp., which are indicators of faecal contamination, as well as for the presence of helminths and their eggs. During the experiment, it was observed whether these microorganisms could be eliminated by vermicomposting. There were two variants of vermicomposting piles: one test pile (with continuous feeding) and one control pile (with a single feeding). The vermicomposting process was run in outdoor conditions in park for 51 weeks using Eisenia andrei earthworms. The vermicomposting of dog excrement with waste from park maintenance (1:2) can produce a good quality fertiliser. During the process of vermicomposting, there was a gradual decrease in the content of pathogenic bacteria. At the end of the vermicomposting process, there were no eggs or adult helminths. The vermicompost was very rich in microorganisms and enzymatic activity. The pH value was slightly alkaline, and the C:N ratio corresponded to value of mature vermicompost.

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Section: Resultsmentioning

confidence: 60%

Section: Methodsmentioning

confidence: 99%

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Problems associated with vermicomposting of dog excrement in practice using Eisenia andrei

et al. 2022

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“…Less microbial biomass, mainly fungi, was found in the earthworm treatments, which is consistent with the claim that fungi are a valuable food source ( Zhang et al, 2000 ). Hřebečková et al (2019) compared the enzymatic activity in three aged vermicompost types (from household biowaste, malt house sludge mixed with agricultural waste, and grape marc). The vermicomposting was conducted in large-scale heap systems with continuous earthworm feeding, which is applicable in practice.…”

Section: Discussionmentioning

confidence: 99%

Differences of Enzymatic Activity During Composting and Vermicomposting of Sewage Sludge Mixed With Straw Pellets

2022

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The study aims were focused on profiling eight hydrolytic enzymes by fluorescence method using a multifunctional modular reader and studying the proportion of basic microorganism groups during composting and vermicomposting of sewage sludge mixed with straw pellets in several proportions (0, 25, 50, 75, and 100%). The greatest decrease in enzymatic activity occurred in the first half of composting and vermicomposting. After 4 months of these processes, the least enzymatic activity was observed in the sludge with 50% and also 25% straw addition, indicating that straw is an important means for the rapid production of mature compost from sewage sludge. Enzymatic activity was usually less in the presence of earthworms than in the control treatment because some processes took place in the digestive tract of the earthworm. For the same reason, we observed reduced enzyme activity during fresh feedstock vermicomposting than precomposted material. The final vermicompost from fresh feedstocks exhibited less microbial biomass, and few fungi and G− bacteria compared to precomposted feedstock. The enzymatic activity during composting and vermicomposting of sewage sludge and their mixtures stabilized at the following values: β-D-glucosidase—50 μmol MUFG/h/g dw, acid phosphatase—200 μmol MUFP/h/g dw, arylsulphatase—10 μmol MUFS/h/g dw, lipase—1,000 μmol MUFY/h/g dw, chitinase—50 μmol MUFN/h/g dw, cellobiohydrolase—20 μmol MUFC/h/g dw, alanine aminopeptidase—50 μmol AMCA/h/g dw, and leucine aminopeptidase—50 μmol AMCL/h/g dw. At these and lesser values, these final products can be considered mature and stable.

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“…Soil analysis. β-glucosidase activity in the root zone was determined following Hřebečková et al (2019) with several modifications. The lyophilised soil (0.2 ± 0.002 g) was mixed with 20 mL phosphate buffer (molar concentration 50 mmol/L, pH 7.0).…”

Section: Design Of the Experiments And Plant Materialmentioning

confidence: 99%

Change in β-glucosidase activity in root zone of ferns under toxic elements soil contamination

Novák¹,

Zemanová²,

Pavlı́k³

et al. 2023

PLANT SOIL ENVIRON

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Soil contamination with toxic elements affects soil microbiology and causes changes in the interactions between plants and microorganisms. It also significantly affects soil characteristics, plant growth, vegetation type, and agricultural land production (Wahsha et al. 2017, Zeng et al. 2019, Aponte et al. 2020a, Majumder et al. 2022. The accumulation of toxic elements by plants results in entry into the food chain, which then becomes hazardous to human health (Pande et al. 2022).Soil enzymes produced extracellularly by microorganisms are key participants in soil nutrient cycles and functional sustainability. They are sensitive to changes in the soil environment, such as contamination by toxic elements (Wahsha et al. 2017, Nkongolo and Narendrula-Kotha 2020, Cui et al. 2021, Majumder et al. 2022. Due to these characteristics, they can be used as biological indicators for evaluating soil health (Aponte et al. 2020b, Skowrońska et al. 2020.The mineralisation of carbon (C) in the soil is crucial for soil quality, as it increases soil fertility. However, this is influenced by plant root exudation, microbial activities, and soil pH (Yu et al. 2021). The zone of plant roots also influences the activity and

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Changes of enzymatic activity during a large-scale vermicomposting process with continuous feeding

Cited by 34 publications

References 31 publications

Problems associated with vermicomposting of dog excrement in practice using Eisenia andrei

Problems associated with vermicomposting of dog excrement in practice using Eisenia andrei

Differences of Enzymatic Activity During Composting and Vermicomposting of Sewage Sludge Mixed With Straw Pellets

Change in β-glucosidase activity in root zone of ferns under toxic elements soil contamination

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